Table of contents: 2018
Animal Genetics   Research Article

Characterization of 33 microsatellite markers and development of multiplex PCR for yellow-throated marten (Martes flavigula)

Authors: Mu-Yeong Lee, Hea Chang Moon, Hey Sook Jeon, Eui-Geun Song, Donggul Woo, HeeBok Park, Tae-Young Choi, Kyung-Yeon Eo, Yongsun Hyun, Kangbok Lee, Young-Seok Park, Ju-Young Moon, Hyun-Jin Han, Junghwa An

The yellow-throated marten (Martes flavigula Boddaert 1785) is a medium-sized carnivore and a top predator in South Korea that is distributed throughout Western and Southeast Asia and Siberia in a wide range of habitats. In this study, we developed a panel of polymorphic microsatellite markers for M. flavigula by Illumina next-generation sequencing for investigation.. Read More»

Genet. Mol. Res. 17(1): gmr16039854

DOI: 10.4238/gmr16039854

Plant Genetics   Research Article

Cloning and expression analysis of pistillata-like gene in Sedirea japonica

Authors: Suhua Jiang, Suyan Niu, Xiuyun Yuan, Fang Liang, Shenping Xu, Mofei Wang, Bo Cui

Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea japonica (designated PI-like, GenBank No... Read More»

Genet. Mol. Res. 17(1): gmr16039855

DOI: 10.4238/gmr16039855

Plant Genetics   Research Article

Parapatric speciation found in Limnonectes (Dicroglossidae) species in Thailand

Authors: Pimwipa Teerawarunyoo, Chatmongkon Suwannapoom, Phuping Sucharitakul, Kittisak Buddhachat, Waranee Pradit, Korakot Nganvongpanit and Siriwadee Chomdej

Biodiversity stems from speciation, and species identification allows scientists to measure species biodiversity. However, some closely related species, particularly amphibians that choose to inhabit the same environment, are difficult to identify. Juvenile specimens of Limnonectes gyldenstolpei (Lg) and L. taylori (Lt) were used as models to study the speciation pr.. Read More»

Genet. Mol. Res. 17(1): gmr16039856

DOI: 10.4238/gmr16039856

Microbial Genetics   Research Article

LC-MS based identification of secondary metabolites from marine antagonistic endophytic bacteria

Authors: Fehmida Bibi, Muhammad Imran Naseer, Muhammad Yasir, Ahmed Abdullah Khalaf Al-Ghamdi, Esam Ibrahim Azhar

Halophytes occupy coastal and sub-coastal area of marine environment. They potential candidates for search of novel and new bacterial flora that have immense potential to yield novel therapeutic agents. Six different endophytic bacteria have been isolated from pneumatophores and roots of three halophytes (Salsola imbricata, Avicennia marina and Haplopeplis perfoliat.. Read More»

Genet. Mol. Res. 17(1): gmr16039857

DOI: 10.4238/gmr16039857

Human Genetics   Research Article

A novel homozygous stop gain mutation in SLC12A3 gene cause Gitelman syndrome in Saudi consanguineous family

Authors: Muhammad Imran Naseer, Osama Yousef Muthaffar , Mahmood Rasool, Angham Abdulrahman Abdulkareem, Mohammad Alam Jafri, Peter Natesan Pushparaj, Gauthaman Kalamegam, Adeel G. Chaudhary, Mohammad H. Al-Qahtani

Gitelman syndrome (GS) is a genetic disorder that affects kidney and causes an imbalance of charged atoms (ions) in the body, including ions of potassium, magnesium, and calcium. GS is characterized by hypokalemia and metabolic alkalosis. GS is a rare autosomal recessive renal tubulopathy disease caused by loss-of-function mutations in the SLC12A3 gene. Objective of.. Read More»

Genet. Mol. Res. 17(1): gmr16039858

DOI: 10.4238/gmr16039858

Animal Genetics   Research Article

Novel polymorphisms in bovine CD4 and LAG-3 genes associated with somatic cell counts of clinical mastitis cows

Authors: Tahir Usman, Yachun Wang, Minyan Song1, Xiao Wang, Yichun Dong, Chao Liu, Shuxiang Wang, Yi Zhang, Wei Xiao, Ying Yu

Clinical mastitis cows normally produce clotted milk, thus the much higher somatic cells in milk are unable to be counted by routine FOSS machine. The proteins coded by CD4 and LAG-3 genes can bind to MHC class II molecules and play important roles in in-flammatory diseases. The present study was designed to investigate the effects of single nucleotide polymorphisms.. Read More»

Genet. Mol. Res. 17(1): gmr16039859

DOI: 10.4238/gmr16039859

Human Genetics   Research Article

Biophysical and biochemical mechanisms disturbance of cellular genome leading to transmutation benign neoplasm into cancer

Authors: Ponizovskiy M.R.

