Table of contents: 2017
Animal Genetics   Research Article

Comparison of three methods for diagnosis of Trypanosoma (Duttonella) vivax in cattle

Authors: William Pereira Alves, Diana Abrão Cuglovici, Luis Fernando Viana Furtado,Julia Angélica Gonçalves da Silveira, Elias Jorge Facury-Filho,Múcio Flávio Barbosa Ribeiro and Élida Mara Leite Rabelo

Detection of Trypanosoma vivax in cattle is based on parasitological, serological and molecular methods. The aim of this study was to compare three different methods for detecting T. vivax during an outbreak of trypanosomiasis in southeast Brazil. Blood samples were collected from seventy-two animals to perform micro-hematocrit centrifugation technique (MHCT), indir.. Read More»

Genet. Mol. Res. 16(4): gmr16039811

DOI: 10.4238/gmr16039811

Microbial Genetics   Research Article

Development of 21 novel polynucleotide microsatellite markers in a Clithon retropictum (Gastropoda: Neritidae), using next-generation sequencing

Authors: Young Se Hyun, Ha Yeun Song, Min-Seop Kim, Hye Suck An

The neritid species Clithon retropictum (Gastropoda), an oviparous snail, is distributed in freshwater and brackish water in southern Korea, south-east Japan, southern China and Taiwan. This species is listed as a class II endangered species in Korea because of its decreasing population size. It has led to an increase in the need for genetic information about this s.. Read More»

Genet. Mol. Res. 16(4): gmr16039806

DOI: 10.4238/gmr16039806

Human Genetics   Research Article

IGF2BP2 gene polymorphism in the pathogenesis of vitiligo

Authors: Kursat Kargun, Betul Demir, Demet Cicek, Ozge Sevil Karstarli

Vitiligo is a disorder of pigmentation presenting with loss of melanocytes in the epidermis and hair follicles. The gene for insulin-like growth factor binding protein 2 (IGF2BP2) is located on the third chromosome and it plays role in growth, development, cellular differentiation, and metabolism. In the present study, we aimed to investigate the rs1470579 and rs440.. Read More»

Genet. Mol. Res. 16(4): gmr16039808

DOI: 10.4238/gmr16039808

Human Genetics   Research Article

AluYb8 insertion in the WNK1 gene is not associated with hypertension in a Russian Caucasian population

Authors: Elmira Akhmedova, Svetlana Y. Nikulina, Alla B. Salmina, Anna Chernova, Marina Bazanova, Anna Ohapkina, Aleksey Semenchukov, Paolo E. Maltese, Elena Manara and Matteo Bertelli

WNK1 (With No-lysine Kinase 1), a serine-threonine kinase, regulates blood pressure by acting on various sodium transport-related ion channels. Several studies report a link between common variants of the WNK1 gene and hypertension. No data exists on Russian populations. Our aim was to evaluate the association between the WNK1 AluYb8 polymorphism and hypertension su.. Read More»

Genet. Mol. Res. 16(4): gmr16039809

DOI: 10.4238/gmr16039809

Human Genetics   Research Article

Factor V Leiden G1691A and Prothrombin G20210A mutations are associated with repeated spontaneous miscarriage in Northern area of Saudi Arabia

Authors: Fakhr-Eldeen, A. Badawy, B. Abu AlSel and M. S. Fawzy

Maternally inherited thrombophilia could be one of the causes of recurrent spontaneous miscarriage (RSM). We aimed to investigate the frequency of three thrombotic gene variants; factor V Leiden (FVL; G1691A), prothrombin (PTH; G20210A), and methylenetetrahydrofolate reductase (MTHFR; C677T) in Saudi patients diagnosed with RSM. A case control study was conducted on.. Read More»

Genet. Mol. Res. 16(4): gmr16039810

DOI: 10.4238/gmr16039810

Plant Genetics   Research Article

Genome-Wide Analysis of a Radiation-Induced Lesion Mimic Mutant in Rice

Authors: Zhuo Zhenga, Mingcheng Guoa,b, Lihua Wua, Huimin Suna

Lesion mimic mutations (LMMs) are very useful genetic tools in plants, however, it is difficult to uncover genome-wide mutations using traditional technology, the development of whole genome sequencing (WGS) provides a means by which to identify genome-wide LMMs. In this study, WGS was performed to reveal the molecular mechanisms behind LMMs in rice. An LMM JA001 wa.. Read More»

Genet. Mol. Res. 16(4): gmr16039812

DOI: 10.4238/gmr16039812

Plant Genetics   Research Article

Genetic Variability for Sprout Growth among Genotypes of Coffea canephora Led by Bending of Orthotropic Stems

Authors: W.N. Rodrigues, T.V. Colodetti, S.V.B. Brinate, L.D. Martins and M. A. Tomaz

The multi-stem aspect of crop systems using Coffea canephora makes it necessary to correctly establish the number of orthotropic stems per plant during the crop formation. The present study was developed to study the variability of responses among improved genotypes of C. canephora to the technique of bending orthotropic stems as a mean of promoting the sprout growt.. Read More»

Genet. Mol. Res. 16(4): gmr16039813

DOI: 10.4238/gmr16039813

Plant Genetics   Research Article

Physiological Traits and Their Relationships in Black Oat Populations

Authors: D. Meira, C. Meier, T. Olivoto, M. Nardino, A. Rigatti, A. Lunkes, R. Bordin, V.S. Marchioro and V.Q. Souza

The objective of this work was to verify physiological quality of black oat populations, in order to know and point out the relationships among physiological characters for selection of progenies. The experiment was carried out at the Laboratory of Plant Breeding and Production, Federal University of Santa Maria, Campus of Frederico Westphalen - RS. Seed samples wer.. Read More»

Genet. Mol. Res. 16(4): gmr16039814

DOI: 10.4238/gmr16039814

Plant Genetics   Research Article

Characterization of the complete Chloroplast genome of Correa carmen, a valuable winter-flowering shrub

Authors: Minghui Chen, Yuxia Zong, Shiping Cheng, Zhilu Zhang

Correa carmen is considered important because of its considerable ornamental and economic value. The most striking characteristic of C. carmen flowers is their long winter-flowering period. In the present study, we generated the first complete C. carmen chloroplast genome sequence based on Illumina paired-end sequencing data. The entire chloroplast genome comprises .. Read More»

Genet. Mol. Res. 16(4): gmr16039815

DOI: 10.4238/gmr16039815

Human Genetics   Research Article

Comparative Study of Blood-Derived Scaffolds for the Culture of Human Adipose Derived Stem Cells (ASCS) and Dermal Fibroblasts

Authors: Helga Caputo Nunes, Rosana Rossi Ferreira,Ana Carolina Picolo Pasian, Andrei Moroz, Bruno Martinucci, Michele Janegitz Acorci Valerio,Sérgio Alexandre Alcântara dos Santos, Henrique de Souza Vieira,Sérgio Luis Felisbino, Elenice Deffune, Flávia Karina Delella

This study aimed to compare the performance of bloodderived scaffolds with a well-known and accepted scaffold, chitosan, in maintaining cell cultures of ASCs and fibroblasts for future wound healing applications. Cells were characterized, immunophenotyped and cultivated into the following scaffolds: 1) Chitosan (CH, control), 2) Platelet gel (PG), 3) Chitosan blende.. Read More»

Genet. Mol. Res. 16(4): gmr16039807

DOI: 10.4238/gmr16039807

Human Genetics   Research Article

DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer

Authors: T.D. Silva, A.V. Felipe, V.M. Vidigal, S.S. Saad, N.M. Forones

Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissues from patients with colorectal cancer .. Read More»

Genet. Mol. Res. 16(4): gmr16039816

DOI: 10.4238/gmr16039816

Plant Genetics   Research Article

Genetics Analysis of the Biggest Cashew Tree in the World

Authors: F.P.M. do Amaral, G.H. de Sá, L.A. Filgueiras, F.S. Santos Filho, C.J.R. Santos Soares, M.P.M. do Amaral, S.E. dos Santos Valente, A. N. Mendes

This study performed a genetic study of several canopies of Anacardium occidentale L. specimen called "Cashew King" (Cajueiro da Praia-PI, Brazil) in order to assess whether all canopies belong to the same plant. Leaves were collected from  different distant points, and preserved for analysis of DNA. The analysis was made from automated sequencing by .. Read More»

Genet. Mol. Res. 16(4): gmr16039817

DOI: 10.4238/gmr16039817

Human Genetics   Research Article

Promotion of apoptosis in high glucose-activated hepatic stellate cells by GLP-1 receptor agonist and its potential mechanism

Authors: Lingkang Wu, Yingchao Liu, Liangliang Shi, Bingbing Zhang, Ana Carolina Picolo Pasian and Andrei Moroz

This study aimed to investigate the stimulatory effect of glucagon-like peptide 1 (GLP-1) receptor agonist (GLP-1RA) on the apoptosis of hepatic stellate cells (HSCs) activated by high glucose, and to explore the underlying molecular mechanism with a focus on the c-Jun N-terminal kinase (JNK) and extracellular signal-related kinase (ERK) signaling pathways. Human HS.. Read More»

Genet. Mol. Res. 16(4): gmr16039818

DOI: 10.4238/gmr16039818

Animal Genetics   Research Article

BmICE-2 interference vector construction and its silencing effect in the BmN-SWU1 cells

Authors: Hua-Shan Yi and Xianping Ma

Investigate the function of BmICE-2 gene in silkworm cells by constructing a BmICE-2 interference vector and analysing its interference effect. Online RNAi design software was used to predict the interference target sequence of Bmice-2. An optimized sequence was selected and inserted into the backbone of interference microRNA. The backbone of interference vector, mi.. Read More»

Genet. Mol. Res. 16(4): gmr16039819

DOI: 10.4238/gmr16039819

Plant Genetics   Research Article

Differential expression of dormancy-Associated genes in fastigiata and hypogaea peanut

Authors: M.F.C. Silva, C.R.C. Silva, L.M. Lima and R.C. Santos

Seed dormancy is a temporary replacement state observed in some higher plants, which prevents germination under unfavorable conditions. In several species, dormancy process is triggered by hormonal factors, initiated by ABA and precursor enzymes. In this work, we investigated the relationship between dormancy and the expression of ARP, DMR1, and NCED genes in uprigh.. Read More»

Genet. Mol. Res. 16(4): gmr16039820

DOI: 10.4238/gmr16039820

Human Genetics   Research Article

Genotypes within the prolactin and growth hormone insulin-like growth factor-I pathways associated with milk production in heat stressed Holstein cattle: Genotypes and milk yield in heat stressed Holstein cows

Authors: Hernández-Cordero, M. A. Sánchez-Castro, R. Zamorano- Algandar, P. Luna-Nevárez, G. Rincón, J. F. Medrano4, S. E. Speidel, R. M. Enns and M. G. Thomas

The association of single nucleotide polymorphisms (SNPs) within genes of the prolactin (PRL), growth hormone (GH) and insulin-like growth factor-I (IGF1) pathways with 305-day milk yield (MY305) were evaluated in heat-stressed Holstein cows. Ambient temperature and relative humidity were used to calculate a temperature-humidity index (THI), which revealed that heat.. Read More»

Genet. Mol. Res. 16(4): gmr16039821

DOI: 10.4238/gmr16039821

Human Genetics   Research Article

Case report of X-linked hyper-IgM syndrome with acquired toxoplasmic encephalitis

Authors: Guoyan Lu, Dan Yu

The X-linked hyper-IgM (XHIGM; HIGM1) syndrome is an uncommon primary immunodeficiency disease caused by mutations in the gene for CD40 ligand and characterized by normal or elevated serum IgM, low or absent serum levels of IgG, IgA and IgE, and defective T-cell function. Here we reported a case of XHIGM with acquired toxoplasmic encephalitis. The patient experience.. Read More»

Genet. Mol. Res. 16(4): gmr16039822

DOI: 10.4238/gmr16039822

Human Genetics   Research Article

Clinical and molecular findings in an Albanian family with familial adenomatous polyposis

Authors: P. E. Maltese, G. Di Saverio, E. Manara, F. Fanelli, N. Capodicasa, D. Guraj, I. Shehaj, B. Amato, A. Babameto-Laku, S. Michelini , M. Bertelli

Purpose: Familial adenomatous polyposis is an inherited precancerous condition characterized by multiple colorectal polyps. This brief report describes three generations of a family with a history of colorectal cancer in which genetic testing was useful for detecting individuals at risk. Methods: A next generation sequencing custom gene panel comprising 11 genes ass.. Read More»

Genet. Mol. Res. 16(4): gmr16039823

DOI: 10.4238/gmr16039823

Animal Genetics   Research Article

Epidemiological and pathological studies on the helminthic parasites in native chickens of Tabriz city, Iran

Authors: Garedaghi yagoob, Arash Khaki, Sayed Haidar Abbas Raza,Rajwali Khan, Li Hao, Seyed Mahdi Hosseini, Sherein S. Abdelgayed and Mohib Ullah Kakar

A study of intestinal helminthic parasites of domestic chickens was carried out in Tabriz city, Iran during 2016. One hundred (100) gastrointestinal samples were examined in necropsied chickens microscopically using the lactophenol and carmine-acid staining technique. The result showed a high prevalence of intestinal parasitic infection. Out of 100 domestic chicken,.. Read More»

Genet. Mol. Res. 16(4): gmr16039824

DOI: 10.4238/gmr16039824

Animal Genetics   Research Article

Transferability of heterologous microsatellite loci between species of Euterpe genus

Authors: H.S.F.S Azêvedo, F.D Benvindo, L.N. Cavalcante, M. Haverroth, L.H.O. Wadt, T. Campos

Euterpe precatoria, popularly known as “açaí-do-amazonas”, has agronomic, technological and economic potential and has been gaining prominence with the commercialization of its fruits. The production of this açaí-do-amazonas is based on extractivism and management practices have been recommended for sustainable collection. To .. Read More»

Genet. Mol. Res. 16(4): gmr16039825

DOI: 10.4238/gmr16039825

Human Genetics   Research Article

Mitochondrial diversity in the populations of Pediculus humanus capitis across eastern south of Tehran Province, Iran

Authors: Fatemeh Ghandali, Neda Kheradpir, Siamak Yousefi Siahkalroudi

Pediculus humanus capitis is a major obligate and district ecto-parasite of human which is distributed in different parts of the world and is under several research projects for population management and molecular analysis. In this study, one hundred and seventy head lice were collected from school girls at different high schools in five cities in south east of Tehr.. Read More»

Genet. Mol. Res. 16(4): gmr16039826

DOI: 10.4238/gmr16039826

Human Genetics   Research Article

Promotion of apoptosis in high glucose-activated hepatic stellate cells by GLP-1 receptor agonist and its potential mechanism.

