Research Article

Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

Published: August 12, 2016
Genet. Mol. Res. 15(3): gmr7993 DOI: 10.4238/gmr.15037993

Abstract

The Chinese fire-bellied newt, Cynops orientalis, belonging to Amphibia, Caudata, Salamandridae is a species endemic to China. The liver, which is an important digestive gland and the largest amphibian organ, has various functions, including detoxification, glycogen storage, protein synthesis, and hormone production. However, the newt liver has rarely been studied at the molecular level. We performed histomorphology and high-throughput proteomic analysis of the Chinese fire-bellied newt liver, using hematoxylin and eosin (H&E) staining and two-dimensional electrophoresis coupled with mass spectrometry. The H&E staining showed that the newt liver nuclei are large and round, are located in the lateral cytoplasm, and contain a large quantity of lipid droplets. Melanins were abundantly present throughout the hepatic parenchyma. The proteome analysis showed a total of 545 proteins detected in the newt liver. Furthermore, a gene ontology analysis suggested that these proteins were associated with metabolism, immune response, cellular homeostasis, etc. Among these, proteins with metabolic functions were found to be the most abundant and highly expressed. This supports the role of the liver as the metabolic center. The proteomic results provide new insights into the aspects of the liver proteomes of the Chinese fire-bellied newt. The identification of a more global liver proteome in the newt may provide a basis for characterizing and comparing the liver proteomes from other amphibian species.

The Chinese fire-bellied newt, Cynops orientalis, belonging to Amphibia, Caudata, Salamandridae is a species endemic to China. The liver, which is an important digestive gland and the largest amphibian organ, has various functions, including detoxification, glycogen storage, protein synthesis, and hormone production. However, the newt liver has rarely been studied at the molecular level. We performed histomorphology and high-throughput proteomic analysis of the Chinese fire-bellied newt liver, using hematoxylin and eosin (H&E) staining and two-dimensional electrophoresis coupled with mass spectrometry. The H&E staining showed that the newt liver nuclei are large and round, are located in the lateral cytoplasm, and contain a large quantity of lipid droplets. Melanins were abundantly present throughout the hepatic parenchyma. The proteome analysis showed a total of 545 proteins detected in the newt liver. Furthermore, a gene ontology analysis suggested that these proteins were associated with metabolism, immune response, cellular homeostasis, etc. Among these, proteins with metabolic functions were found to be the most abundant and highly expressed. This supports the role of the liver as the metabolic center. The proteomic results provide new insights into the aspects of the liver proteomes of the Chinese fire-bellied newt. The identification of a more global liver proteome in the newt may provide a basis for characterizing and comparing the liver proteomes from other amphibian species.