Acidic extracellular chitinase

Isolation, partial characterization, and cloning of an extracellular chitinase from the entomopathogenic fungus Verticillium lecanii

G. Yu, Xie, L. Q., Li, J. T., Sun, X. H., Zhang, H., Du, Q., Li, Q. Y., Zhang, S. H., and Pan, H. Y., Isolation, partial characterization, and cloning of an extracellular chitinase from the entomopathogenic fungus Verticillium lecanii, vol. 14, pp. 2275-2289, 2015.

The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent of fungal phytopathogens, as well as insect pests. A 42-kDa chitinase belonging to family 18 of the glycosyl hydrolases was isolated and partially characterized. Chitinase was purified using successive column chromatography on phenyl-sepharose, DEAE-sepharose, and CM-sepharose. The enzyme showed the highest activity at 40°C and pH 4.6. Enzyme activity was strongly activated in the presence of Mg2+.

Subscribe to Acidic extracellular chitinase