Research Article

Gametocidal chromosomes enhancing chromosome aberration in common wheat induced by 5-azacytidine

Published: July 08, 2013
Genet. Mol. Res. 12 (3) : 2227-2233 DOI: 10.4238/2013.July.8.4

Abstract

The gametocidal (Gc) chromosome from Aegilops spp induces chromosome mutation, which is introduced into common wheat as a tool of chromosome manipulation for genetic improve­ment. The Gc chromosome functions similar to a restriction-modifi­cation system in bacteria, in which DNA methylation is an important regulator. We treated root tips of wheat carrying Gc chromosomes with the hypomethylation agent 5-azacytidine; chromosome break­age and micronuclei were observed in these root tips. The frequency of aberrations differed in wheat containing different Gc chromo­somes, suggesting different functions inducing chromosome break­age. Gc chromosome 3C caused the greatest degree of chromosome aberration, while Gc chromosome 3CSAT and 2C caused only slight chromosome aberration. Gc chromosome 3C induced different de­grees of chromosome aberration in wheat varieties Triticum aestivum var. Chinese Spring and Norin 26, demonstrating an inhibition func­tion in common wheat.

The gametocidal (Gc) chromosome from Aegilops spp induces chromosome mutation, which is introduced into common wheat as a tool of chromosome manipulation for genetic improve­ment. The Gc chromosome functions similar to a restriction-modifi­cation system in bacteria, in which DNA methylation is an important regulator. We treated root tips of wheat carrying Gc chromosomes with the hypomethylation agent 5-azacytidine; chromosome break­age and micronuclei were observed in these root tips. The frequency of aberrations differed in wheat containing different Gc chromo­somes, suggesting different functions inducing chromosome break­age. Gc chromosome 3C caused the greatest degree of chromosome aberration, while Gc chromosome 3CSAT and 2C caused only slight chromosome aberration. Gc chromosome 3C induced different de­grees of chromosome aberration in wheat varieties Triticum aestivum var. Chinese Spring and Norin 26, demonstrating an inhibition func­tion in common wheat.