Authors: Lingkang Wu, Yingchao Liu, Liangliang Shi, Bingbing Zhang, Ana Carolina Picolo Pasian and Andrei Moroz

This study aimed to investigate the stimulatory effect of glucagon-like peptide 1 (GLP-1) receptor agonist (GLP-1RA) on the apoptosis of hepatic stellate cells (HSCs) activated by high glucose, and to explore the underlying molecular mechanism with a focus on the c-Jun N-terminal kinase (JNK) and extracellular signal-related kinase (ERK) signaling pathways. Human HS.. Read More»

Genet. Mol. Res. 16(4): gmr16039818

DOI: 10.4238/gmr16039818

Microbial Genetics   Research Article

Screening of peste des petits ruminants virus in a population of district Khairpur, Pakistan

Authors: A.K. Maitlo, J.A. Ujan, S.A. Ujjan, M. Ruk, B.A. Memon, A.A. Mahar and A.A. Ujjan

Goats are the Pakistan’s fastest growing ruminants, and Pakistan is the third largest goat producer in the world after India and China. Goat meat preference is the main reason for its increased demand. In the country, there are 25 goat breeds and two wild relatives such as Mark and Goats. At present, Pakistan has 53.8 million goats, according to the 2006 GOP r.. Read More»

Genet. Mol. Res. 16(3): gmr16039679

DOI: 10.4238/gmr16039679

Plant Genetics   Research Article

Genotypic variation and relationships among nitrogen-use efficiency and agronomic traits in tropical maize inbred lines

Authors: M.C. Rodrigues, W.M. Rezende, M.E.J. Silva, S.V. Faria, L.T. Zuffo, J.C.C. Galvão and R.O. DeLima

Improvement in nitrogen-use efficiency (NUE) on maize is among the best strategies to mitigate the problems of poor soil fertility in tropical conditions. The objectives of this study were: i) to quantify the genetic variability for NUE-components and agronomic traits in a set of tropical maize inbred lines; ii) to study the genetic divergence among tropical maize i.. Read More»

Genet. Mol. Res. 16(3): gmr16039757

DOI: 10.4238/gmr16039757

Microbial Genetics   Research Article

Evaluation of genome similarities using the non-decimated wavelet transform

Authors: L.M. Ferreira, T. Sáfadi and R.R. Lima

The wavelets have become increasingly popular in the field of bioinformatics due to their capacity in multiresolution analysis and space-frequency localization; the latter particularity is acquired due to a moving window that runs through the analyzed space. As a feature, they have a better ability to capture hidden components of biological data and an efficient lin.. Read More»

Genet. Mol. Res. 16(3): gmr16039758

DOI: 10.4238/gmr16039758

Animal Genetics   Research Article

Study of genetic variability in pigs after the traditional breeding program

Authors: I.M. Ferreira, G.S. Vieira, T.F. Braga, T.C.F. Silva, M.M. Franco and R.C. Antunes

Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency of single nucleotide polymorphism was pe.. Read More»

Genet. Mol. Res. 16(3):

Plant Genetics   Research Article

Performance of grain sorghum hybrids under drought stress using GGE biplot analyses

Authors: P.S.C. Batista, C.B. Menezes, A.J. Carvalho, A.F. Portugal, E.A. Bastos, M.J. Cardoso, C.V. Santos and M.P.M. Julio Centro de

The objective of this study was to estimate the adaptability and stability of grain sorghum hybrids grown under post-flowering water stress and non-stress conditions. The trials were carried out in Nova Porteirinha-MG during the season of 2014 and 2015, and in Teresina-PI in the 2014 season. Twenty-nine-grain sorghum hybrids were evaluated, in a randomized complete .. Read More»

Genet. Mol. Res. 16(3): gmr16039761

DOI: 10.4238/gmr16039761

Human Genetics   Research Article

Association between gastrointestinal cancers and fingerprint patterns in the Iranian population

Authors: S. Abbasi and M. Rasouli

Gastrointestinal cancers are malignant diseases with high mortality rate. Early diagnosis of patients could improve the results of treatment. Many studies used dermatoglyphics as a biomarker to predict the incidence of genetic diseases and cancers. This study assessed the association between gastrointestinal cancers and particular fingerprint patterns, which could b.. Read More»

Genet. Mol. Res. 16(3): gmr16039762

DOI: 10.4238/gmr16039762

Plant Genetics   Research Article

Mating system and intrapopulational genetic diversity of Copernicia prunifera (Arecaceae): a native palm from Brazilian semiarid

Authors: R.A.R. Silva, C.G. Fajardo and F.A. Vieira

The aim of this study was to investigate the mating system and genetic diversity of the palm Copernicia prunifera using inter-simple sequence repeat markers. We found that the C. prunifera has multiple inflorescences with hermaphroditic flowers and pollen viability of 62%. Outcrossing rates at the population level (N = 267) produced a multilocus outcrossing rate (tm.. Read More»

Genet. Mol. Res. 16(3): gmr16039764

DOI: 10.4238/gmr16039764

Plant Genetics   Research Article

Fertility restoration locus and cytoplasm types in onion

Authors: R.R. Ferreira, C.A.F. Santos and V.R. Oliveira

The objective of this study was the identification of the cytoplasmic types and the genotyping for the fertility restoration nuclear locus (Ms) in 59 onion accessions, aiming at the selection of ‘A’ and ‘B’ lines essential for the obtainment of hybrids. Three markers were used to identify the cytoplasm 5’ cob, orfA501, and orf725, and t.. Read More»

Genet. Mol. Res. 16(3): gmr16039766

DOI: 10.4238/gmr16039766

Plant Genetics   Research Article

Genetic diversity in soybean genotypes using phenotypic characters and enzymatic markers

Authors: E.V. Zambiazzi, A.T. Bruzi, A.P. Sales, I.M.M. Borges, S.R. Guilherme, A.M. Zuffo, J.G. Lima, F.O. Ribeiro, A.E.S. Mendes, S.H.M Godinho and M.L.M. Carvalho

The objective of this study was to evaluate the genetic diversity of soybean cultivars by adopting phenotypic traits and enzymatic markers, the relative contribution of agronomic traits to diversity, as well as diversity between the level of technology used in soybean cultivars and genetic breeding programs in which cultivars were inserted. The experiments were cond.. Read More»

Genet. Mol. Res. 16(3): gmr16039770

DOI: 10.4238/gmr16039770

Plant Genetics   Research Article

Genetic diversity of Schizolobium parahyba var. amazonicum (Huber ex. Ducke) Barneby, in a forest area in Brazil

Authors: A.L. Silva Júnior, L.C. Souza, A.G. Pereira, M.V.W. Caldeira and F.D. Miranda

Schizolobium parahyba var. amazonicum (Fabaceae) is an arboreal species, endemic to the Amazon Rainforest, popularly known as paricá. It is used on a commercial scale in the timber sector, pulp and paper production, reclamation projects in degraded and landscaped areas. However, there is no availability of genetically improved material selected for the enviro.. Read More»

Genet. Mol. Res. 16(3): gmr16039774

DOI: 10.4238/gmr16039774

Plant Genetics   Research Article

Genetic diversity of Plathymenia reticulata Benth. in fragments of Atlantic Forest in southeastern Brazil

Authors: L.C. Souza, A.L. Silva Júnior, M.C. Souza, S.H. Kunz and F.D. Miranda

Studies of genetic diversity in natural populations are important for the definition of conservation strategies, especially in populations reduced by processes of fragmentation and continuous forest extraction. Molecular markers stand out as interesting tools for these studies. The objective of this research was to characterize the diversity and genetic structure of.. Read More»

Genet. Mol. Res. 16(3): gmr16039775

DOI: 10.4238/gmr16039775

Human Genetics   Research Article

Ewes carrying the Booroola and Vacaria prolificacy alleles respond differently to ovulation induction with equine chorionic gonadotrophin

Authors: J.C.F. Moraes and C.J.H. Souza

The magnitude of ovulation rate (OR) after hormonal induction in sheep should be considered when prolific genotypes are used. We investigated for the first time the effect of the Vacaria allele and its combined effect with the Booroola prolificacy mutation on OR after hormonal treatment during breeding and anoestrous season. A hundred forty-nine Ile de France crossb.. Read More»

Genet. Mol. Res. 16(3): gmr16039787

DOI: 10.4238/gmr16039787

Plant Genetics   Research Article

Genetic diversity of Saccharum complex using ISSR markers

Authors: L.A.R. Oliveira, C.A. Machado, M.N. Cardoso, A.C.A. Oliveira, A.L. Amaral, A.R.C. Rabbani, A.V.C. Silva and A.S. Ledo

Sugarcane (Saccharum sp, Poaceae) is native to Southeast Asia, and due to growing demand as raw material, its cultivation recently expanded to new frontiers. The genetic diversity analysis is essential for targeting strategies in the formation and maintenance of a germplasm. This study aimed to assess the genetic diversity of 26 accessions of sugarcane from the Acti.. Read More»

Genet. Mol. Res. 16(3): gmr16039788

DOI: 10.4238/gmr16039788

Plant Genetics   Research Article

Periclinal chimera technique: new plant breeding approach

Authors: P.M. Gakpetor, H. Mohammed, D. Moreti and N.M.A. Nassar

Plant interspecific periclinal chimeras are a mosaic formed by tissues from two species. They are manipulated here as an efficient plant breeding tool for cassava root yields. In this study, plants synthesized from two chimeras, designated as chimera 2 and chimera 4, were characterized morphologically and cytologically to unravel the origin of their tissue layers (L.. Read More»

Genet. Mol. Res. 16(3): gmr16039790

DOI: 10.4238/gmr16039790

Plant Genetics   Research Article

Genetic diversity of high performance cultivars of upland and irrigated Brazilian rice.

Authors: G.R.C. Coelho, C. Brondani, L.V. Hoffmann, P.A.M.R. Valdisser, T.C.O. Borba, J.A. Mendonça, L.A. Rodrigues and I.P.P. de Menezes

The objective of this study was to analyze the diversity and discrimination of high-performance Brazilian rice cultivars using microsatellite markers. Twenty-nine rice cultivars belonging to EMBRAPA Arroz e Feijão germplasm bank in Brazil were genotyped by 24 SSR markers to establish their structure and genetic discrimination. It was demonstrated that the ana.. Read More»

Genet. Mol. Res. 16(3): gmr16039793

DOI: 10.4238/gmr16039793

Plant Genetics   Research Article

Genetic diversity among 16 genotypes of Coffea arabica in the Brazilian cerrado

Authors: C.M.S. Machado, N.S. Pimentel, A. Golynsk, A. Ferreira, H.D. Vieira and F.L. Partelli

For the selection of coffee plants that have favorable characteristics, it is necessary to evaluate variables related to production. Knowledge of the genetic divergence of arabica coffee is of extreme importance, as this knowledge can be associated with plant breeding programs in order to combine genetic divergence with good productive performance. The objective of .. Read More»

Genet. Mol. Res. 16(3): gmr16039794

DOI: 10.4238/gmr16039794

Human Genetics   Research Article

Improving the PCR protocol to amplify a repetitive DNA sequence

Authors: J. Riet, L.R.V. Ramos, R.V. Lewis and L.F. Marins

Although PCR-based techniques have become an essential tool in the field of molecular and genetic research, the amplification of repetitive DNA sequences is limited. This is due to the truncated nature of the amplified sequences, which are also prone to errors during DNA polymerase-based amplification. The complex structure of repetitive DNA can form hairpin loops, which promote dissociation of.. Read More»

Genet. Mol. Res. 16(3): gmr16039796

DOI: 10.4238/gmr16039796

Microbial Genetics   Research Article

In silico modeling and characterization of phytoparasitic nematodes translationally-controlled tumor proteins

Authors: R.M. Moraes Filho, A.F. Menezes and L.S.S. Martins

Plant parasitic nematodes infect a wide range of hosts representing the largest source of biotic stress experienced by plants. Meloidogyne genus comprises the most important parasitic nematodes, also known as root-knot nematodes. These parasitic organisms obtain nutrients to support their development through complex interactions with their hosts. The translationally.. Read More»