It was considered processes transmutation of genome mechanism from the point of view of thermodynamics, biophysics, and biochemistry. Just it was explained mechanisms maintenance stability Internal Energy of Stationary State an able-bodied organism according first law of thermodynamics. Besides it was considered some negative influences on an organism of solar radia.. Read More»

Genet. Mol. Res. 17(1): gmr16039860

DOI: 10.4238/gmr16039860

Plant Genetics   Research Article

Large-scale development, characterization, and cross-amplification of EST–SSR markers in Chinese chive

Authors: Qingming Tang, Langbo Yi, Xianglin Yuan, Fu Li

Chinese chive (Allium tuberosum Rottler ex Spr.) is an important vegetable crop. However, genetic and breeding studies of the species have been restricted by the lack of simple sequence repeat markers (SSRs). In the present study, a total of 2553 Chinese chive SSRs were developed from the species’ transcriptome, with 626, 643, and 536 of the makers located in .. Read More»

Genet. Mol. Res. 17(1): gmr16039861

DOI: 10.4238/gmr16039861

Plant Genetics   Research Article

Genetic diversity as tool to identify standard leaf nutrients in cassava genotypes

Authors: JAD Giles, WP Rodrigues, G Oliosi, FL Partelli, HD Vieira and Vanderlei da Silva Santos

Among the factors affecting cassava yield, the leaf nutrients content has received little attention since cassava genotypes may grow well under inappropriate nutrient supply, however showing inadequate nutritional quality of tuberous roots. Considering this aspect, the genetic diversity should be explored in order to determine standard leaf nutrients in cassava geno.. Read More»

Genet. Mol. Res. 17(1): gmr16039862

DOI: 10.4238/gmr16039862

Plant Genetics   Research Article

Isolation and cross-amplification of fifteen microsatellites for oil-rewarding Calceolaria species (Calceolariaceae)

Authors: M. Murúa, G. Peralta, L. Carrasco, and F. Pérez

Calceolaria is a diversified American genus whose species are mainly visited by oil-collecting bees, representing a highly specialized plant-pollinator system. However, although this is a unique plant system, different aspects of its ecology and evolution remain unexplored. In this study we characterized fifteen polymorphic microsatellites for four Andean Calceolari.. Read More»

Genet. Mol. Res. 17(1): gmr16039863

DOI: 10.4238/gmr16039863

Plant Genetics   Research Article

Ectopic Expression of AtCIPK23 Enhances Drought Tolerance via Accumulating Less H2O2 in Transgenic Tobacco Plants

Authors: Liming Lu, Linglong Yang, Ke Li, Yifei Lu, Liqin Li

Drought is a major threat to agricultural crop growth and productivity. However, the molecular mechanism behind such effects remains largely unknown. Plant CBL (Calcineurin B-Like)-interacting protein kinases (CIPKs) are believed to play an important role in plant drought tolerance and signalling transduction. Here, we report the influence of a member of the CIPK fa.. Read More»

Genet. Mol. Res. 17(1): gmr16039864

DOI: 10.4238/gmr16039864

Plant Genetics   Research Article

Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers

Authors: J.C. Costa, G.G.M. Fracetto, F.J.C. Fracetto, T.C. Souza, M.V.F. Santos and M.A. Lira Júnior

Genetic diversity is the basis for genetic improvement, providing genes for new cultivars. Stylosanthes scabra is a legume used in pastures, presenting economic importance in tropical and subtropical regions. Molecular markers are targeted in population studies, thus we evaluated the genetic diversity distribution of S. scabra in Brazilian semi-arid northeastern amo.. Read More»

Genet. Mol. Res. 17(1): gmr16039866

DOI: 10.4238/gmr16039866

Plant Genetics   Research Article

Characterization of aapP and nopP genes related to the biological nitrogen fixation efficiency with soybean in contrasting strains of Bradyrhizobium japonicum

Authors: J.G. Perin, M.B.D. Bertéli, J.S. do Valle, G.A. Linde, L.D. Paccola-Meirelles, N.B. Colauto and F.G. Barcellos

Soybean (Glycine max) is considered one of the most economically important agricultural crops in Brazil. Due to the high protein content of its grains, this crop demands a greater amount of nitrogen (N) for production. In general, for soybean, nitrogen fertilization costs through chemical fertilizers are considered high. However, the biological nitrogen fixation (BN.. Read More»

Genet. Mol. Res. 17(1): gmr16039867

DOI: 10.4238/gmr16039867

Animal Genetics   Research Article

Effect of genetic polymorphism of αS1- casein gene on qualitative and quantitative milk traits in native Bulgarian Rhodopean cattle breed

Authors: J Peter Hristov, Denitsa Teofanova, Ani Georgieva, Georgi Radoslavov

Milk protein genetic polymorphisms of the genus Bos provoke a significant scientific interest, mainly associated with their evolution, population structure, breeding and hybridization. The aim of present study is to investigate the influence of the genetic variants of αS1- casein gene with respect to milk production and quality of native for Bulgaria cattle br.. Read More»

Genet. Mol. Res. 17(1): gmr16039868

DOI: 10.4238/gmr16039868

Plant Genetics   Research Article

Determination of the number of harvests to select elite sugarcane genotypes in NE Brazil

Authors: J.A. Dutra Filho, T.C. Junior, D. E. Simoes Neto, L. S. Souto, Romulo Gil de Luna A.S. Souza

