Research Article

In vitro differentiation of sperm from male germline stem cell

Published: April 10, 2015
Genet. Mol. Res. 14 (2) : 2964-2969 DOI: https://doi.org/10.4238/2015.April.10.5
Cite this Article:
(2015). In vitro differentiation of sperm from male germline stem cell. Genet. Mol. Res. 14(2): gmr4848. https://doi.org/10.4238/2015.April.10.5
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Abstract

In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observed.

In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observed.