Research Article

Transfer of microsatellite markers from other Arecaceae species to Syagrus romanzoffiana (Arecaceae)

Published: July 18, 2019
Genet. Mol. Res. 18(3): GMR18183 DOI: 10.4238/gmr18183

Abstract

The species Syagrus romanzoffiana, a native palm tree of South America, is widely distributed and well adapted to southern Brazil. It is an important economic, cultural, and ecological resource, being the preferred food of diverse animal species, involving complex ecological relationships. However, despite numerous molecular studies on native plants, specific molecular markers, such as expressed sequence tag-simple sequence repeats (EST-SSRs), are lacking, and there are few SSR markers for this species. Molecular data about individuals and their populations offers new perspectives for management, conservation, and economic exploitation. Here we tested 21 microsatellite loci known from three species of the family Arecaceae in three native populations of S. romanzoffiana in the state of Rio Grande do Sul, in southern Brazil. Among the 21 primer pairs tested, 12 produced an amplification product. Five loci were chosen and subjected to diversity tests. The populations presented between three and nine alleles per locus, and acceptable genetic diversity values; the expected heterozygosity ranged from 0.460 to 0.864, while the observed heterozygosity ranged from 0.100 to 1.000. We conclude that various microsatellite markers developed for other species of the family Arecaceae are suitable for molecular analysis of S. romanzoffiana populations.

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