Research Article

Characterization of novel polymorphic genomic microsatellite markers of Boehmeria tricuspis (Hance) Makino

Published: April 27, 2016
Genet. Mol. Res. 15(2): gmr7882 DOI: https://doi.org/10.4238/gmr.15027882
Cite this Article:
(2016). Characterization of novel polymorphic genomic microsatellite markers of Boehmeria tricuspis (Hance) Makino. Genet. Mol. Res. 15(2): gmr7882. https://doi.org/10.4238/gmr.15027882
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Abstract

In the present study, 59 polymorphic microsatellite loci of Boehmeria tricuspis (Hance) Makino were developed from the specific length amplified fragment sequencing data library of genome. The number of alleles per locus ranged from two to five, and the observed and expected heterozygosities ranged from 0.0000 to 1.0000 and from 0.0769 to 0.6751, respectively. Among the 59 loci, 25 displayed significant deviations from Hardy-Weinberg expectations (P B. tricuspis (Hance) Makino, for providing further knowledge on its population differentiation, breeding system, and dispersal ability, as well as quantitative trait locus mapping. These markers could also be valuable genetic resources for closely related species.

In the present study, 59 polymorphic microsatellite loci of Boehmeria tricuspis (Hance) Makino were developed from the specific length amplified fragment sequencing data library of genome. The number of alleles per locus ranged from two to five, and the observed and expected heterozygosities ranged from 0.0000 to 1.0000 and from 0.0769 to 0.6751, respectively. Among the 59 loci, 25 displayed significant deviations from Hardy-Weinberg expectations (P B. tricuspis (Hance) Makino, for providing further knowledge on its population differentiation, breeding system, and dispersal ability, as well as quantitative trait locus mapping. These markers could also be valuable genetic resources for closely related species.

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