Agrobacterium tumefaciens

Improving plant transformation using Agrobacterium tumefaciens

L. V. Ribeiro Neto, Oliveira, A. P., Lourenço, M. V., Bertoni, B. W., França, S. C., Rosa-Santos, T. M., and Zingaretti, S. M., Improving plant transformation using Agrobacterium tumefaciens, vol. 14, pp. 6695-6698, 2015.

Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in Eclipta alba explants.

An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.)

H. J. Zhang, Gao, P., Wang, X. Z., and Luan, F. S., An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.), vol. 13, pp. 54-63, 2014.

An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. The new Agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. The transformation system was maximized to maintain the integrity of melon itself, thus avoiding the limitations of traditional tissue culture methods. The transformation was carried out under a non-sterile environment.

Efficient production of transgenic melon via Agrobacterium-mediated transformation

I. Bezirganoglu, Hwang, S. Y., Shaw, J. F., and Fang, T. J., Efficient production of transgenic melon via Agrobacterium-mediated transformation, vol. 13, pp. 3218-3227, 2014.

Oriental melon (Cucumis melo L. var. makuwa) is an important fruit for human consumption. However, this plant species is one of the most recalcitrant to genetic transformation. The lack of an efficient in vitro system limits the development of a reproducible genetic transformation protocol for Oriental melon. In this study, an efficient transgenic production method for Agrobacterium-mediated transformation using cotyledon explants of Oriental melon was developed.

Improved production of transgenic Dioscorea zingiberensis (Dioscoreaceae) by Agrobacterium tumefaciens-mediated transformation

L. Shi, Fan, J. Q., Hu, C. G., Luo, J., and Yao, J. L., Improved production of transgenic Dioscorea zingiberensis (Dioscoreaceae) by Agrobacterium tumefaciens-mediated transformation, vol. 11, pp. 244-253, 2012.

The establishment of high-efficiency Agrobacterium-mediated transformation techniques could improve the production of Dioscorea zingiberensis, a medicinal species with a high diosgenin content. We co-cultivated embryogenic calli induced from mature seeds with A. tumefaciens strain EHA105. A binary vector, pCAMBIA1381, which contains the gfp and hpt genes under the control of the ubiquitin promoter and the CaMV 35S promoter, respectively, was used for transformation.

Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

L. Liu, Wei, Y. M., Zhou, X. W., Lin, J., Sun, X. F., and Tang, K. X., Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21, vol. 12, pp. 2913-2922, 2013.

An efficient Agrobacterium tumefaciens-mediated ge­netic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hy­gromycin B resistance expression vector, pCAMBIA1304’AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and opti­mized. PCR amplification and Southern hybridization were used to verify the transformation events.

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