454 Pyrosequencing

Composition and structure of microbial communities associated with different domestic sewage outfalls

Z. H. Wang, Yang, J. Q., Zhang, D. J., Zhou, J., Zhang, C. D., Su, X. R., and Li, T. W., Composition and structure of microbial communities associated with different domestic sewage outfalls, vol. 13, pp. 7542-7552, 2014.

The diversity of microbiota in waste waters has not been thoroughly examined, despite the potential impact of microbes on effluent quality. Wastewater microbial communities harbor pathogenic bacteria, viruses, and parasites. To study microbial communities in domestic sewage outfalls, 454 pyrosequencing technology was used to investigate the composition of microbial communities associated with municipal wastewater during different seasons sampled over the course of one year. A total of 195,103 16S rRNA gene sequences were obtained from 20 samples.

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