Research Article

Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds

Published: June 20, 2016
Genet. Mol. Res. 15(2): gmr7726 DOI: https://doi.org/10.4238/gmr.15027726
Cite this Article:
N. Yao, L. Ai, Y.Y. Dong, X.M. Liu, D.Z. Wang, N. Wang, X.W. Li, F.W. Wang, X.K. Li, H.Y. Li, C. Jiang, N. Yao, L. Ai, Y.Y. Dong, X.M. Liu, D.Z. Wang, N. Wang, X.W. Li, F.W. Wang, X.K. Li, H.Y. Li, C. Jiang (2016). Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds. Genet. Mol. Res. 15(2): gmr7726. https://doi.org/10.4238/gmr.15027726
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Abstract

Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest significantly affected the protein yield and quality. Our results indicate that the Phaseolus vulgaris β-phaseolin promoter directed anti-TNF-α scFv-Fc expression in A. thaliana seeds, with a maximum yield obtained at 20-days of development. Although the yield of anti-TNF-α scFv-Fc protein was not very high, accumulation of recombinant proteins in seeds is an attractive and simple method that can be used to purify biologically active anti-TNF-α scFv-Fc.

Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest significantly affected the protein yield and quality. Our results indicate that the Phaseolus vulgaris β-phaseolin promoter directed anti-TNF-α scFv-Fc expression in A. thaliana seeds, with a maximum yield obtained at 20-days of development. Although the yield of anti-TNF-α scFv-Fc protein was not very high, accumulation of recombinant proteins in seeds is an attractive and simple method that can be used to purify biologically active anti-TNF-α scFv-Fc.