Table of contents: 2017 ( Volume 16, Issue 4 )
Animal Genetics   Research Article

Comparison of three methods for diagnosis of Trypanosoma (Duttonella) vivax in cattle

Detection of Trypanosoma vivax in cattle is based on parasitological, serological and molecular methods. The aim of this study was to compare three different methods for detecting T. vivax during an outbreak of trypanosomiasis in southeast Brazil. Blood samples were collected from seventy-two animals to perform micro-hematocrit centrifugation technique (MHCT), indir.. Read More»

Genet. Mol. Res. 16(4): gmr16039811

DOI: 10.4238/gmr16039811

Microbial Genetics   Research Article

Development of 21 novel polynucleotide microsatellite markers in a Clithon retropictum (Gastropoda: Neritidae), using next-generation sequencing

The neritid species Clithon retropictum (Gastropoda), an oviparous snail, is distributed in freshwater and brackish water in southern Korea, south-east Japan, southern China and Taiwan. This species is listed as a class II endangered species in Korea because of its decreasing population size. It has led to an increase in the need for genetic information about this s.. Read More»

Genet. Mol. Res. 16(4): gmr16039806

DOI: 10.4238/gmr16039806

Human Genetics   Research Article

IGF2BP2 gene polymorphism in the pathogenesis of vitiligo

Vitiligo is a disorder of pigmentation presenting with loss of melanocytes in the epidermis and hair follicles. The gene for insulin-like growth factor binding protein 2 (IGF2BP2) is located on the third chromosome and it plays role in growth, development, cellular differentiation, and metabolism. In the present study, we aimed to investigate the rs1470579 and rs440.. Read More»

Genet. Mol. Res. 16(4): gmr16039808

DOI: 10.4238/gmr16039808

Human Genetics   Research Article

AluYb8 insertion in the WNK1 gene is not associated with hypertension in a Russian Caucasian population

WNK1 (With No-lysine Kinase 1), a serine-threonine kinase, regulates blood pressure by acting on various sodium transport-related ion channels. Several studies report a link between common variants of the WNK1 gene and hypertension. No data exists on Russian populations. Our aim was to evaluate the association between the WNK1 AluYb8 polymorphism and hypertension su.. Read More»

Genet. Mol. Res. 16(4): gmr16039809

DOI: 10.4238/gmr16039809

Human Genetics   Research Article

Factor V Leiden G1691A and Prothrombin G20210A mutations are associated with repeated spontaneous miscarriage in Northern area of Saudi Arabia

Maternally inherited thrombophilia could be one of the causes of recurrent spontaneous miscarriage (RSM). We aimed to investigate the frequency of three thrombotic gene variants; factor V Leiden (FVL; G1691A), prothrombin (PTH; G20210A), and methylenetetrahydrofolate reductase (MTHFR; C677T) in Saudi patients diagnosed with RSM. A case control study was conducted on.. Read More»

Genet. Mol. Res. 16(4): gmr16039810

DOI: 10.4238/gmr16039810

Plant Genetics   Research Article

Genome-Wide Analysis of a Radiation-Induced Lesion Mimic Mutant in Rice

Lesion mimic mutations (LMMs) are very useful genetic tools in plants, however, it is difficult to uncover genome-wide mutations using traditional technology, the development of whole genome sequencing (WGS) provides a means by which to identify genome-wide LMMs. In this study, WGS was performed to reveal the molecular mechanisms behind LMMs in rice. An LMM JA001 wa.. Read More»

Genet. Mol. Res. 16(4): gmr16039812

DOI: 10.4238/gmr16039812

Plant Genetics   Research Article

Genetic Variability for Sprout Growth among Genotypes of Coffea canephora Led by Bending of Orthotropic Stems

The multi-stem aspect of crop systems using Coffea canephora makes it necessary to correctly establish the number of orthotropic stems per plant during the crop formation. The present study was developed to study the variability of responses among improved genotypes of C. canephora to the technique of bending orthotropic stems as a mean of promoting the sprout growt.. Read More»

