Research Article

Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing

Published: April 04, 2016
Genet. Mol. Res. 15(2): gmr7886 DOI: https://doi.org/10.4238/gmr.15027886
Cite this Article:
(2016). Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing. Genet. Mol. Res. 15(2): gmr7886. https://doi.org/10.4238/gmr.15027886
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Abstract

To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.751, respectively. These markers should be useful tools for genetic variation and conservation studies of B. schreberi.

To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.751, respectively. These markers should be useful tools for genetic variation and conservation studies of B. schreberi.