Lentinan depresses 3T3-L1 fat cell formation by inhibiting PPARγ/AKT signaling pathway
“Lentinan depresses 3T3-L1 fat cell formation by inhibiting PPARγ/AKT signaling pathway”, vol. 14, pp. 8084-8090, 2015.
, We investigated the mechanism of the effect of lentinan on 3T3-L1 fat cell formation by inhibiting the peroxisome proliferator-activated receptor gamma (PPARγ)/protein kinase B (AKT) signaling pathway. 3T3-L1 fat cells were treated with 80 mM lentinan with or without the PPARγ activator, 100 mM rosiglitazone for 24 h. Reverse transcription-polymerase chain reaction was applied to detect PPARγ and AKT mRNA expression levels. Western blotting was used to detect AKT protein expression level.