Research Article

Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

Published: August 12, 2013
Genet. Mol. Res. 12 (3) : 2913-2922 DOI: https://doi.org/10.4238/2013.August.12.7
Cite this Article:
(2013). Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21. Genet. Mol. Res. 12(3): gmr2491. https://doi.org/10.4238/2013.August.12.7
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Abstract

An efficient Agrobacterium tumefaciens-mediated ge­netic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hy­gromycin B resistance expression vector, pCAMBIA1304’AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and opti­mized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.

An efficient Agrobacterium tumefaciens-mediated ge­netic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hy­gromycin B resistance expression vector, pCAMBIA1304’AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and opti­mized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.