AFLP

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

H. Y. Ma, Li, S. J., Feng, N. N., Ma, C. Y., Wang, W., Chen, W., Ma, L. B., Ma, H. Y., Li, S. J., Feng, N. N., Ma, C. Y., Wang, W., Chen, W., and Ma, L. B., “First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers”, vol. 15, p. -, 2016.

The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 triplets and 9 doublets. A total of 212 molecular markers were mapped, including 60 microsatellites and 152 AFLP markers. The linkage groups ranged from 7 to 102.5 cM and covered 2746.4 cM in length.

Genetic differentiation of Octopus minor (Mollusca, Cephalopoda) off the northern coast of China as revealed by amplified fragment length polymorphisms

J. M. Yang, Sun, G. H., Zheng, X. D., Ren, L. H., Wang, W. J., Li, G. R., and Sun, B. C., “Genetic differentiation of Octopus minor (Mollusca, Cephalopoda) off the northern coast of China as revealed by amplified fragment length polymorphisms”, vol. 14, pp. 15616-15623, 2015.

Octopus minor (Sasaki, 1920) is an economically impor­tant cephalopod that is found in the northern coastal waters of China. In this study, we investigated genetic differentiation in fishery populations using amplified fragment length polymorphisms (AFLPs). A total of 150 individuals were collected from five locations: Dalian (DL), Yan­tai (YT), Qingdao (QD), Lianyungang (LY), and Zhoushan (ZS), and 243 reproducible bands were amplified using five AFLP primer com­binations. The percentage of polymorphic bands ranged from 53.33 to 76.08%.

Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome

E. R. Monteiro, Strioto, D. K., Meirelles, A. C. S., Mangolin, C. A., and Machado, M. F. P. S., “Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome”, vol. 14, pp. 16966-16974, 2015.

Amplified fragment length polymorphism (AFLP) analysis was used to evaluate DNA polymorphism in Pilosocereus gounellei with the aim of differentiating samples grown in different Brazilian semiarid regions. Seven primer pairs were used to amplify 703 AFLP markers, of which 700 (99.21%) markers were polymorphic. The percentage of polymorphic markers ranged from 95.3% for the primer combination E-AAG/M-CTT to 100% for E-ACC/M-CAT, E-ACC/M-CAA, E-AGC/M-CAG, E-ACT/M-CTA, and E-AGG/M-CTG.

Genetic variation in wild populations of the tuber crop Amorphophallus konjac (Araceae) in central China as revealed by AFLP markers

C. Pan, Gichira, A. W., and Chen, J. M., “Genetic variation in wild populations of the tuber crop Amorphophallus konjac (Araceae) in central China as revealed by AFLP markers”, vol. 14, pp. 18753-18763, 2015.

Amorphophallus konjac is an economically important crop. In order to provide baseline information for sustainable development and conservation of the wild plant resources of A. konjac, we studied the genetic diversity and population structure of this species using amplified fragment length polymorphism (AFLP) molecular markers. We sampled 139 individuals from 10 wild populations of A. konjac in central China. Using five AFLP primer combinations, we scored a total of 270 DNA fragments, most of which were polymorphic (98.2%).

Molecular genetic analysis of heterosis in interspecific hybrids of Argopecten purpuratus x A. irradians irradians

L. P. Hu, Huang, X. T., Sun, Y., Mao, J. X., Wang, S., Wang, C. D., and Bao, Z. M., “Molecular genetic analysis of heterosis in interspecific hybrids of Argopecten purpuratus x A. irradians irradians”, vol. 14, pp. 10692-10704, 2015.

Argopecten purpuratus and Argopecten irradians irradi­ans hybridization was successfully performed and the hybrid offspring displayed apparent heterosis in growth traits. To better understand the genetic basis of heterosis, the genomic composition and genetic varia­tion of the hybrids were analyzed with amplified fragment length poly­morphism (AFLP) and simple sequence repeat (SSR) markers. Seven of eight universal SSR primers displayed polymorphism in the hybrids and their parental groups, and hybrids inherited both parental geno­types at each locus.

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