Research Article

CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study

Published: April 26, 2016
Genet. Mol. Res. 15(2): gmr6298 DOI: https://doi.org/10.4238/gmr.15026298
Cite this Article:
Y.L. Song, L. Wang, J.C. Ren, Z.H. Xu, Y.L. Song, L. Wang, J.C. Ren, Z.H. Xu, Y.L. Song, L. Wang, J.C. Ren, Z.H. Xu (2016). CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study. Genet. Mol. Res. 15(2): gmr6298. https://doi.org/10.4238/gmr.15026298
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Abstract

To date, no study has investigated the association between CYP1A2-163C/A polymorphism and bladder cancer risk in a Chinese population. Here, we extracted genomic DNA from peripheral white blood cells, and differentiated CYP1A2 alleles by polymerase chain reaction-based restriction fragment length polymorphism methods. Differences in genotype frequencies between the cases and controls were evaluated using a chi-square test. The odds ratio (OR) and its 95% confidence interval (CI) were calculated using an unconditional logistic regression model. This revealed that the -163A allele was present at a significantly increased frequency in bladder cancer patients compared to healthy controls (44.10 vs 22.25%, P < 0.001). The prevalence of CC genotype, CA genotype, and AA genotype was 34.91, 41.98, and 23.11% in bladder cancer patients, and 64.00, 27.50, and 8.5% in the controls, respectively. Therefore, significant differences in the frequencies of -163 genotypes were found between bladder cancer patients and controls (P < 0.001). We found that the AA genotype was significantly associated with increased bladder cancer risk (OR = 3.72; 95%CI = 1.55-7.16; P = 0.02), and the -163A carriers were at increased risk of bladder cancer in a multivariate COX regression model (OR = 4.89, 95%CI = 2.78-10.87, P = 0.01). We conclude that the CYP1A2-163C/A polymorphism is associated with increased susceptibility to bladder cancer in the Chinese population.

To date, no study has investigated the association between CYP1A2-163C/A polymorphism and bladder cancer risk in a Chinese population. Here, we extracted genomic DNA from peripheral white blood cells, and differentiated CYP1A2 alleles by polymerase chain reaction-based restriction fragment length polymorphism methods. Differences in genotype frequencies between the cases and controls were evaluated using a chi-square test. The odds ratio (OR) and its 95% confidence interval (CI) were calculated using an unconditional logistic regression model. This revealed that the -163A allele was present at a significantly increased frequency in bladder cancer patients compared to healthy controls (44.10 vs 22.25%, P CYP1A2-163C/A polymorphism is associated with increased susceptibility to bladder cancer in the Chinese population.

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