Genet. Mol. Res. 16(4): gmr16039813

DOI: 10.4238/gmr16039813

Plant Genetics   Research Article

Physiological Traits and Their Relationships in Black Oat Populations

The objective of this work was to verify physiological quality of black oat populations, in order to know and point out the relationships among physiological characters for selection of progenies. The experiment was carried out at the Laboratory of Plant Breeding and Production, Federal University of Santa Maria, Campus of Frederico Westphalen - RS. Seed samples wer.. Read More»

Genet. Mol. Res. 16(4): gmr16039814

DOI: 10.4238/gmr16039814

Plant Genetics   Research Article

Characterization of the complete Chloroplast genome of Correa carmen, a valuable winter-flowering shrub

Correa carmen is considered important because of its considerable ornamental and economic value. The most striking characteristic of C. carmen flowers is their long winter-flowering period. In the present study, we generated the first complete C. carmen chloroplast genome sequence based on Illumina paired-end sequencing data. The entire chloroplast genome comprises .. Read More»

Genet. Mol. Res. 16(4): gmr16039815

DOI: 10.4238/gmr16039815

Human Genetics   Research Article

Comparative Study of Blood-Derived Scaffolds for the Culture of Human Adipose Derived Stem Cells (ASCS) and Dermal Fibroblasts

This study aimed to compare the performance of bloodderived scaffolds with a well-known and accepted scaffold, chitosan, in maintaining cell cultures of ASCs and fibroblasts for future wound healing applications. Cells were characterized, immunophenotyped and cultivated into the following scaffolds: 1) Chitosan (CH, control), 2) Platelet gel (PG), 3) Chitosan blende.. Read More»

Genet. Mol. Res. 16(4): gmr16039807

DOI: 10.4238/gmr16039807

Human Genetics   Research Article

DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer

Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissues from patients with colorectal cancer .. Read More»

Genet. Mol. Res. 16(4): gmr16039816

DOI: 10.4238/gmr16039816

Plant Genetics   Research Article

Genetics Analysis of the Biggest Cashew Tree in the World

This study performed a genetic study of several canopies of Anacardium occidentale L. specimen called "Cashew King" (Cajueiro da Praia-PI, Brazil) in order to assess whether all canopies belong to the same plant. Leaves were collected from  different distant points, and preserved for analysis of DNA. The analysis was made from automated sequencing by .. Read More»

Genet. Mol. Res. 16(4): gmr16039817

DOI: 10.4238/gmr16039817

Human Genetics   Research Article

Promotion of apoptosis in high glucose-activated hepatic stellate cells by GLP-1 receptor agonist and its potential mechanism

This study aimed to investigate the stimulatory effect of glucagon-like peptide 1 (GLP-1) receptor agonist (GLP-1RA) on the apoptosis of hepatic stellate cells (HSCs) activated by high glucose, and to explore the underlying molecular mechanism with a focus on the c-Jun N-terminal kinase (JNK) and extracellular signal-related kinase (ERK) signaling pathways. Human HS.. Read More»

Genet. Mol. Res. 16(4): gmr16039818

DOI: 10.4238/gmr16039818

Animal Genetics   Research Article

BmICE-2 interference vector construction and its silencing effect in the BmN-SWU1 cells

Investigate the function of BmICE-2 gene in silkworm cells by constructing a BmICE-2 interference vector and analysing its interference effect. Online RNAi design software was used to predict the interference target sequence of Bmice-2. An optimized sequence was selected and inserted into the backbone of interference microRNA. The backbone of interference vector, mi.. Read More»

Genet. Mol. Res. 16(4): gmr16039819

DOI: 10.4238/gmr16039819

Plant Genetics   Research Article

Differential expression of dormancy-Associated genes in fastigiata and hypogaea peanut

Seed dormancy is a temporary replacement state observed in some higher plants, which prevents germination under unfavorable conditions. In several species, dormancy process is triggered by hormonal factors, initiated by ABA and precursor enzymes. In this work, we investigated the relationship between dormancy and the expression of ARP, DMR1, and NCED genes in uprigh.. Read More»

