Research Article

Effects of sex and age on chicken TBC1D1 gene mRNA expression

Published: July 13, 2015
Genet. Mol. Res. 14 (3) : 7704-7714 DOI: 10.4238/2015.July.13.16

Abstract

The objective of this study was to investigate the effects of sex and slaughter age of chickens on fatty acid composition and TBC1D1 gene expression in 4 different tissues: breast muscle, thigh muscle, abdominal fat, and subcutaneous fat. Sixty Erlang mountainous chickens (hybrid SD02 x SD03) were raised under the same conditions and slaughtered at 8, 10, and 13 weeks of age. The results showed that the sex of the animal significantly affected the content of arachidic acid (C20:0), sinapic (C22:1), linoleic (C18:2n-6), eicosapentaenoic (C20:5n-3), and docosahexaenoic acids (C22:6n-3), whereas other fatty acid contents were not affected. Age had a significant effect on most monounsaturated fatty acids, except for octadecenoic acid (C18:1). TBC1D1 mRNA was abundant in all tissues at all 3 ages of slaughter. Cocks exhibited higher TBC1D1 mRNA levels than hens in the thigh muscle and abdominal fat at 10 and 13 weeks, respectively.

The objective of this study was to investigate the effects of sex and slaughter age of chickens on fatty acid composition and TBC1D1 gene expression in 4 different tissues: breast muscle, thigh muscle, abdominal fat, and subcutaneous fat. Sixty Erlang mountainous chickens (hybrid SD02 x SD03) were raised under the same conditions and slaughtered at 8, 10, and 13 weeks of age. The results showed that the sex of the animal significantly affected the content of arachidic acid (C20:0), sinapic (C22:1), linoleic (C18:2n-6), eicosapentaenoic (C20:5n-3), and docosahexaenoic acids (C22:6n-3), whereas other fatty acid contents were not affected. Age had a significant effect on most monounsaturated fatty acids, except for octadecenoic acid (C18:1). TBC1D1 mRNA was abundant in all tissues at all 3 ages of slaughter. Cocks exhibited higher TBC1D1 mRNA levels than hens in the thigh muscle and abdominal fat at 10 and 13 weeks, respectively.