Research Article

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Published: October 13, 2009
Genet. Mol. Res. 8 (4) : 1238-1244 DOI: https://doi.org/10.4238/vol8-4gmr641
Cite this Article:
Y. Erturk, S. Ercisli, D. Maghradze, E. Orhan, G. Agar (2009). An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers. Genet. Mol. Res. 8(4): 1238-1244. https://doi.org/10.4238/vol8-4gmr641
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Abstract

Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.

Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.