Publications of : N.M.A. Nassar
Plant Genetics   Research Article

Periclinal chimera technique: new plant breeding approach

Plant interspecific periclinal chimeras are a mosaic formed by tissues from two species. They are manipulated here as an efficient plant breeding tool for cassava root yields. In this study, plants synthesized from two chimeras, designated as chimera 2 and chimera 4, were characterized morphologically and cytologically to unravel the origin of their tissue layers (L.. Read More»

Genet. Mol. Res. 16(3): gmr16039790

DOI: 10.4238/gmr16039790

Plant Genetics   Research Article

Cytogenetic and anatomic behavior of cytochimeras and total polyploids in cassava

    D.Y. Hashimoto-Freitas1 and N.M.A. Nassar2

Cassava periclinal cytochimeras, cultivars, and interspecific hybrid and polyploid types were studied in relation to embryonic, cytogenetic, and anatomical behavior. Their apical shoots, pollen grains, male and female buds, roots, stomata, and flowering period were analyzed. Chimeras exhibited increased size of L1 and L2 cells. Polyploidy led to enlargement of stomata in chimeras whereas L2 gav.. Read More»

Genet. Mol. Res. 12(4): 2013.October.22.7

DOI: 10.4238/2013.October.22.7

Plant Genetics   Research Article

Synthesis of periclinal chimera in cassava

    N.M.A. Nassar N. Bomfim

We provide the first report on the synthesis of a very productive interspecific periclinal chimera of cassava, with large and edible roots. The epidermal tissue of the chimera was formed by the cultivated species Manihot esculenta (E), and the subepidermis and internal tissue were formed by the wild species, Manihot fortalezensis (F). We used cytogenetics and morphological analyses to determine th.. Read More»

Genet. Mol. Res. 12(1): 2013.February.27.10

DOI: 10.4238/2013.February.27.10

Plant Genetics   Research Article

Genetic, embryonic and anatomical study of an interspecific cassava hybrid

    N.M.A. Nassar, D.Y. Hashimoto and D.G. Ribeiro

A molecular, anatomical and cytogenetic study of an interspecific hybrid between Manihot esculenta (cassava) and the wild species M. oligantha was carried out. Cytogenetics revealed relatively complete chromosome pairing and high viability of the pollen grains. Ovule structure examined by the clearing method showed polyembryony in 2.7% of the ovules. Doubling of the.. Read More»

Genet. Mol. Res. 9(1): vol9-1gmr746

DOI: 10.4238/vol9-1gmr746

Plant Genetics   Research Article

Apomixis and cassava

    N.M.A. Nassar

Apomixis means seed formation without fertilization. In cassava (Manihot esculenta) it is an alternative to reproduction by cuttings, which normally transmits pathogens and leads to an accumulation of viral and bacterial diseases. Apomixis also assures preservation of heterosis and avoids genetic segregation. It occurs in wild relatives of cassava and has been trans.. Read More»

Genet. Mol. Res. 1(2):

Plant Genetics   Research Article

Amarelinha do Amapá: a carotenoid-rich cassava cultivar

    N.M.A. Nassar, P.C. Fernandes, R.D. Melani and O.R. Pires Júnior

Eight cassava (Manihot esculenta Crantz) cultivars, of which four are indigenous to the Amazon region, along with an interspecific hybrid, were assessed for their carotenoid content. They were propagated and are maintained in the living Manihot col­lection at the University of Brasília. The cultivar “Amarelinha do Amapá”, which was collected from the State of Amap&.. Read More»

Genet. Mol. Res. 8(3): vol8-3gmr625

DOI: 10.4238/vol8-3gmr625

Cytogenomics   Research Article

Cassava interspecific hybrids with increased protein content and improved amino acid profiles

    P.T.C. Gomes and N.M.A. Nassar

Cassava (Manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. Its edible roots are poor in protein and lack several essential amino acids. Interspecific hybrids may acquire high protein characteristics from wild species. We analyzed 19 hybrids of M. esculenta with its wild relative, M. oligantha, for crude protein, amino acid profile, and to.. Read More»

Genet. Mol. Res. 12(2): 2013.April.12.8

DOI: 10.4238/2013.April.12.8

Plant Genetics   Research Article

Cassava, Manihot esculenta Crantz, genetic resources: origin of the crop, its evolution and relationships with wild relatives

    N.M.A. Nassar

About 98 species of Manihot are known. All of them are native to the New World and are concentrated in four regions in Brazil and Central America. All the Manihot species so far examined have 2n = 36 chromosomes. Interspecific hybrids between cassava and its wild relatives show relatively normal meiosis, and further generations can be obtained. Electrophoresis shows.. Read More»

Genet. Mol. Res. 1(4):

Human Genetics   Research Article

Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny

    N.M.A. Nassar, P.T.C. Gomes, A.M. Chaib1, N.N. Bomfim,R.C.D. Batista and R.G. Collevatti

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical wi.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr674

DOI: 10.4238/vol8-4gmr674

Microbial Genetics   Research Article

Cassava, Manihot esculenta Crantz genetic resources: VI. Anatomy of a diversity center

    N.M.A. Nassar

Central Brasil is one of the four centers of diversity of Manihot species. Of 26 wild Manihot species reported to occur in this region, 20 were collected from two limited areas, each less than 100 km in diameter. These two areas are Goiás Velho and Corumbá de Goiás. Considering the Harlan concept of geographic patterns of variation of cultivated.. Read More»

Genet. Mol. Res. 2(2):

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