Viability of simultaneous genotypic screening and germination of individual seeds in rice, beans and maize
DNA-assisted selection can be applied to vegetal species in the seed stage; however, little is known about the effect of seed fractionation on the physiological quality and viability of the seedlings or the effectiveness of DNA extraction from seed pieces. We evaluated the efficiency of pre-germinative genotypic screening by DNA markers from manually cut partial seeds of rice, beans and maize. Tests to evaluate PCR amplification and physiological quality were performed. We observed that the Sarkosyl method was efficient to extract DNA from a ½ fraction of the rice seeds and ¼ of the bean and maize seeds, generating good quality SSR-PCR products. The physiological quality of the rice seeds cut in half and the bean and maize seeds remaining fraction of ¾ of the original seed provided a high germination percentage. The method is effective for simultaneously genotyping and germinating plants from a single seed, since DNA extracted from these fractions of seeds can be used for studies with DNA markers, while the remaining portions can be used for seedling production.