Research Article

Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide

Published: April 13, 2015
Genet. Mol. Res. 14 (2) : 3330-3337 DOI: https://doi.org/10.4238/2015.April.13.12
Cite this Article:
L. Lu, S.M. Li, L. Zhang, X.Q. Liu, D.Y. Li, X.L. Zhao, Y.P. Liu (2015). Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide. Genet. Mol. Res. 14(2): 3330-3337. https://doi.org/10.4238/2015.April.13.12
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Abstract

The objective of this study was to evaluate the effect of vitamin D3 (VD3) on the regulation of chicken intestinal β-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At 4, 24, and 48 h post-injection, intestines were collected and RNA was isolated to measure the chicken β-defensin genes with putative vitamin D responsive elements using quantitative polymerase chain reaction. Expressions of all 7 chicken β-defensin genes was detectable in the intestines. Significant increases in GAL-6, -7 and -9 were found following LPS injection treatment at 4, 24, and 48 h post-injection, respectively, whereas VD3 injection did not affect the expression of any investigated genes under normal conditions. However, the expression of GAL-4, -5, -6, and -10 were synergistically upregulated by VD3 in combination with LPS. These results suggest that VD3 enhances the immune immunity during LPS challenge by inducing the expression of chicken β-defensin genes when birds are exposed to immune stressors.

The objective of this study was to evaluate the effect of vitamin D3 (VD3) on the regulation of chicken intestinal β-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At 4, 24, and 48 h post-injection, intestines were collected and RNA was isolated to measure the chicken β-defensin genes with putative vitamin D responsive elements using quantitative polymerase chain reaction. Expressions of all 7 chicken β-defensin genes was detectable in the intestines. Significant increases in GAL-6, -7 and -9 were found following LPS injection treatment at 4, 24, and 48 h post-injection, respectively, whereas VD3 injection did not affect the expression of any investigated genes under normal conditions. However, the expression of GAL-4, -5, -6, and -10 were synergistically upregulated by VD3 in combination with LPS. These results suggest that VD3 enhances the immune immunity during LPS challenge by inducing the expression of chicken β-defensin genes when birds are exposed to immune stressors.