454 Sequencing

New microsatellite markers for the abalone Haliotis midae developed by 454 pyrosequencing and in silico analyses

R. Slabbert, Hepple, J. - A., Rhode, C., Van der Merwe, A. E. Bester-, and Roodt-Wilding, R., New microsatellite markers for the abalone Haliotis midae developed by 454 pyrosequencing and in silico analyses, vol. 11, pp. 2769-2779, 2012.

Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enriched genomic libraries and high-throughput pyrosequencing technology.

Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification

T. O. Brunes, van de Vliet, M. S., Lopes, S., Alexandrino, J., Haddad, C. F. B., and Sequeira, F., Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification, vol. 12. pp. 242-247, 2013.

Twelve polymorphic tetranucleotide microsatellite loci were isolated and characterized for the leaf-frog Phyllomedusa burmeisteri, an endemic species of the Brazilian Atlantic forest. These loci were screened in 25 individuals from two populations of the Minas Gerais State (Carangola and Juiz de Fora). The number of alleles per locus ranged from 3 to 16 (mean = 8). Observed and expected heterozygosities ranged from 0.25 to 0.92 and 0.56 to 0.92, respectively.

Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing

L. A. Fusinatto, Lopes, S., Silva-Ferreira, A., Alexandrino, J., Haddad, C. F. B., Rocha, C. F. D., and Sequeira, F., Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing, vol. 12. pp. 230-234, 2013.

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population (Reserva Ecológica de Guapiaçu, REGUA) located in the central region of the Rio de Janeiro State, in Brazil. The mean number of alleles per locus ranged from 3 to 12.

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