Genet. Mol. Res. 16(3): gmr16039800

DOI: 10.4238/gmr16039800

Plant Genetics   Research Article

Genetic control of number of flowers and pod set in common bean

Authors: E.S. Martins, R.A. Pinto Júnior, A.F.B. Abreu and M.A.P. Ramalho

This article aimed to study the genetic control of some flowers and pod set of common bean and to verify if its estimate varies with environmental conditions and gene pool. A complete diallel was used among six lines, but no reciprocal ones. The treatments were evaluated in three harvests/generations - F2, F3, and F4 - in 2015/2016, in a randomized complete block de.. Read More»

Genet. Mol. Res. 16(3): gmr16039723

DOI: 10.4238/gmr16039723

Animal Genetics   Research Article

Tissue distribution and early developmental expression patterns of aldolase A, B, and C in grass carp Ctenopharyngodon idellus

Authors: J.J. Fan, J.J. Bai, D.M. Ma, L.Y. Yu and P. Jiang

Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model ow.. Read More»

Genet. Mol. Res. 16(3): gmr16039234

DOI: 10.4238/gmr16039234

Plant Genetics   Review Article

Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding

Authors: M.P. Lavinscky, M.M. Souza, G.S. Silva and C.A.F. Melo

The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may lead to complete sterility of the plant.. Read More»

Genet. Mol. Res. 16(3): gmr16039582

DOI: 10.4238/gmr16039582

Animal Genetics   Research Article

Identification of differentially expressed genes in pathways of cerebral neurotransmission of anovulatory mice

Authors: M.A. Azevedo Jr. and I.D.C.G. Silva

Polycystic ovary syndrome is the classic example of loss of functional cyclicity and anomalous feedback. In this case, the excessive extra-glandular production and conversion of androgens to estrogens are the pathophysiological basis of the chronic anovulation. The literature describes an experimental model of the polymicrocystic ovary in obese diabetic mice with in.. Read More»

Genet. Mol. Res. 16(3): gmr16039622

DOI: 10.4238/gmr16039622

Plant Genetics   Research Article

Genetic diversity of arboreal cotton populations of the Brazilian semiarid: a remnant primary gene pool for cotton cultivars

Authors: I.P.P. de Menezes, L.V. Hoffmann, T.H. de Lima, A.R. da Silva, V.S. Lucena and P.A.V. Barroso

Mocó cotton belong to the same species as the cultivated species, Gossypium hirsutum, and cultivated forms were mainly landraces but also developed as cultivars, bearing good fiber quality and drought tolerant when cropped as a perennial species. The northeast Brazil crop system based on this cotton type is finished, with a few small area planted in the three.. Read More»

Genet. Mol. Res. 16(3): gmr16039659

DOI: 10.4238/gmr16039659

Plant Genetics   Research Article

Intraspecific variability of popcorn S7 lines for phosphorus efficiency in the soil

Authors: I.F.S. Gerhardt, A.T. Amaral Júnior, L.J.M. Guimarães, I.A. Schwantes, A. Santos, S.H. Kamphorst, V.J. Lima, F.M. Poblete and G.O. Myers

The expansion of agriculture, coupled with the need for sustainable cropping, is one of the greatest challenges of the scientific community working on the generation of new cultivars adapted to abiotic stress conditions. The aim of this study was to evaluate the variability of popcorn lines as to responsiveness and efficiency in phosphorus use, as a first step towar.. Read More»

Genet. Mol. Res. 16(3):

Animal Genetics   Research Article

Analysis of melanopsin gene expression in the rabbit retina at different ages

Authors: M.M. Lira-Carrera, B.E. Gutiérrez-Amavizca, L.J. Álvarez-Araujo, M. Aguirre-Ramírez and J.A. Pérez-León

Melanopsin is the photopigment of intrinsically photosensitive retinal ganglion cells that mediate non-visual responses to light. The aim of this study was to describe and analyze melanopsin gene expression in the rabbit retina at different ages and compare its expression with the prototypic gene of retinal ganglion cells (Thy-1 gene). Expression levels of OPN4, Thy.. Read More»

Genet. Mol. Res. 16(3): gmr16039767

DOI: 10.4238/gmr16039767

Plant Genetics   Research Article

Anthropization as a determinant factor in the genetic structure of Copernicia prunifera (Arecaceae)

Authors: L.G. Pinheiro, K.P.T. Chagas, A.S.M. Freire, M.C. Ferreira,C.G. Fajardo and F.A. Vieira

This study uses ISSR molecular markers to characterize the demographic pattern, and spatial genetic structure (SGS) at different life stages of development (cohorts) in a natural population of Copernicia prunifera in the Rio Grande do Norte State, Brazil. All individuals were sampled and georeferenced in a 0.55-ha plot. The demographic analyses showed a clustered pa.. Read More»

Genet. Mol. Res. 16(3): gmr16039768

DOI: 10.4238/gmr16039768

Plant Genetics   Research Article

Indices estimated using REML/BLUP and introduction of a super-trait for the selection of progenies in popcorn

Authors: C. Vittorazzi, A.T. Amaral Junior, A.G. Guimarães, A.P. Viana, F.H.L. Silva, G.F. Pena, R.F. Daher, I.F.S Gerhardt, G.H.F. Oliveira and M.G. Pereira

Selection indices commonly utilize economic weights, which become arbitrary genetic gains. In popcorn, this is even more evident due to the negative correlation between the main characteristics of economic importance - grain yield and popping expansion. As an option in the use of classical biometrics as a selection index, the optimal procedure restricted maximum lik.. Read More»

Genet. Mol. Res. 16(3): gmr16039769

DOI: 10.4238/gmr16039769

Microbial Genetics   Research Article

Genetic diversity of Bacillus sp producers of amylase isolated from the soil

Authors: A.R.E.O. Xavier, E.R. Lima, A.M.E. Oliveira, L. Cardoso, J. Santos, C.H.C. Cangussu, L.N. Leite, M.C.L. Quirino, I.G.C. Júnior,D.A. Oliveira and M.A.S. Xavier

The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective of this study was to isolate and analyz.. Read More»

Genet. Mol. Res. 16(3): gmr16039771

DOI: 10.4238/gmr16039771

Plant Genetics   Research Article

Genetic control and combining ability of agronomic attributes and northern leaf blight-related attributes in popcorn

Authors: J.S. Santos, A.T. Amaral Júnior, M. Vivas, G.S. Mafra, G.F. Pena,F.H.L. Silva and A.G. Guimarães

The present study was conducted to investigate the genetic control and to estimate the general and specific combining abilities of popcorn for agronomic attributes and attributes related to resistance to northern leaf blight (NLB). The 56 hybrids (F1 and reciprocals), together with the eight parent lines and six controls, were evaluated in two harvests, in a randomi.. Read More»

Genet. Mol. Res. 16(3): gmr16039772

DOI: 10.4238/gmr16039772

Plant Genetics   Research Article

Agricultural performance and genetic parameters for yield-related traits of sugar- and energy cane families derived from planned crosses

Authors: A.R. Fernandes Júnior, A.A.C. de Azeredo, R.A. de Oliveira, J.C. Bespalhok Filho, O.T. Ido, E. Daros and B.P. Brasileiro

The forecast of a growing energy demand in the coming years has aroused particular interest in biomass for energy cogeneration, to diversify the energy matrix by using clean and renewable sources. To meet the new demands of the sugarcane industry, this study evaluated the agronomic performance and estimated genetic parameters for yield traits in sugar- and energy ca.. Read More»

Genet. Mol. Res. 16(3): gmr16039773

DOI: 10.4238/gmr16039773

Plant Genetics   Research Article

Genetic divergence in elite castor bean lineages based on TRAP markers

Authors: K.S. Simões, S.A. Silva, E.L. Machado and M.S. Silva

Castor bean (Ricinus communis L.) is a tropical plant of great commercial interest and a potential source of biodiesel. The development of genetically improved cultivars with high amounts of oil in the seeds and low ricin toxicity is crucial to increase the productivity of this crop. The use of TRAP (target region amplification polymorphism) markers to develop elite.. Read More»

Genet. Mol. Res. 16(3): gmr16039776

DOI: 10.4238/gmr16039776

Human Genetics   Research Article

Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2)

Authors: T.B. Queiroz, G.F. Santos, S.C. Ventura, C.A. Hiruma-Lima, I.O.M. Gaivão and E.L. Maistro

Geraniol is an acyclic monoterpene alcohol present in the essential oil of many aromatic plants and is one of the most frequently used molecules by the flavor and fragrance industries. The literature also reports its therapeutic potential, highlighting itself especially as a likely molecule for the development of drugs against cancer. In view of these considerations.. Read More»

Genet. Mol. Res. 16(3): gmr16039777

DOI: 10.4238/gmr16039777

Plant Genetics   Research Article

Structure and genetic diversity of Anacardium humile (Anacardiaceae): a tropical shrub

Authors: L.G. Cota, P.A. Moreira, M.M. Brandão, V.A. Royo, A.F. Melo Junior, E.V. Menezes and D.A. Oliveira

Anacardium humile Saint Hilaire is a tropical shrub native to the Cerrado biome. It is a fruiting species with biological, medicinal, and socioeconomic significance. Thus, knowing how the genetic variability of natural populations is organized allows for the establishment of strategies for conservation and the sustainable use of the species and its biome. Six micros.. Read More»

Genet. Mol. Res. 16(3): gmr16039778

DOI: 10.4238/gmr16039778

Human Genetics   Research Article

Assessment of the rs4340 ACE gene polymorphism in acute coronary syndrome in a Western Mexican population

Authors: A. Valdez-Haro, Y. Valle, E. Valdes-Alvarado, F. Casillas-Muñoz, J.F. Muñoz-Valle, G.L. Reynoso-Villalpando, H.E. Flores-Salinas and J.R. Padilla-Gutiérrez

Acute coronary syndrome (ACS) is considered one of the main causes of death worldwide. Contradictory findings concerning the impact of the angiotensin-converting enzyme (ACE) gene on cardiovascular diseases have been reported. Previous conclusions point out that the variability in results depends on ethnicity and genetic polymorphisms to determine the association of.. Read More»

Genet. Mol. Res. 16(3): gmr16039779

DOI: 10.4238/gmr16039779

Human Genetics   Case Reports

Down-Klinefelter syndrome (48,XXY,+21) in a neonate associated with congenital heart disease

Authors: M.A. Rodrigues, L.F. Morgade, L.F.A. Dias, R.V. Moreira, P.D. Maia, A.F.H. Sales and P.D. Ribeiro

Double aneuploidy is considered a rare phenomenon. Herein, we describe a case of double aneuploidy 48,XXY,+21 in a neonate with congenital heart defects. The 28-day-old neonate male (23-year-old mother and 24-year-old father) was admitted to a neonatal intensive care unit owing to congenital heart disease. Echocardiography showed a complete atrioventricular septal d.. Read More»

Genet. Mol. Res. 16(3): gmr16039780

DOI: 10.4238/gmr16039780

Plant Genetics   Research Article

Prediction of genetic gains by selection indices using mixed models in elephant grass for energy purposes

Authors: V.B. Silva, R F. Daher, M.S.B. Araújo, Y.P. Souza, S. Cassaro, B.R.S. Menezes, L.M. Gravina, A.A.C. Novo, F.D. Tardin and A.T. Amaral Júnior

Genetically improved cultivars of elephant grass need to be adapted to different ecosystems with a faster growth speed and lower seasonality of biomass production over the year. This study aimed to use selection indices using mixed models (REML/BLUP) for selecting families and progenies within full-sib families of elephant grass (Pennisetum purpureum) for biomass pr.. Read More»

Genet. Mol. Res. 16(3): gmr16039781

DOI: 10.4238/gmr16039781

Plant Genetics   Research Article

Chemotherapeutical effects of the herbal medicine Uncaria tomentosa (Willd.) DC.