Sixty-eight sugarcane genotypes were evaluated to determine the number of harvests required to select new genotypes in the sugarcane belt in the state of Pernambuco, northeast Brazil. Using a random block design, the following parameters were evaluated: (i) polarizable sugars (POL) per hectare, in metric tons (TPH); (ii) culm productivity per hectare (TCH); (iii) fi.. Read More»

Genet. Mol. Res. 17(1): gmr16039869

DOI: 10.4238/gmr16039869

Plant Genetics   Research Article

Genetic diversity assessment of Allium cepa L. cultivars from Bosnia and Herzegovina using SSR makers

Authors: Lutvija Karić, Maryam Golzardi, Petar Glamočlija, Jasmin Šutković

The five most common cultivars from the genus Allium cepa L. in Bosnia and Herzegovina (BiH) were analysed, focusing on Konjic onion. Seven SSR markers for genetic similarity analysis were used to address the genetic backgrounds. The polymorphic relationship between the SSR markers was analyzed by using Polymorphic Information Content (PIC) and Heterozygosity (H) b.. Read More»

Genet. Mol. Res. 17(1): gmr16039870

DOI: 10.4238/gmr16039870

Plant Genetics   Research Article

Molecular Characterization and Differential Interaction of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans with Common Beans (Phaseolus vulgaris)

Authors: De Paiva BAR, Wendland A , Borba TC , Araújo LG

The common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Xap) and X. fuscans subsp. fuscans (Xff), is one of the most important dry bean diseases of bacterial etiology. The present work aimed to study the genetic structure among and within populations, and also identify the pathogenic variability of Xap and Xff isolates evaluating the horizontal a.. Read More»

Genet. Mol. Res. 17(1): gmr16039871

DOI: 10.4238/gmr16039871

Plant Genetics   Review Article

Hierarchical outcrossing among and within fruits in Bertholletia excelsa Bonpl. (Lecythidaceae) open-pollinated seeds

Authors: L.D. Giustina, A.B. Baldoni, H. Tonini, V.C.R. Azevedo, L.G. Neves, F.D. Tardin, A.M. Sebbenn

Knowing the mating patterns is important to determine the number of trees necessary for seed collection for conservation ex situ, tree breeding and environmental reforestation purposes. We investigated B. excelsa individuals and fruits, to check mating system index variations in a population by using open-pollinated seeds which were hierarchically sampled within and.. Read More»

Genet. Mol. Res. 17(1): gmr16039872

DOI: 10.4238/gmr16039872

Medical Genetics   Research Article

Anthocyanins-loaded Eudragit® L100 nanoparticles: in vitro cytotoxic and genotoxic analysis

Authors: TB de Queiroz, D Dupeyrón, JCT Carvalho, IOM Gaivão, and EL Maistro

Anthocyanins are flavonoids compounds that have a great therapeutic potential acting as antioxidants, anti-inflammatory and anti-carcinogenic agents. They are consumed through food, pharmaceutical and cosmetics industries as a natural alternative to synthetic dyes. However, anthocyanins are unstable depending on pH, temperature, light and oxygen variations, which le.. Read More»

Genet. Mol. Res. 17(1): gmr16039875

DOI: 10.4238/gmr16039875

Human Genetics   Short communication

Role of KRAS in NSCLC: present and future

Authors: Addeo A, Bini R

Lung cancer is the leading cause of cancer-related deaths worldwide. Despite therapeutic advances over the last several decades, the overall 5-year survival remains only 16%. (Siegel R, 2013). Molecular studies have indicated that Adenocarcinomas have distinct genomic alterations allowing classification into clinically relevant molecular subsets. Such specific molec.. Read More»

Genet. Mol. Res. 17(1): gmr16039873

DOI: 10.4238/gmr16039873

Plant Genetics   Research Article

New insights into the genetic diversity and species identification of the native apricots in Southern Xinjiang of China

Authors: Ling Guo, Hui Li, Zheng-rong Luoa

Apricot is a staple stone fruit crop cultivated in Southern Xinjiang of China. This crop is important for the rural communities, as they generate significant employment and income. Here, seventy-eight apricot genotypes, including seventy-six common apricots (Prunus armeniaca L.) and two purple apricots (Prunus dasycarpa Ehrh.), were mainly collected from Aksu, Kashg.. Read More»

Genet. Mol. Res. 17(1): gmr16039874

DOI: 10.4238/gmr16039874

Plant Genetics   Research Article

Characterization of gametophytic self-incompatibility of superior clones of Coffea canephora

Authors: M.S. Moraes, A.L. Teixeira, A.R. Ramalho, M.C. Espíndula, M.A.G Ferrão and R.B. Rocha

The ability to avoid self-pollination is a trait that evolved as a manner of reducing the deleterious effects of inbreeding in various allogamous plant species, including Coffea canephora. The aim of this study was to perform directed hybridizations to characterize the compatibility groups of superior C. canephora clones, since plant selection can reduce variability.. Read More»

Genet. Mol. Res. 17(1): gmr16039876

DOI: 10.4238/gmr16039876

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