Research Article

CYP1A2 polymorphism in Chinese patients with acute liver injury induced by Polygonum multiflorum

Published: July 25, 2014
Genet. Mol. Res. 13 (3) : 5637-5643 DOI: https://doi.org/10.4238/2014.July.25.19
Cite this Article:
(2014). CYP1A2 polymorphism in Chinese patients with acute liver injury induced by Polygonum multiflorum. Genet. Mol. Res. 13(3): gmr4170. https://doi.org/10.4238/2014.July.25.19
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Abstract

The objective of this study was to evaluate the genotype and allelic frequencies of CYP1A2 in Chinese patients with acute liver injury induced by Polygonum multiflorum. We examined the clinical mechanism of acute liver injury induced by P. multiflorum. According to the diagnostic criteria for drug-induced liver injury (DILI), 43 cases of P. multiflorum-induced liver injury admitted to the First Affiliated Hospital, Zhejiang University were identified between January 2008 and December 2012. An additional 43 control subjects were also chosen. Several alleles, including *1C, *1F, *2, *7, *9, and *11 of CYP1A2 were amplified from genomic DNA and sequenced. We used the chi-square test to determine whether CYP1A2 allele polymorphisms are associated with acute liver injury induced by P. multiflorum. The frequency of the CYP1A2 *1C allele was 46.5% in P. multiflorum-induced DILI patients, which was significantly different from the frequency of 27.9% observed in healthy subjects. The frequency of the CYP1A2*1F allele was 63.9% in P. multiflorum-induced DILI patients, compared to 57.0% in healthy controls; the difference was not significant. The allelic frequencies of CYP1A2*2, CYP1A2*7, CYP1A2*9, and CYP1A2*11 were too low to be detected. The frequency of the CYP1A2*1C mutation in Chinese patients with P. multiflorum-induced acute liver injury differed from that in healthy Chinese people, indicating that CYP1A2*1C is probably related to metabolism of P. multiflorum, which is followed by acute liver injury.

The objective of this study was to evaluate the genotype and allelic frequencies of CYP1A2 in Chinese patients with acute liver injury induced by Polygonum multiflorum. We examined the clinical mechanism of acute liver injury induced by P. multiflorum. According to the diagnostic criteria for drug-induced liver injury (DILI), 43 cases of P. multiflorum-induced liver injury admitted to the First Affiliated Hospital, Zhejiang University were identified between January 2008 and December 2012. An additional 43 control subjects were also chosen. Several alleles, including *1C, *1F, *2, *7, *9, and *11 of CYP1A2 were amplified from genomic DNA and sequenced. We used the chi-square test to determine whether CYP1A2 allele polymorphisms are associated with acute liver injury induced by P. multiflorum. The frequency of the CYP1A2 *1C allele was 46.5% in P. multiflorum-induced DILI patients, which was significantly different from the frequency of 27.9% observed in healthy subjects. The frequency of the CYP1A2*1F allele was 63.9% in P. multiflorum-induced DILI patients, compared to 57.0% in healthy controls; the difference was not significant. The allelic frequencies of CYP1A2*2, CYP1A2*7, CYP1A2*9, and CYP1A2*11 were too low to be detected. The frequency of the CYP1A2*1C mutation in Chinese patients with P. multiflorum-induced acute liver injury differed from that in healthy Chinese people, indicating that CYP1A2*1C is probably related to metabolism of P. multiflorum, which is followed by acute liver injury.

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