Authors: I.V. Almeida, L.C. Soares, F.T. Lucio, L.B. Cantagalli, A.F. Reusing and V.E.P. Vicentini

The use of medicinal plants dates back to the beginning of humanity, and today their application as complementary therapy has been widely disseminated as an alternative to conventional therapy. The medicinal plant named Uncaria tomentosa (Willd.) DC. (known as cat’s claw) is a common woody vine of the Amazon forest that has traditionally been used in the treat.. Read More»

Genet. Mol. Res. 16(3): gmr16039782

DOI: 10.4238/gmr16039782

Plant Genetics   Research Article

In vivo antimutagenic activity of the medicinal plants Pfaffia glomerata (Brazilian ginseng) and Ginkgo biloba

Authors: I.V. Almeida, E. Düsman, G.I. Mattge, F. Toledo, A.F. Reusing and V.E.P. Vicentini

Complementary and alternative therapies, including the use of medicinal plants, have become almost standard among the world’s population. Pfaffia glomerata (PG), popularly known as Brazilian ginseng, is widely used as a restorer of vital functions, increasing mental balance, and is used for the treatment of diabetes and rheumatism. Ginkgo biloba (GB) is one of.. Read More»

Genet. Mol. Res. 16(3): gmr16039785

DOI: 10.4238/gmr16039785

Plant Genetics   Research Article

Identification of soybean genotypes with high stability for the Brazilian macro-region 402 via biplot analysis

Authors: E.U. Ramos Junior, R.L. Brogin, V.P.C. Godinho, F.J.E. Botelho, F.D. Tardin and P.E. Teodoro

Biplot analysis has often been used to recommend genotypes from different crops in the presence of the genotype x environment interaction (GxE). The objective of this study was to verify the association between the AMMI and GGE biplot methods and to select soybean genotypes that simultaneously meet high grain yield and stability to the environments belonging to the .. Read More»

Genet. Mol. Res. 16(3): gmr16039786

DOI: 10.4238/gmr16039786

Plant Genetics   Research Article

Multiple genetic resistances in Capsicum spp

Authors: C.S. Bento, A.G. de Souza, C.P. Sudré, S. Pimenta and R. Rodrigues

This study aimed to identify Capsicum genotypes with resistance to bacterial spot (BS), anthracnose and Pepper yellow mosaic virus (PepYMV). Fifty-four genotypes of Capsicum spp were evaluated. Resistance reaction against BS was evaluated using three replicates, testing hypersensitivity and quantitative resistance in leaves. After evaluation, inoculated leaves were .. Read More»

Genet. Mol. Res. 16(3): gmr16039789

DOI: 10.4238/gmr16039789

Plant Genetics   Research Article

Genotype x environment interaction and stability of soybean cultivars for vegetative-stage characters

Authors: M.V.A. Chaves, N.S. Silva, R.H.O. Silva, G.L. Jorge, I.C. Silveira, L.A. Medeiros, R.L. Hamawaki, O.T. Hamawaki, A.P.O. Nogueira and C.D.L. Hamawaki

In order to obtain the certificate of cultivar protection, it is necessary to prove its distinctiveness, homogeneity, and stability. Currently, there are 37 descriptors for differentiating soybeans cultivars. However, they are still not enough and, as a result, it is necessary to create, identify, and evaluate new descriptors. This study was aimed at evaluating the .. Read More»

Genet. Mol. Res. 16(3): gmr16039795

DOI: 10.4238/gmr16039795

Human Genetics   Research Article

Allele frequencies in Azuay Population in Ecuador

Authors: P.P. Orellana, C.F. Andrade, C.L. Arciniegas and G.C. Iannacone

One hundred and eighty-two samples of unrelated people who requested the paternity test at the Molecular Biology and Genetics Laboratory of the Catholic University of Cuenca-Ecuador in the province of Azuay were studied, except for the D1S1656 (180 samples) and SE33 (89 samples) markers. The STRs D22S1045, D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19.. Read More»

Genet. Mol. Res. 16(3): gmr16039797

DOI: 10.4238/gmr16039797

Plant Genetics   Research Article

Cotton genotypes selection through artificial neural networks

Authors: E.G. Silva Júnior, D.B.O. Cardoso, M.C. Reis, A.F.O. Nascimento, D.I. Bortolin, M.R. Martins and L.B. Sousa

Breeding programs currently use statistical analysis to assist in the identification of superior genotypes at various stages of a cultivar’s development. Differently from these analyses, the computational intelligence approach has been little explored in genetic improvement of cotton. Thus, this study was carried out with the objective of presenting the use of.. Read More»

Genet. Mol. Res. 16(3): gmr16039798

DOI: 10.4238/gmr16039798

Plant Genetics   Research Article

Molecular identification of variety purity in a cotton hybrid with unknown parentage using DNA-SSR markers

Authors: X.Q. Fu, F.X. Yang, X.K. Lu, X.G. Wang, B.X. Yang, F.J. Liu, Y. Liu and J. Peng

Molecular identification of hybrid purity is difficult in regional trials of cotton varieties and hybrid trials. In particular, the molecular detection of hybrid purity has not yet been reported in the case of unknown parentage. In this study, we screened 5000 pairs of primers and chose 17 pairs of core simple sequence repeat (SSR) primers to determine the F1 purity.. Read More»

Genet. Mol. Res. 16(3): gmr16039799

DOI: 10.4238/gmr16039799

Plant Genetics   Research Article

Estimates of genetics and phenotypics parameters for the yield and quality of soybean seeds

Authors: E.V. Zambiazzi, A.T. Bruzi, S.R. Guilherme, D.R. Pereira, J.G. Lima, A.M. Zuffo, F. O. Ribeiro, A.E.S. Mendes, S.H.M. Godinho and M.L.M. Carvalho

Estimating genotype x environment (GxE) parameters for quality and yield in soybean seed grown in different environments in Minas Gerais State was the goal of this study, as well as to evaluate interaction effects of GxE for soybean seeds yield and quality. Seeds were produced in three locations in Minas Gerais State (Lavras, Inconfidentes, and Patos de Minas) in 20.. Read More»

Genet. Mol. Res. 16(3): gmr16039801

DOI: 10.4238/gmr16039801

Animal Genetics   Research Article

Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid

Authors: P.A.S.V. Carvalho, A.C. Brasileiro, S. Leal-Bertioli, D.J. Bertioli, J.P. Silva, T.S. Agostini-Costa and M.A. Gimenes

Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase gene.. Read More»

Genet. Mol. Res. 16(3): gmr16039802

DOI: 10.4238/gmr16039802

Plant Genetics   Research Article

Diversity among elephant grass genotypes using Bayesian multi-trait model

Authors: D.A. Rossi, R.F. Daher, T.C. Barbé, R.S.N. Lima, A.F. Costa, L.P. Ribeiro, P.E. Teodoro and L.L. Bhering

Elephant grass is a perennial tropical grass with great potential for energy generation from biomass. The objective of this study was to estimate the genetic diversity among elephant grass accessions based on morpho-agronomic and biomass quality traits and to identify promising genotypes for obtaining hybrids with high energetic biomass production capacity. The expe.. Read More»

Genet. Mol. Res. 16(3): gmr16039803

DOI: 10.4238/gmr16039803

Plant Genetics   Research Article

Diallel crosses for resistance to Macrophomina phaseolina and Thanatephorus cucumeris on cowpea

Authors: L.R.L. Lima, K.J. Damasceno-Silva, M.A. Noronha, D.A. Schurt and M.M. Rocha

This study aimed at identifying and selecting through partial diallel analysis, segregating populations of cowpea resistant to Macrophomina phaseolina and Thanatephorus cucumeris, based on the evaluation of general (GCA) and specific combining ability (SCA), involved in the genetic control of resistance. For this reason, 19 grouped cowpea genotypes, considering the .. Read More»

Genet. Mol. Res. 16(3): gmr16039804

DOI: 10.4238/gmr16039804

Plant Genetics   Research Article

Iron excess in rice: from phenotypic changes to functional genomics of WRKY transcription factors

Authors: V.E. Viana, N. Marini, T. Finatto, I. Ezquer, C. Busanello, R.S. dos Santos, C. Pegoraro, L. Colombo and A. Costa de Oliveira

Iron (Fe) is an essential microelement for all living organisms playing important roles in several metabolic reactions. Rice (Oryza sativa L.) is commonly cultivated in paddy fields, where Fe goes through a reduction reaction from Fe3+ to Fe2+. Since Fe2+ is more soluble, it can reach toxic levels inside plant cells, constituting an important target for studies. Her.. Read More»

Genet. Mol. Res. 16(3): gmr16039694

DOI: 10.4238/gmr16039694

Plant Genetics   Research Article

Minimum number of measurements for evaluating Bertholletia excelsa

Authors: A.B. Baldoni, H. Tonini, F.D. Tardin, S.C.C. Botelho and P.E. Teodoro

Repeatability studies on fruit species are of great importance to identify the minimum number of measurements necessary to accurately select superior genotypes. This study aimed to identify the most efficient method to estimate the repeatability coefficient (r) and predict the minimum number of measurements needed for a more accurate evaluation of Brazil nut tree (B.. Read More»

Genet. Mol. Res. 16(3): gmr16039783

DOI: 10.4238/gmr16039783

Plant Genetics   Research Article

Evolution of the genus Mimivirus based on translation protein homology and its implication in the tree of life

Authors: V.M. Marcelino, M.V.P.C. Espinola, V. Serrano-Solis and S.T. Farias

The natural history of mimiviruses (i.e., viruses that are members of the Mimivirus genus) is a challenge for modern biology. A new domain of life to include these organisms has been proposed from analysis of gene conservation. We analyzed the evolutionary relationship of proteins involved in the translation system, and our data show that mimiviruses are a sister gr.. Read More»

Genet. Mol. Res. 16(3): gmr16039784

DOI: 10.4238/gmr16039784

Human Genetics   Research Article

Mutational screening in the LDLR gene among patients presenting familial hypercholesterolemia in the Southeast of Brazil

Authors: G.A. Molfetta, D.L. Zanette, J.E. Santos and W.A. Silva Jr

Familial hypercholesterolemia (FH) is a dominant, autosomal disease characterized by high LDL levels in blood plasma, and is caused by a defect in the gene encoding the LDL receptor (LDLR). The clinical diagnosis is based on personal and familial history, physical examination findings, and measures of high LDL cholesterol concentrations. LDLR is a cell-surface glyco.. Read More»

Genet. Mol. Res. 16(3): gmr16039226

DOI: 10.4238/gmr16039226

Plant Genetics   Research Article

Analysis of genetic variability of commercial melon cultivars using SSR molecular markers

Authors: N. Carvalho, F.M. Canela, P.H.S. Leite, M.A. Ferreira, V.R. Oliveira, M.F. Santos, N.O.S. Souza and G.S.C. Buso

Taking into account the scenario of melon production (Cucumis melo L.) in Brazil, it is notable the importance of the culture in the fruit market. The preference of the national market is concentrated in the fruits of the yellow type. Therefore, it is possible that the genetic base of the commercial cultivars is relatively narrow due to the loss of important genes i.. Read More»

Genet. Mol. Res. 16(3): gmr16039739

DOI: 10.4238/gmr16039739

Plant Genetics   Research Article

Diallel and prediction (REML/BLUP) for yield components in intervarietal maize hybrids

Authors: I.R. Carvalho, A.J. de Pelegrin, V.J. Szareski, M. Ferrari, T.C. da Rosa, T.S. Martins, N.L. dos Santos, M. Nardino, V.Q. de Souza, A.C. de Oliveira and L.C. da Maia

Genetic improvement is essential to achieve increments in maize (Zea mays L.) grain yield components. It may be obtained through crosses, which enable to exploit the effects of intervarietal heterosis, allelic complementarity, as well as gene actions and effects. This study estimated the components of variance and genetic parameters (REML/BLUP) of an intervarietal d.. Read More»

Genet. Mol. Res. 16(3): gmr16039734

DOI: 10.4238/gmr16039734

Plant Genetics   Research Article

ITS2 as a molecular marker for the identification of Diatraea saccharalis and D. flavipennella and possible infection with Cotesia spp

Authors: M.S. Regueira Neto, R.P. Barros, M.A.J. Morais, V.Q. Balbino and V. Loreto

In Brazil, the species Diatraea flavipennella and D. saccharalis play an important role in the sugar and alcohol agribusiness by causing many damages in sugarcane fields. The egg, larva, pupa, and adult stages are very morphologically similar between these species, and the identification can be confused. The internal transcribed spacer 2 (ITS 2) from ribosomal DNA has important features as evol.. Read More»

Genet. Mol. Res. 16(3): gmr16039732

DOI: 10.4238/gmr16039732

Microbial Genetics   Research Article

A Xanthomonas citri subsp citri hypothetical protein related to virulence contains a non-functional HD domain and is implicated in flagellar motility

Authors: F.C.F. Vieira, A.M. Gonçalves, E.F.R. Mendoza, R.M. Ferreira, M.L.M. Costa, T.S. Balbuena, H.G. Sebinelli, P. Ciancaglini, J.M. Pizauro Junior and J.A. Ferro

Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), severely affects most economically important citrus varieties worldwide. A previous study showed that disruption of the ORF XAC1201 from the Xac 306 strain by transposon Tn5 decreased bacterium virulence in the Rangpur lime host (Citrus limonia L. Osbeck). However, little is kn.. Read More»

Genet. Mol. Res. 16(3): gmr16039728

DOI: 10.4238/gmr16039728

Plant Genetics   Research Article

Characterization of a pepper collection (Capsicum frutescens L.) from Brazil

Authors: M.F. Lima, S.I.C. Carvalho, C.F. Ragassi, L.B. Bianchetti, F.G. Faleiro and F.J.B. Reifschneider

Germplasm banks are essential as sources of genetic variability for plant breeding programs. To characterize a Brazilian Capsicum frutescens collection, 21 malagueta and 5 Tabasco hot pepper accessions were evaluated under field and greenhouse conditions regarding morphological and molecular traits, as well as resistance to viruses. Morphological characterization wa.. Read More»

Genet. Mol. Res. 16(3): gmr16039704

DOI: 10.4238/gmr16039704

Microbial Genetics   Research Article

Characterization and phylogenetic affiliation of Actinobacteria from tropical soils with potential uses for agro-industrial processes

Authors: J.C.M. Dornelas, J.E.F. Figueiredo, C.S. de Abreu, U.G.P. Lana, C.A. Oliveira and I.E. Marriel

Secondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphol.. Read More»

Genet. Mol. Res. 16(3): gmr16039703

DOI: 10.4238/gmr16039703

Plant Genetics   Research Article

Molecular mapping and candidate gene analysis for resistance to powdery mildew in Cucumis sativus stem

Authors: P.N. Liu, H. Miao, H.W. Lu, J.Y. Cui, G.L. Tian, T.C. Wehner, X.F. Gu and S.P. Zhang

Powdery mildew (PM) of cucumber (Cucumis sativus), caused by Podosphaera xanthii, is a major foliar disease worldwide and resistance is one of the main objectives in cucumber breeding programs. The resistance to PM in cucumber stem is important to the resistance for the whole plant. In this study, genetic analysis and gene mapping were implemented with cucumber inbr.. Read More»