Genet. Mol. Res. 16(4): gmr16039820

DOI: 10.4238/gmr16039820

Human Genetics   Research Article

Genotypes within the prolactin and growth hormone insulin-like growth factor-I pathways associated with milk production in heat stressed Holstein cattle: Genotypes and milk yield in heat stressed Holstein cows

The association of single nucleotide polymorphisms (SNPs) within genes of the prolactin (PRL), growth hormone (GH) and insulin-like growth factor-I (IGF1) pathways with 305-day milk yield (MY305) were evaluated in heat-stressed Holstein cows. Ambient temperature and relative humidity were used to calculate a temperature-humidity index (THI), which revealed that heat.. Read More»

Genet. Mol. Res. 16(4): gmr16039821

DOI: 10.4238/gmr16039821

Human Genetics   Research Article

Case report of X-linked hyper-IgM syndrome with acquired toxoplasmic encephalitis

The X-linked hyper-IgM (XHIGM; HIGM1) syndrome is an uncommon primary immunodeficiency disease caused by mutations in the gene for CD40 ligand and characterized by normal or elevated serum IgM, low or absent serum levels of IgG, IgA and IgE, and defective T-cell function. Here we reported a case of XHIGM with acquired toxoplasmic encephalitis. The patient experience.. Read More»

Genet. Mol. Res. 16(4): gmr16039822

DOI: 10.4238/gmr16039822

Human Genetics   Research Article

Clinical and molecular findings in an Albanian family with familial adenomatous polyposis

Purpose: Familial adenomatous polyposis is an inherited precancerous condition characterized by multiple colorectal polyps. This brief report describes three generations of a family with a history of colorectal cancer in which genetic testing was useful for detecting individuals at risk. Methods: A next generation sequencing custom gene panel comprising 11 genes ass.. Read More»

Genet. Mol. Res. 16(4): gmr16039823

DOI: 10.4238/gmr16039823

Animal Genetics   Research Article

Epidemiological and pathological studies on the helminthic parasites in native chickens of Tabriz city, Iran

A study of intestinal helminthic parasites of domestic chickens was carried out in Tabriz city, Iran during 2016. One hundred (100) gastrointestinal samples were examined in necropsied chickens microscopically using the lactophenol and carmine-acid staining technique. The result showed a high prevalence of intestinal parasitic infection. Out of 100 domestic chicken,.. Read More»

Genet. Mol. Res. 16(4): gmr16039824

DOI: 10.4238/gmr16039824

Animal Genetics   Research Article

Transferability of heterologous microsatellite loci between species of Euterpe genus

Euterpe precatoria, popularly known as “açaí-do-amazonas”, has agronomic, technological and economic potential and has been gaining prominence with the commercialization of its fruits. The production of this açaí-do-amazonas is based on extractivism and management practices have been recommended for sustainable collection. To .. Read More»

Genet. Mol. Res. 16(4): gmr16039825

DOI: 10.4238/gmr16039825

Human Genetics   Research Article

Mitochondrial diversity in the populations of Pediculus humanus capitis across eastern south of Tehran Province, Iran

Pediculus humanus capitis is a major obligate and district ecto-parasite of human which is distributed in different parts of the world and is under several research projects for population management and molecular analysis. In this study, one hundred and seventy head lice were collected from school girls at different high schools in five cities in south east of Tehr.. Read More»

Genet. Mol. Res. 16(4): gmr16039826

DOI: 10.4238/gmr16039826

Human Genetics   Research Article

Promotion of apoptosis in high glucose-activated hepatic stellate cells by GLP-1 receptor agonist and its potential mechanism.

This study aimed to investigate the stimulatory effect of glucagon-like peptide 1 (GLP-1) receptor agonist (GLP-1RA) on the apoptosis of hepatic stellate cells (HSCs) activated by high glucose, and to explore the underlying molecular mechanism with a focus on the c-Jun N-terminal kinase (JNK) and extracellular signal-related kinase (ERK) signaling pathways. Human HS.. Read More»

Genet. Mol. Res. 16(4): gmr16039818

DOI: 10.4238/gmr16039818

Stay informed on our latest news!