Research Article

Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny

Published: November 03, 2009
Genet. Mol. Res. 8 (4) : 1323-1330 DOI: https://doi.org/10.4238/vol8-4gmr674
Cite this Article:
N.M.A. Nassar, P.T.C. Gomes, A.M. Chaib, N.N. Bomfim, R.C.D. Batista, R.G. Collevatti (2009). Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny. Genet. Mol. Res. 8(4): 1323-1330. https://doi.org/10.4238/vol8-4gmr674
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Abstract

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical with each other and with their maternal progenitor. Sibs of selfed M. glaziovii proved to be identical when examined with these microsatellite loci. The chromosome complement of the apomictic clone was 2n = 38. We observed multi-embryonic aposporic embryo sacs.

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical with each other and with their maternal progenitor. Sibs of selfed M. glaziovii proved to be identical when examined with these microsatellite loci. The chromosome complement of the apomictic clone was 2n = 38. We observed multi-embryonic aposporic embryo sacs.