Genet. Mol. Res. 16(3): gmr16039680

DOI: 10.4238/gmr16039680

Microbial Genetics   Research Article

Population genetic structure of three species in the genus Astrocaryum G. Mey. (Arecaceae)

Authors: N.P. Oliveira, M.S.P. Oliveira, L.C. Davide and S. Kalisz

We assessed the level and distribution of genetic diversity in three species of the economically important palm genus Astrocaryum located in Pará State, in northern Brazil. Samples were collected in three municipalities for Astrocaryum aculeatum: Belterra, Santarém, and Terra Santa; and in two municipalities for both A. murumuru: Belém and Santo.. Read More»

Genet. Mol. Res. 16(3): gmr16039676

DOI: 10.4238/gmr16039676

Plant Genetics   Research Article

Genetic diversity in a Poincianella pyramidalis (Tul.) L.P. Queiroz population assessed by RAPD molecular markers

Authors: K.S. Belarmino, M.M. Rêgo, R.L.A. Bruno, G.D.A. Medeiros,A.P. Andrade and E.R. Rêgo

Poincianella pyramidalis (Tul.) L.P. Queiroz is an endemic Caatinga (Brazilian savannah biome) species that has been exploited for different purposes, although information is necessary about still existing natural populations. The objective of this study was to evaluate the genetic diversity among 20 P. pyramidalis individuals occurring in a population localized in .. Read More»

Genet. Mol. Res. 16(3): gmr16039663

DOI: 10.4238/gmr16039663

Human Genetics   Research Article

Suppression of the biological activity of neuroglioma cells by down-regulation of miR-1

Authors: Y.Q. Wang, Y. Cai and X.M. Zhong

Neuroglioma is associated with high rates of malignancy, metastasis, and recurrence. Recently, research on the roles of microRNAs (miR) in cancer prognosis has formed an important area of research as differential expression of miRNAs has been observed in different cancers. However, the detailed mechanism by which miRNAs regulate glioma remains unknown. Thus, we inve.. Read More»

Genet. Mol. Res. 16(3): gmr16039256

DOI: 10.4238/gmr16039256

Human Genetics   Research Article

Mac-1 deficiency induces respiratory failure by affecting type I alveolar epithelial cells

Authors: J. Wang, Y.B. Ci, C.L. Liu and H.M. Sun

As a β2 integrin family member, Mac-1 plays an important role in the inflammatory response. Inflammation and lung injury are closely associated, but the involvement of Mac-1 in the occurrence and development of such pathologies remains poorly understood. We aimed to investigate the relationship between Mac-1 deficiency and respiratory failure in Mac-1 knockout .. Read More»

Genet. Mol. Res. 16(3): gmr16039045

DOI: 10.4238/gmr16039045

Plant Genetics   Research Article

Cloning and functional characterization of the HbSYR1 gene encoding a syntaxin-related protein in Tibetan hulless barley (Hordeum vulgare L. var. nudum HK. f.)

Authors: Q.J. Xu, Y.L. Wang, Z.X. Wei, H.J. Yuan, X.Q. Zeng and N. Tashi

Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, “Himalaya 10”; its expression was evaluated during drought stress and rehydrati.. Read More»

Genet. Mol. Res. 16(3): gmr16038909

DOI: 10.4238/gmr16038909

Human Genetics   Research Article

Role of IL17A rs2275913 and rs3748067 polymorphisms in the risk cervical cancer

Authors: A.Q. Niu, Y.H. Cao, H. Wang, X. Zhang, B. Zhu and Z.H. Li

Cervical cancer is a serious public health problem and is associated with high cancer-related mortality in females worldwide. The expression of IL17A can increase the migration and invasiveness of cervical cancer cells by activating the NF-κB signal pathway. Single-nucleotide polymorphisms (SNPs) can alter gene function and protein expression. We examined the .. Read More»

Genet. Mol. Res. 16(3): gmr16038826

DOI: 10.4238/gmr16038826

Bioinformatics   Research Article

Characterization of glucose-tolerant β-glucosidases used in biofuel production under the bioinformatics perspective: a systematic review

Authors: D.C.B. Mariano, C. Leite, L.H.S. Santos, L.F. Marins, K.S. Machado, A.V. Werhli, L.H.F. Lima and R.C. de Melo-Minardi

β-glucosidases are enzymes that catalyze the hydrolysis of oligosaccharides and disaccharides, such as cellobiose. These enzymes play a key role in cellulose degrading, such as alleviating product inhibition of cellulases. Consequently, they have been considered essential for the biofuel industry. However, the majority of the characterized β-glucosidases i.. Read More»

Genet. Mol. Res. 16(3): gmr16039740

DOI: 10.4238/gmr16039740

Human Genetics   Research Article

Clinical features of Mexican patients with Mucopolysaccharidosis type I

Authors: A. Alonzo-Rojo, J.E. García-Ortiz, M. Ortiz-Aranda, M.P. Gallegos-Arreola and L.E. Figuera-Villanueva

Mucopolysaccharidosis type I (MPS-I) is an autosomal recessive lysosomal storage disorder caused by a deficiency or absence of α-l-iduronidase, which is involved in the catabolism of glycosaminoglycans (GAGs). This deficiency leads to the accumulation of GAGs in several organs. Given the wide spectrum of the disease, MPS-I has historically been classified into.. Read More»

Genet. Mol. Res. 16(3): gmr16032602

DOI: 10.4238/gmr16032602

Microbial Genetics   Research Article

A RAPD-PCR-based genetic diversity analysis of Helicoverpa armigera and H. zea populations in Brazil

Authors: H.M. Lopes, C.S. Bastos, L.S. Boiteux, J. Foresti and F.A. Suinaga

Helicoverpa armigera is the most significant pest of agriculture in Asia, Europe, Africa, and Australasia, causing damage to crops greater than US$2 billion annually and until 2013 it was not detected in Brazil. Helicoverpa zea is restricted to the American continent and is important to corn and a secondary pest of cotton and tomatoes. The wide range of crops exploi.. Read More»

Genet. Mol. Res. 16(3): gmr16038757

DOI: 10.4238/gmr16038757

Plant Genetics   Research Article

Genetic effects of in vitro germination and plantlet development in chilli pepper

Authors: P.A. Barroso, M.M. Rêgo, E.R. Rêgo and K.T.C. Ferreira

The objective of this study was to estimate the genetic effects on in vitro germination and development of chili peppers. For this, four genotypes (UFPB-132, -134, -137, and -390) were used as parents. They were crossed in a complete diallel scheme. The parents (4) and hybrid (12) seeds were germinated in glass bottles containing MS media. The following variables we.. Read More»

Genet. Mol. Res. 16(3): gmr16038869

DOI: 10.4238/gmr16038869

Animal Genetics   Research Article

A comparative study on genetic characteristics of two new varieties of Pelodiscus sinensis and their hybrid

Authors: H.Q. Zhang, X.J. Xu, Z.Y. He, J.Z. Shao, X.H. Zhang, Q.H. Meng and F.Y. Huang

Chinese soft-shelled turtle Pelodiscus sinensis has been an important aquaculture species in Southeast Asian countries. To breed a new variety of soft-shelled turtle with excellent properties and to evaluate the effect of hybridization of two turtle strains with a highly different trait phenotype, inheritance, microsatellite loci, and transcriptome analysis were stu.. Read More»

Genet. Mol. Res. 16(3): gmr16039148

DOI: 10.4238/gmr16039148

Plant Genetics   Research Article

A proteomic approach to guarana seed and pericarp maturation

Authors: A.L. Souza, J.L. Lopez-Losano, P.C.S. Angelo, J.N. Souza-Neto, I.B. Cordeiro, S. Astolfi-Filho and E.V. Andrade

Paullinia cupana Kunth var. sorbilis (Mart.) Ducke, the cultivated guarana plant, is native to the Amazon and has been valued for its medicinal, stimulant and energetic properties for centuries. The seeds are the main commercial product of the plant and the source of high amounts of purine alkaloids (caffeine and theobromine) and polyphenols (flavonoids, catechins, .. Read More»

Genet. Mol. Res. 16(3): gmr16039642

DOI: 10.4238/gmr16039642

Human Genetics   Research Article

Atherosclerosis: analysis of the eNOS (T786C) gene polymorphism

Authors: A.M. Barbosa, K.S.F. Silva, M.H. Lagares, D.A. Rodrigues,I.R. da Costa, M.P. Morais, J.V.M. Martins, R.S. Mascarenhas, F.L. Campedelli and K.K.V.O. Moura

The coronary arteriosclerotic disease is the most common cardiovascular disease. Atherosclerosis affects large- and medium-sized arteries leading to severe thrombosis or artery stenosis that could evolve to myocardial infarction, ischemic stroke, ischemic injury of kidneys and intestines, and several other life-threatening clinical manifestations. Nitric oxide has b.. Read More»

Genet. Mol. Res. 16(3): gmr16039708

DOI: 10.4238/gmr16039708

Animal Genetics   Research Article

Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil

Authors: S.P. Silva, L.N.P.P. Silva, E.D.L. Rodrigues, J.F. Cardoso, F.N. Tavares, W.M. Souza, C.M.P. Santos, F.M.S. Martins, I.S. Jesus, T.C. Brito, T.P.C. Moura, M.R.T. Nunes, L.M.N. Casseb, E. Silva Filho and A.R. Casseb

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, .. Read More»

Genet. Mol. Res. 16(3): gmr16039719

DOI: 10.4238/gmr16039719

Plant Genetics   Research Article

Diallel analysis for technological traits in upland cotton

Authors: D.R. Queiroz, F.J.C. Farias, J.J.V. Cavalcanti, L.P. Carvalho, D.G. Neder, L.S.S. Souza, F.C. Farias and P.E. Teodoro

Final cotton quality is of great importance, and it depends on intrinsic and extrinsic fiber characteristics. The objective of this study was to estimate general (GCA) and specific (SCA) combining abilities for technological fiber traits among six upland cotton genotypes and their fifteen hybrid combinations, as well as to determine the effective genetic effects in .. Read More»

Genet. Mol. Res. 16(3): gmr16039727

DOI: 10.4238/gmr16039727

Plant Genetics   Research Article

Morphological, cytogenetic, and molecular characterization of Arachis kuhlmannii Krapov. & W.C. Greg. (Leguminosae)

Authors: A.P. Fávero, A.P.S. Peñaloza, C.R. Lopes and J.F.M. Valls

Arachis kuhlmannii occurs in Mato Grosso and Mato Grosso do Sul States, Brazil. Its area of occurrence partially overlaps with that of other species in the Arachis section. Because of their morphological similarities, these species are often mistaken one for another. This study aimed the correct classification of available accessions as Arachis kuhlmannii, or other .. Read More»

Genet. Mol. Res. 16(3): gmr16039733

DOI: 10.4238/gmr16039733

Animal Genetics   Research Article

Inexpensive metagenomic DNA extraction protocol with high quality from marine sediments contaminated by petroleum hydrocarbons

Authors: I. García-Bautista, T. Toledano-Thompson, E. Dantán-González, J. González-Montilla and R. Valdez-Ojeda

Marine environments are a reservoir of relevant information on dangerous contaminants such as hydrocarbons, as well as microbial communities with probable degradation skills. However, to access microbial diversity, it is necessary to obtain high-quality DNA. An inexpensive, reliable, and effective metagenomic DNA (mgDNA) extraction protocol from marine sediments con.. Read More»

Genet. Mol. Res. 16(3): gmr16039743

DOI: 10.4238/gmr16039743

Plant Genetics   Research Article

High molecular weight glutenin subunits and the classification used in Brazilian wheat industry

Authors: P.I. Chapla, E.S.N. Vieira, F.A. Franco, G.A. Linde, G.J. Silva, N.B. Colauto, V.S. Marchioro and I. Schuster

Industrial wheat quality flour is related to gluten amount in grain. This study aimed to evaluate the relationship between high molecular weight (HMW) glutenins obtained by SDS-PAGE and gluten strength (W) obtained by the alveograph test and cropping environmental effect on wheat flour quality for Brazilian industry. Fifty-one cultivars/breeding lines were evaluated.. Read More»

Genet. Mol. Res. 16(3): gmr16039744

DOI: 10.4238/gmr16039744

Plant Genetics   Research Article

Genetic diversity and population structure analysis in Perilla frutescens from Northern areas of China based on simple sequence repeats

Authors: S.J. Ma, K.J. Sa, T.K. Hong and J.K. Lee

In this study, 21 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity and population structure among 77 Perilla accessions from high-latitude and middle-latitude areas of China. Ninety-five alleles were identified with an average of 4.52 alleles per locus. The average polymorphic information content (PIC) and genetic diversity values wer.. Read More»

Genet. Mol. Res. 16(3): gmr16039746

DOI: 10.4238/gmr16039746

Animal Genetics   Research Article

Cadmium accumulation and metallothionein gene expression in the liver of swamp eel (Monopterus albus) collected from the Mae Sot District, Tak Province, Thailand

Authors: M. Wahid, S. Prasarnpun and N. Yimtragool

Cadmium (Cd) is produced mainly as a by-product of zinc mining. In Thailand, the largest zinc mine is located in the Mae Sot district, Tak Province. Samples of Monopterus albus were collected from paddy fields in 4 sites, three downstream and one upstream from the zinc mine. The upstream site was considered to be uncontaminated while the three downstream sites were .. Read More»

Genet. Mol. Res. 16(3): gmr16039748

DOI: 10.4238/gmr16039748

Plant Genetics   Research Article

Genetic parameters and selection of soybean lines based on selection indexes

Authors: F.G. Teixeira, O.T. Hamawaki, A.P.O. Nogueira, R.L. Hamawaki, G.L. Jorge, C.L. Hamawaki, B.Q.V. Machado and A.J.O. Santana

Defining selection criteria is important to obtain promising genotypes in a breeding program. The objective of this study was to estimate genetic parameters for agronomic traits and to perform soybean line selection using selection indices. The experiment was conducted at an experimental area located at Capim Branco farm, belonging to the Federal University of Uberl.. Read More»

Genet. Mol. Res. 16(3): gmr16039750

DOI: 10.4238/gmr16039750

Plant Genetics   Research Article

Estimates of repeatability coefficients and the number of the optimum measure to select superior genotypes in Annona muricata L.

Authors: C.F.B. Sanchéz, R.S. Alves, A.d.P. Garcia, P.E. Teodoro, L.A. Peixoto, L.A. Silva, L.L. Bhering and M.D.V. Resende

The aim of this study was to evaluate repeated measures over the years to estimate repeatability coefficient and the number of the optimum measure to select superior genotypes in Annona muricata L. The fruit production was evaluated over 16 years in 71 genotypes without an experimental design. The estimation of variance components and the prediction of the permanent.. Read More»

Genet. Mol. Res. 16(3): gmr16039753

DOI: 10.4238/gmr16039753

Plant Genetics   Research Article

Chromosome variations and diversity of Epidendrum ibaguense Lindl. (Orchidaceae) on the Tepequém’s Tepuy, Roraima, Brazil

Authors: S.R. Nóbrega, A.L.F. Coelho, C.F. Verola, I.R. Costa, R. Vilaça, F.J.F. Luz and W.F. Araújo

Studies addressing chromosome variations have elucidated many points regarding the taxonomy of the Orchidaceae. Epidendrum L. besides being one the largest orchid genera, present remarkable morphological, and inter- and intraspecific chromosome variations. Thus, based on a previous report on flower color variation in individuals of E. ibaguense (magenta, pink, white.. Read More»

Genet. Mol. Res. 16(3): gmr16039754

DOI: 10.4238/gmr16039754

Human Genetics   Research Article

Interleukin-18 (rs187238) and glucose transporter 4 (rs5435) polymorphisms in Euro-Brazilians with type 1 diabetes

Authors: Y. Al-Lahham, A.K.B. Mendes, E.M. Souza, D. Alberton, F.G.M. Rego, G. Valdameri and G. Picheth

Type 1 diabetes (T1D) is an autoimmune disease with a strong genetic component that has been associated with several genetic loci. Interleukin 18 (IL-18) is a potent proinflammatory cytokine, which is involved in the innate and adaptive immune responses, and in the pathogenesis of various diseases including T1D. Glucose transporter 4 (GLUT4) is known to be an insuli.. Read More»

Genet. Mol. Res. 16(3): gmr16039755

DOI: 10.4238/gmr16039755

Plant Genetics   Research Article

Contemporary pollen and seed dispersal in natural populations of Bertholletia excelsa (Bonpl.)

Authors: A.B. Baldoni, L.H.O. Wadt, T. Campos, V.S. Silva, V.C.R. Azevedo, L.R. Mata, A.A. Botin, N.O. Mendes, F.D. Tardin, H. Tonini, E.S.S. Hoogerheide and A.M. Sebbenn

Due to the nutritional content and commercial value of its seeds, Bertholletia excelsa is one of the most important species exploited in the Amazon region. The species is hermaphroditic, insect pollinated, and its seeds are dispersed by barochory and animals. Because the fruit set is dependent on natural pollinator activity, gene flow plays a key role in fruit produ.. Read More»

Genet. Mol. Res. 16(3): gmr16039756

DOI: 10.4238/gmr16039756

Microbial Genetics   Research Article

Novel multiplex PCR for detection of diarrheagenic Escherichia coli strains isolated from stool and water samples

Authors: J.W. Vendruscolo, T.L. Waldrich, G.I.A. Saikawa, J.S. Pelayo, R.K.T. Kobayashi, G. Nakazato, S.P.D. Rocha

Diarrhea is considered the second most common cause of infant mortality worldwide. The disease can be caused by many different pathogens, including diarrheagenic Escherichia coli (DEC), which includes the pathotypes enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and enteropathoge.. Read More»

Genet. Mol. Res. 16(3): gmr16039760

DOI: 10.4238/gmr16039760

Human Genetics   Research Article

Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential

Authors: D.C. Silva, L.E.C. Diniz, A.F. Blank, D.A.C. Nizio, J.A.O. Pinto, K.L.G. Pereira and M.F. Arrigoni-Blank

Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore, ex Hyptis fruticosa Salzm. ex Benth. is an aromatic and medicinal plant of the family Lamiaceae, found mainly in regions with intense anthropic activity. Information on the genetic diversity of this species is scarce. However, it can be assessed using molecular markers that identify the level of d.. Read More»

Genet. Mol. Res. 16(3): gmr16039749

DOI: 10.4238/gmr16039749

Human Genetics   Research Article

Synergic effect of oral contraceptives, GSTP1 polymorphisms, and high-risk HPV infection in development of cervical lesions

Authors: B.S. Chagas, A.P.A.D. Gurgel, S.S.L. Paiva Júnior, R.C.P. Lima,M.N. Cordeiro, R.R. Moura, A.V.C. Coelho, K.C.G. Nascimento,J.C. Silva Neto, S. Crovella and A.C. Freitas

Human papillomavirus (HPV) infection is considered a risk factor for cervical cancer. Even if the high-risk HPV (HR-HPV) infection is necessary, environmental co-factors and genetic susceptibility also play an important role in cervical cancer development. In this study, a possible association of rs1695 GSTP1 polymorphisms, HR-HPV infection, and oral contraceptive u.. Read More»

Genet. Mol. Res. 16(3): gmr16039742

DOI: 10.4238/gmr16039742

Plant Genetics   Research Article

Stability of annual biomass energy production of elephant grass (Pennisetum purpureum Schum.) genotypes in the Northern region of the Rio de Janeiro State, Brazil

Authors: M.S.B. Araújo, R.F. Daher, B.R.S. Menezes, G.A. Gravina, V.B. Silva, A.T. Amaral Júnior, E.V. Rodrigues, B.O. Almeida and N.J. Ponciano

The objectives of this study were to estimate the stability of dry biomass production of elephant grass genotypes under an annual harvest regime, in soil-climatic conditions of the Northern region of the Rio de Janeiro State, Brazil, and to compare methodologies for stability analyses of Yates and Cochran (YC), Plaisted and Peterson (PP), Annicchiarico (ANN), Lin an.. Read More»

Genet. Mol. Res. 16(3): gmr16039041

DOI: 10.4238/gmr16039041

Plant Genetics   Research Article

Genetic diversity analysis in Malaysian giant prawns using expressed sequence tag microsatellite markers for stock improvement program

Authors: K.H. Atin, A. Christianus, N. Fatin, A.C. Lutas, M. Shabanimofrad and B. Subha

The Malaysian giant prawn is among the most commonly cultured species of the genus Macrobrachium. Stocks of giant prawns from four rivers in Peninsular Malaysia have been used for aquaculture over the past 25 years, which has led to repeated harvesting, restocking, and transplantation between rivers. Consequently, a stock improvement program is now important to avoi.. Read More»

Genet. Mol. Res. 16(3): gmr16035685

DOI: 10.4238/gmr16035685

Human Genetics   Research Article

Correlation analysis of ADAMTS-4, VCAM-1, and TAK1 expression in cartilage tissue from spine tuberculosis

Authors: Z.X. Qiu, Z.S. Sha, X.M. Che and M.Y. Wang

A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) can effectively degrade articular cartilage matrix proteoglycan and damage the intervertebral disc of spinal tuberculosis patients, resulting in deterioration of the physical properties of articular cartilage. Transforming growth factor β activated kinase 1 (TAK1) is similar to vascular.. Read More»

Genet. Mol. Res. 16(3): gmr16038961

DOI: 10.4238/gmr16038961

Human Genetics   Research Article

miR-367 promotes uveal melanoma cell proliferation and migration by regulating PTEN

Authors: J.W. Ling, P.R. Lu, Y.B. Zhang, S. Jiang and Z.C. Zhang

We aimed to investigate the biological role of miR-367 in uveal melanoma cell growth and migration, and the underlying mechanism responsible. Quantitative real-time polymerase chain reaction was performed to evaluate miR-367 expression in uveal melanoma tissue samples and cell lines. A miR-367 mimic, miR-367 inhibitor, and negative control oligonucleotide were trans.. Read More»

Genet. Mol. Res. 16(3): gmr16039067

DOI: 10.4238/gmr16039067

Plant Genetics   Research Article

Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials

Authors: R.M.O. Pires, M.A.B. Àvila, D.G. Leite, H.O. Santos, G.A. Souza and E.V.R. Von Pinho

With the imminence of global climate changes that affect the temperature and the rainfall uniformity, it is growing the concern about the adaptation of crops to the water deficit. Thus, the objective of this study was to evaluate alterations in physiological and enzymatic mechanisms during the germination process of sesame seeds under different water availability. T.. Read More»

Genet. Mol. Res. 16(3): gmr16039425

DOI: 10.4238/gmr16039425

Plant Genetics   Research Article

Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers

Authors: R.B. Feitosa-Alcantara, A.V.C. Silva, A.F. Blank, C.S. Almeida, S.V. Alvares-Carvalho and M.F. Arrigoni-Blank

Hyptis pectinata, popularly known as ‘sambacaitá’ or ‘canudinho’, is a medicinal and aromatic species widely used in the Brazilian Northeast. In Sergipe, the excessive extraction of natural resources may reduce the genetic variability of native plants. Thus, molecular markers have frequently been applied to the characterization of gene.. Read More»

Genet. Mol. Res. 16(3): gmr16039603

DOI: 10.4238/gmr16039603

Human Genetics   Research Article

First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells

Authors: E.S. Marques, M.S.F. Tsuboy, J.C.T. Carvalho, P.C.P. Rosa, F.F. Perazzo, I.O.M. Gaivão and E.L. Maistro

Euterpe oleracea Mart., popularly known as “açaí”, is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. .. Read More»

Genet. Mol. Res. 16(3): gmr16039700

DOI: 10.4238/gmr16039700

Plant Genetics   Research Article

First record of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in Paraguay confirmed by molecular evidence

Authors: J.V.C. Guedes, J.A. Arnemann, L.E. Curioletti, L.M. Burtet, M.L. Ramírez-Paredes, D. Noschang, F. Irala de Oliveira and W.T. Tay

We provided the first scientific record of Melanagromyza sojae (Zehntner, 1900), through molecular characterization of partial mtDNA COI gene, that confirms the occurrence of this pest in Paraguay. Previously reported in Brazil, an outbreak of larvae of M. sojae known as the soybean stem fly (SSF) that belongs to the family Agromyzidae, was also noted in soybean fie.. Read More»

Genet. Mol. Res. 16(3): gmr16039707

DOI: 10.4238/gmr16039707

Animal Genetics   Research Article

Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus)

Authors: F.F. Chen, C.Y. Ma, L.P. Yan, H. Zhang, W. Wang, Y. Zhang and L.B. Ma

In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information con.. Read More»

Genet. Mol. Res. 16(3): gmr16039712

DOI: 10.4238/gmr16039712

Plant Genetics   Research Article

Analysis of Mendelian inheritance and genetic linkage in microsatellite loci of Eucalyptus urophylla S.T. Blake

Authors: S. Pupin, L.N. Rosse, I.C.G. Souza, J. Cambuim, C.L. Marino, M.L.T. Moraes and A.M. Sebbenn

Eucalyptus urophylla is an important species in the Brazilian forest sector due to its rapid growth rates and resistance to disease. The aim of this study was to verify Mendelian inheritance, genetic linkage, and genotypic disequilibrium for 15 microsatellite loci, with the goal of producing a robust set of genetic markers. Mendelian inheritance and genetic linkage .. Read More»

Genet. Mol. Res. 16(3): gmr16039713

DOI: 10.4238/gmr16039713

Plant Genetics   Research Article

Antioxidant activity and chemical composition of oleoresin from leaves and flowers of Brunfelsia uniflora

Authors: L.F. Jorge, A.B. Meniqueti, R.F. Silva, K.A. Santos, E.A. Da Silva, J.E. Gonçalves, C.M. De Rezende, N.B. Colauto, Z.C. Gazim and G.A. Linde

In this study, the temperature and pressure of supercritical CO2 extraction were evaluated to obtain oleoresin of Brunfelsia uniflora leaves and flowers. The oleoresin compounds were identified by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by three different methods. The highest oleoresin yields were 3.32% at 40°C and 200 bar fo.. Read More»

Genet. Mol. Res. 16(3): gmr16039714

DOI: 10.4238/gmr16039714

Plant Genetics   Research Article

Correlation study of resistance components in the selection of Capsicum genotypes resistant to the fungus Colletotrichum gloeosporioides

Authors: A.C. Maracahipes, J.W.S. Correa, P.E. Teodoro, K.L. Araújo, M.A.A. Barelli and L.G. Neves

Anthracnose is among the major diseases of the Capsicum culture. It is caused by different species of the genus Colletotrichum, which may result in major damages to the cultivation of this genus. Studies aiming to search for cultivars resistant to diseases are essential to reduce financial and agricultural losses. The objective of this study was to evaluate the corr.. Read More»

Genet. Mol. Res. 16(3): gmr16039720

DOI: 10.4238/gmr16039720

Human Genetics   Research Article

Analysis of p53 gene polymorphism (codon 72) in symptomatic patients with atherosclerosis

Authors: M.H. Lagares, K.S.F. Silva, A.M. Barbosa, D.A. Rodrigues, I.R. Costa, J.V.M. Martins, M.P. Morais, F.L. Campedelli and K.K.V.O. Moura

Atherosclerosis is a multifactorial pathological disease that alters the morphology and function of arterial walls. The atheroma growth leads to vessel hardening and lumen narrowing, limiting the blood flow. The atheroma plaque can eventually break, expose highly thrombogenic material and lead to platelet activation and subsequent formation of a thrombus that may bl.. Read More»

Genet. Mol. Res. 16(3): gmr16039721

DOI: 10.4238/gmr16039721

Plant Genetics   Research Article

Evaluation of genotype x environment interactions in cotton using the method proposed by Eberhart and Russell and reaction norm models

Authors: R.S. Alves, P.E. Teodoro, F.C. Farias, F.J.C. Farias, L.P. Carvalho, J.I.S. Rodrigues, L.L. Bhering and M.D.V. Resende

Cotton produces one of the most important textile fibers of the world and has great relevance in the world economy. It is an economically important crop in Brazil, which is the world’s fifth largest producer. However, studies evaluating the genotype x environment (G x E) interactions in cotton are scarce in this country. Therefore, the goal of this study was t.. Read More»

Genet. Mol. Res. 16(3): gmr16039726

DOI: 10.4238/gmr16039726

Plant Genetics   Research Article

Stability and adaptability of soybean cultivars in Minas Gerais

Authors: I.O. Soares, A.T. Bruzi, E.V. Zambiazzi, S.R. Guilherme, M.C. Bianchi, K.B. Silva, V. Fronza and C.M. Teixeira

Genotypes x environment (G x E) interaction consists of different behavior of genotypes cultivated in different environments. This interaction occurs due to the performance variation of each genotype in different environments. To reduce the effect of the interaction in soybean crops, some studies have been reported in the literature to study their adaptability and s.. Read More»

Genet. Mol. Res. 16(3): gmr16039730

DOI: 10.4238/gmr16039730

Plant Genetics   Research Article

Genotypic gain with simultaneous selection of production, nutrition, and culinary traits in cowpea crosses and backcrosses using mixed models

Authors: D.G. Oliveira, M.M. Rocha, K.J. Damasceno-Silva, F.V. Sá, L.R.L. Lima, and M.D.V. Resende

The aim of this study was to estimate the genotypic gain with simultaneous selection of production, nutrition, and culinary traits in cowpea crosses and backcrosses and to compare different selection indexes. Eleven cowpea populations were evaluated in a randomized complete block design with four replications. Fourteen traits were evaluated, and the following parame.. Read More»

Genet. Mol. Res. 16(3): gmr16039736

DOI: 10.4238/gmr16039736

Human Genetics   Research Article

Polymorphisms of interleukin 6 in Down syndrome individuals: a case-control study

Authors: M.F. Mattos, L. Uback, P.M. Biselli-Chicote, J.M. Biselli, E.M. Goloni-Bertollo and E.C. Pavarino

Down syndrome (DS) individuals present impaired adaptive immune system. However, the etiology of the immunological deficiency in these individuals is not completely understood. This study investigated the frequency of interleukin 6 polymorphisms (rs1800795, rs1800796, and rs1800797) in individuals with DS and individuals without the syndrome. The study included 282 .. Read More»

Genet. Mol. Res. 16(3): gmr16039738

DOI: 10.4238/gmr16039738

Microbial Genetics   Abstracts

Supplementation levels of exogenous alpha-amylase in broilers diets

Authors: H.B. Oliveira, M.I.A. Silva and F.R. Mesquita

This study aimed to evaluate the supplementation levels of an exogenous alpha-amylase in broilers diets and compare two indicators in determining the diets energy. The experiment was divided into two parallel evaluations, being one of performance and the other of metabolism. In performance assay, 1,700 one-day-old Cobb-500 male chicks were used. The animals were dis.. Read More»

Genet. Mol. Res. 16(3): gmr16038766

DOI: 10.4238/gmr16038766

Human Genetics   Research Article

MiR-200c inhibits metastasis of breast tumor via the downregulation of Foxf2

Authors: T. Zhang, J.G. Wan, J.B. Liu and M. Deng

The forkhead box F2 (Foxf2) gene suppresses epithelial-mesenchymal transition via the modulation of transcription of zinc finger E-box-binding homeobox 1 (Zeb1) and epithelial (E)-cadherin, thereby inhibiting tumor metastasis. Additionally, the specific binding of microRNA (miR)-200c to Foxf2 mRNA impedes metastatic pulmonary cancer. However, the role of miR-200c in.. Read More»

Genet. Mol. Res. 16(3): gmr16038971

DOI: 10.4238/gmr16038971

Plant Genetics   Research Article

Internal amplification control of PCR for the Glu1-Dx5 allele in wheat

Authors: H.N. Heim, E.S.N. Vieira, L.R.T. Polo, N.K. Lima, G.J. Silva, G.A. Linde, N.B. Colauto and I. Schuster

One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in genetic breeding programs besides the loss o.. Read More»

Genet. Mol. Res. 16(3): gmr16039718

DOI: 10.4238/gmr16039718

Plant Genetics   Research Article

Morphological diversity of cassava accessions of the south-central mesoregion of the State of Mato Grosso, Brazil

Authors: B.W. Zago, M.A.A. Barelli, E.S.S. Hoogerheide, C.L. Corrêa,G.I.S. Delforno and C.J. da Silva

Genetic variability of cassava (Manihot esculenta Crantz) in Brazil is wide, being this the result of natural and cultural selection during pre- and post-domestication of the species in different environments. Given the number of species of the genus found in the region (38 of a total of 98 species), the central region of Brazil was defined as the primary center of .. Read More»

Genet. Mol. Res. 16(3): gmr16039725

DOI: 10.4238/gmr16039725

Animal Genetics   Research Article

Association of CYP2C19*2 with hyperlipidemia in patients with the wild-type CYP2C9

Authors: K. Khalighi, G. Cheng, S. Mirabbasi, B. Khalighi, Y. Wu and W. Fan

Arachidonic acids are metabolized by cytochromes P450 (CYP450) to epoxyeicosatrienoic acids (EETs). EETs have many cardiovascular protective effects such as vasodilation, anti-hypertension, anti-inflammation, lipid-lowering effects, and favorable effects on glucose homeostasis. This study aimed to investigate whether gene polymorphism of cytochrome P450 CYP2C9 and C.. Read More»

Genet. Mol. Res. 16(3): gmr16039737

DOI: 10.4238/gmr16039737

Plant Genetics   Research Article

Univariate, multivariate techniques and mixed models applied to the adaptability and stability of wheat in the Rio Grande do Sul State

Authors: V.J. Szareski, I.R. Carvalho, K. Kehl, A.M. Levien, M. Nardino, G.H. Demari, F. Lautenchleger, V.Q. de Souza, T. Pedó and T.Z. Aumonde

The wheat crop presents sensitivity to the environmental conditions culminating in the genotype x environment interaction, being crucial the use of different methodologies to guide the positioning of genotypes to certain cultivation environments. The objective of this study was to estimate the adaptability and phenotypic stability of wheat genotypes grown in the Sta.. Read More»

Genet. Mol. Res. 16(3): gmr16039735

DOI: 10.4238/gmr16039735

Plant Genetics   Research Article

Genetic dissimilarity of common bean lines for agronomic and biofortification traits

Authors: S.M. Maziero, N.D. Ribeiro and C.R. Casagrande

The evaluation of the genetic dissimilarity of common bean lines obtained by the breeding program is significant to identify duplicate accessions, to select superior lines, and to plan new hybrid combinations. The objectives of this study were to evaluate the genetic dissimilarity of common bean genotypes for agronomic and mineral biofortification traits, select sup.. Read More»

Genet. Mol. Res. 16(3): gmr16039731

DOI: 10.4238/gmr16039731

Plant Genetics   Research Article

Comparison of eight methods to isolate genomic DNA from Hancornia speciosa

Authors: V.M. Almeida, G.A. Luz, P.P. Martins, M.F.C. Gomes, M.F. Costa, P.S.C. Lima and S.E.S. Valente

Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosio.. Read More»

Genet. Mol. Res. 16(3): gmr16039724

DOI: 10.4238/gmr16039724

Human Genetics   Research Article

Pharmacogenetics-based warfarin dose prediction algorithm for patients with heart valve replacement in Southeast China

Authors: M.Q. Lin, J. Zhang, H.F. Qiu, X.B. Xie and H.T. Song

This study was designed to analyze the influence of polymorphisms in CYP2C9, VKORC1, CYP4F2, GGCX, and EPHX1 genes on the optimal warfarin dosage in patients who have undergone artificial heart valve replacement and establish an algorithm for use by physicians in Southeast China to predict each patient’s optimal steady-state warfarin dose. One-hundred and nine.. Read More»

Genet. Mol. Res. 16(3): gmr16038881

DOI: 10.4238/gmr16038881

Plant Genetics   Research Article

Genetic variability in banana diploids and nonparametric statistics of fragments associated with natural fruit finger drop

Authors: M.A. Rodrigues, E.P. Amorim, C.F. Ferreira, C.A.S. Ledo and J.R.F. Santana

Natural fruit finger drop in bananas is one of the major concerns of producers as well as consumers. The aim of this study was to estimate the genetic diversity among 15 diploid bananas with different levels of resistance to finger drop (RFD) using SSR and ISSR (inter-simple sequence repeat) markers, and assess the possibility of association of bands with RFD variab.. Read More»

Genet. Mol. Res. 16(3): gmr16039554

DOI: 10.4238/gmr16039554

Plant Genetics   Research Article

QTLs of morpho-agronomic traits in the Jalo x Small White common bean population

Authors: D.A. Souza, M. Balestre, A.K.A. Pamplona, L.R. Pinheiro, F.C. Alves, D.R. Pereira and J.B. Santos

In this study, we aimed to estimate the relationship between some common bean traits using molecular markers and applying QTL mapping. We used a segregating population derived from a crossing between common bean cultivars, Jalo and Small White, in the Southern State of Minas Gerais. Of F2 plants, 190 F2:3 progenies were generated. Phenotypic measures related to the .. Read More»

Genet. Mol. Res. 16(3): gmr16039686

DOI: 10.4238/gmr16039686

Plant Genetics   Research Article

Farmer variety exchange along Amazonian rivers influences the genetic structure of manioc maintained in a regional Brazilian Gene Bank

Authors: S.B. Sousa, G.F. Silva, M.C. Dias, C.R. Clement and N.R. Sousa

Knowledge of agro-morphological and genetic variability of germplasm diversity is a prerequisite for its use in breeding. The aim of this study was to characterize the genetic diversity of the manioc (Manihot esculenta) Active Germplasm Bank (AGB) at Embrapa Amazônia Ocidental using microsatellite markers. We evaluated 470 accessions collected along five of th.. Read More»

Genet. Mol. Res. 16(3): gmr16039690

DOI: 10.4238/gmr16039690

Human Genetics   Research Article

Association of monocyte chemoattractant protein-1 rs1024611 and telomerase rs2736100 polymorphisms with susceptibility to pulmonary tuberculosis in Han Chinese population

Authors: G.H. Cheng, X.L. Wang, X.T. Yuan, Q. Xin, Q. Sun, K.L. Li, C. Sun, Z.M. Lin, Y. Luan, Y.S. Wang, F. Kong and D.W. Xu

Pulmonary tuberculosis (PTB) is caused by Mycobacterium tuberculosis infection and accumulated evidence reveals the genetic context of its infection phenotypes. Monocyte chemoattractant protein-1 (MCP-1) rs1024611 variant is shown to be associated with PTB susceptibility in some studies, but significant disparities exist. In addition, telomerase plays a key role in .. Read More»

Genet. Mol. Res. 16(3): gmr16039697

DOI: 10.4238/gmr16039697

Plant Genetics   Research Article

Soybean genotype analysis for cyst nematode resistance

Authors: B.C.M. Juliatti, I.F. Beloti, L.C. Pena and F.C. Juliatti

Some species of soybean cultivars have the characteristic of resistance or alteration in survival skills, and perpetuation of nematodes (female index - FI), whose process is activated when the nematode parasitism starts and the development of new resistant cultivars is primordial to the preservation of plant productivity. The objective of this study was to identify .. Read More»

Genet. Mol. Res. 16(3): gmr16039705

DOI: 10.4238/gmr16039705

Plant Genetics   Research Article

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Authors: V.D. Rocha, P.V. Tiago, A.V. Tiago, E.C.M. Pedri, E.S. Cardoso and A.A.B. Rossi

Hymenaea courbaril L. is a tropical tree species with economic and medicinal potential largely used in programs for the recovery of degraded areas. The use of molecular markers in genetic studies can help in the definition of forest restoration strategies. This study was conducted to analyze the genetic diversity among H. courbaril (‘jatobá’) geno.. Read More»

Genet. Mol. Res. 16(3): gmr16039706

DOI: 10.4238/gmr16039706

Microbial Genetics   Research Article

Antimicrobial activity of essential oil and growth of Ocimum basilicum (L.) inoculated with mycorrhiza and humic substances applied to soil

Authors: F. Morelli, L. Ferarrese, C.L. Munhoz and O. Alberton

Mycorrhizal association increases the absorption of water and nutrients by plants, and thus, affects their metabolism. This study aimed at assessing the single and conjoint effects of the arbuscular mycorrhizal fungus (AMF) Claroideoglomus etunicatum and soil’s humic substances (HS) on the composition of essential oil (EO), its antimicrobial activity, and deve.. Read More»

Genet. Mol. Res. 16(3): gmr16039710

DOI: 10.4238/gmr16039710

Plant Genetics   Research Article

Investigation on possible occurrence of somaclonal effects in zygotic and embryonic cotton structures based on agronomical traits

Authors: T.C. Soares, J.M.F.C. Carvalho, L.M. Lima, R.M.M. Freire,C.R.C. Silva, P.A. Melo Filho and R.C. Santos

Zygotic and embryonic cotton structures were used in in vitro procedures to obtain stable plants. Zygotes (24, 48, and 72 h) and immature embryos (10 and 25 days after fertilization) were grown in MS medium supplemented with glucose, indoleacetic acid, kinetin, and activated charcoal. Regenerated seedlings were acclimatized and grown in 10-kg pots for further phenol.. Read More»

Genet. Mol. Res. 16(3): gmr16039711

DOI: 10.4238/gmr16039711

Plant Genetics   Research Article

Efficiency of selection per se and in single-cross hybrids for disease resistance in maize

Authors: R.B. Camargos, R.G. Von Pinho, M. Balestre, R.A.D.C. Ferreira, K.O.G. Dias, L.P.M. Pires and V.F. Souza

Plant breeding strategies are constantly refined to increase efficiency during the selection process. Consequently, the aims of this study were to examine genetic control and efficiency of selection based on the per se performance of maize lines and based on hybrid performance in reaction to white spot, common rust, and ear rot diseases in maize breeding programs. W.. Read More»

Genet. Mol. Res. 16(3): gmr16039716

DOI: 10.4238/gmr16039716

Plant Genetics   Research Article

Diallel analysis for agronomic traits in upland cotton in semi-arid zones in Brazil

Authors: D.R. Queiroz, F.J.C. Farias, J.J.C. Vasconcelos, L.P. Carvalho, D.G. Neder, L.S.S. Souza, F.C. Farias and P.E. Teodoro

This study aimed to estimate the general- (GCA) and specific-combining ability (SCA) and obtain information on the genetic control of agronomic traits among six upland cotton genotypes and their hybrid combinations. In 2015, six cotton genotypes (FM 993, CNPA 04-2080, PSC 355, TAM B 139-17, IAC 26, and TAMCOT-CAMD-E), and fifteen hybrid combinations were evaluated a.. Read More»

Genet. Mol. Res. 16(3): gmr16039677

DOI: 10.4238/gmr16039677

Human Genetics   Research Article

Genetic diversity analyses of tropical goats from some countries of Middle East

Authors: R.M. Al-Atiyat

The genetic structure of 10 goat breeds from four countries of the Middle East and Horn of Africa was investigated at 17 microsatellite loci. The average allele number and richness were 9.65 and 5.78, respectively. The mean expected heterozygosity per population was 0.703, 0.715, 0.719, 0.699, 0.749, 0.719, 0.731, 0.721, 0.719, and 0.730 for Somali from Somalia, Toh.. Read More»

Genet. Mol. Res. 16(3): gmr16039701

DOI: 10.4238/gmr16039701

Plant Genetics   Research Article

Selection of Jatropha full-sib families based on genotypic adaptability and stability via mixed models

Authors: E.V. Rodrigues, P.E. Teodoro, L.A. Peixoto, L.A. Silva, B.G. Laviola and L.L. Bhering

The objectives of this study were to estimate the genetic parameters for Jatropha full-sib families and to select superior genotypes based on grain yield, adaptability, and stability simultaneously to be used for cloning and crossings. Grain yield was evaluated in thirteen full-sib families in a randomized block design for 5 years. The harmonic mean of the relative .. Read More»

Genet. Mol. Res. 16(3): gmr16039722

DOI: 10.4238/gmr16039722

Plant Genetics   Research Article

Diversity and genetic structure of the native Brazil nut tree (Bertholletia excelsa Bonpl.) population

Authors: J.C. Cabral, A.B. Baldoni, H. Tonini, V.C.R. Azevedo, L.D. Giustina, A.V. Tiago and A.A.B. Rossi

The present study was carried out in a native Brazil nut tree population (Bertholletia excelsa Bonpl., Lecythidaceae) to assess its genetic diversity and structure. Ten microsatellite markers were used to genotype 198 adult trees (B. excelsa). The population presented high genetic diversity and inbreeding absence rates. The empirical Bayesian method showed three dis.. Read More»

Genet. Mol. Res. 16(3): gmr16039702

DOI: 10.4238/gmr16039702

Human Genetics   Research Article

Steroid metabolism gene polymorphisms and their implications on breast and ovarian cancer prognosis

Authors: E.V.W. dos Santos, L.N.R. Alves and I.D. Louro

A role for estrogen in the etiology of breast and ovarian cancers has been suggested; therefore, genetic polymorphisms in steroid metabolism genes could be involved in the carcinogenesis of these tumors. We have aimed to investigate the role of GSTP1 and CYP17 polymorphisms and their correlation with MSI (microsatellite instability) and LOH (loss of heterozygosity) .. Read More»

Genet. Mol. Res. 16(3): gmr16039691

DOI: 10.4238/gmr16039691

Plant Genetics   Research Article

Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers

Authors: S.I.C. Carvalho, L.B. Bianchetti, C.F. Ragassi, C.S.C. Ribeiro, F.J.B. Reifschneider, G.S.C. Buso and F.G. Faleiro

Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatel.. Read More»

Genet. Mol. Res. 16(3): gmr16039689

DOI: 10.4238/gmr16039689

Plant Genetics   Research Article

Environmental stratifications for soybean cultivar recommendation and its consistency over time

Authors: R.M. Pacheco, J.B. Duarte, R.G. Branquinho and P.I.D.M. Souza

Stratification of environments is a strategy to capitalize genotype x environment (GxE) interaction, which can optimize the process of assessment and cultivar recommendation, increasing productivity in a target environmental population. The objective of this study was to assess environmental stratification methods based on the analysis of GxE interaction, to identif.. Read More»

Genet. Mol. Res. 16(3): gmr16039693

DOI: 10.4238/gmr16039693

Human Genetics   Research Article

Association of endothelin-1 gene polymorphisms with essential hypertension in a Chinese population

Authors: Z. Fang, M. Li, Z. Ma and G. Tu

Endothelin-1 (ET-1) is the most potent endogenous vasoconstrictor and is involved in several vascular disorders such as hypertension. Its strong interaction with other vasoactive hormone systems suggests that the ET-1 gene (EDN1) is a potential candidate molecule that influences the risk of developing hypertension. Recently, two single nucleotide polymorphisms in ED.. Read More»

Genet. Mol. Res. 16(3): gmr16037446

DOI: 10.4238/gmr16037446

Plant Genetics   Research Article

An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae)

Authors: S.R. Arruda, D.G. Pereira, M.M. Silva-Castro, M.G. Brito and A.M. Waldschmidt

Some species are characterized by a high content of tannins, alkaloids, and phenols in their leaves. These secondary metabolites are released during DNA extraction and might hinder molecular studies based on PCR (polymerase chain reaction). To provide an efficient method to extract DNA, Mimosa tenuiflora, an important leguminous plant from Brazilian semiarid region .. Read More»

Genet. Mol. Res. 16(3): gmr16039063

DOI: 10.4238/gmr16039063

Plant Genetics   Research Article

Variability among Capsicum baccatum accessions from Goiás, Brazil, assessed by morphological traits and molecular markers

Authors: A.L.A. Martinez, J.S.P. Araújo, C.F. Ragassi, G.S.C. Buso and F.J.B. Reifschneider

Capsicum peppers are native to the Americas, with Brazil being a significant diversity center. Capsicum baccatum accessions at Instituto Federal (IF) Goiano represent a portion of the species genetic resources from central Brazil. We aimed to characterize a C. baccatum working collection comprising 27 accessions and 3 commercial cultivars using morphological traits .. Read More»

Genet. Mol. Res. 16(3): gmr16039074

DOI: 10.4238/gmr16039074

Human Genetics   Research Article

Clinical significance and expression of PUMA, MCL-1, and p53 in human renal cell carcinoma and para-carcinoma tissues

Authors: H.B. Xia, H.W. Cui, L. Su, Z.H. Zhang, X.Y. Yang, S.Q. Ning and X.L. Su

We investigated the expression level of p53 upregulated modulator of apoptosis (PUMA), myeloid cell leukemia-I (MCL-1), and p53 in renal cell carcinoma (RCC) and para-carcinoma tissues, as well as their clinical significance. The expression levels of PUMA, MCL-1, and p53 in RCC and para-carcinoma tissues were measured using immunohistochemical and quantitative real-.. Read More»

Genet. Mol. Res. 16(3): gmr16039278

DOI: 10.4238/gmr16039278

Plant Genetics   Research Article

Selection of maize inbred lines and gene expression for resistance to ear rot

Authors: G.S. Pereira, R.G.V. Pinho, E.V.R.V. Pinho, L.P.M. Pires, L.A.Y. Bernardo Junior, J.L.A. Pereira and M.P. Melo

In recent years, there has been a large incidence of fungi causing “ear rot” in maize in Brazil, the main fungus being Fusarium verticillioides. The most efficient and competitive alternative for control of this disease consists of using maize hybrids resistant to this pathogen. Thus, the aims of this study were to analyze the genetic variability of maiz.. Read More»

Genet. Mol. Res. 16(3): gmr16039415

DOI: 10.4238/gmr16039415

Human Genetics   Research Article

Improvement in orthopedic outcome score and reduction in IL-1β, CXCL13, and TNF-α in synovial fluid of osteoarthritis patients following arthroscopic knee surgery

Authors: Z.M. Li and M. Li

Knee osteoarthritis (KOA) is a common degenerative joint disease and immune system dysregulation has been implicated in its pathogenesis. The effect of arthroscopic knee surgery, an important method for KOA diagnosis and treatment, on the expression of immune regulators remains poorly understood. We aimed to investigate the clinical efficacy of such surgery and its .. Read More»

Genet. Mol. Res. 16(3): gmr16039487

DOI: 10.4238/gmr16039487

Animal Genetics   Research Article

Bayesian analyses of genetic parameters for growth traits in Nellore cattle raised on pasture

Authors: F.B. Lopes, J.L. Ferreira, R.B. Lobo and G.J.M. Rosa

This study was carried out to investigate (co)variance components and genetic parameters for growth traits in beef cattle using a multi-trait model by Bayesian methods. Genetic and residual (co)variances and parameters were estimated for weights at standard ages of 120 (W120), 210 (W210), 365 (W365), and 450 days (W450), and for pre- and post-weaning daily weight ga.. Read More»

Genet. Mol. Res. 16(3): gmr16039606

DOI: 10.4238/gmr16039606

Human Genetics   Research Article

Molecular analysis of the GSTT1 gene polymorphism in patients with clinical manifestation of atherosclerosis

Authors: J.V.M. Martins, D.A. Rodrigues, K.S.F. Silva, I.R. Costa, M.H. Lagares, F.L. Campedelli, A.M. Barbosa, M.P. Morais and K.K.V.O. Moura

Atherosclerosis is a chronic inflammatory disease formed by the accumulation of lipids in the innermost layer and large-caliber artery (tunica intima). This accumulation, along with platelet factors, stimulates the proliferation of muscle cells in this region. Over than 400 genes may be related to the pathology since they regulate endothelial function, coagulation, .. Read More»

Genet. Mol. Res. 16(3): gmr16039620

DOI: 10.4238/gmr16039620

Animal Genetics   Research Article

Alteration of -656(G/T) and -607(C/A) polymorphisms in interleukin-18 (IL-18) gene in house dust mite-sensitive allergic rhinitis patients in Thailand

Authors: A. Tungtrongchitr, J. Jumpasri, N. Sookrung, N. Visitsunthorn, P. Tantilipikorn, O. Piboonpocanan, N. Indrawattana, R. Tungtrongchitr and W. Chaicumpa

Allergic rhinitis (AR) is an IgE-mediated inflammation of the nasal membranes, which is naturally triggered by aeroallergens. House dust mites (HDM) are the most common inhalant allergens. Interleukin-18 (IL-18) has been established as an essential cytokine that can activate the generation of IgE. This randomized controlled study aimed to identify the possible relat.. Read More»

Genet. Mol. Res. 16(3): gmr16039641

DOI: 10.4238/gmr16039641

Human Genetics   Research Article

Mitochondrial DNA control region analysis of three ethnic populations in lower Northern part of Thailand

Authors: U. Suyasunanont, M. Nakkuntod and S. Mirasena

The lower northern part of Thailand contains various genetically diverse ethnic populations. The sequences of the mitochondrial DNA hypervariable region were studied in three ethnic populations inhabiting Phitsanulok Province. One hundred and nine nucleotide sequences - 53, 29, and 27 from Hmongs (Hill tribe), Lao Songs, and Thai-Siams, respectively - were collected.. Read More»

Genet. Mol. Res. 16(3): gmr16039687

DOI: 10.4238/gmr16039687

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