Allele frequency

Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goiás, Central Brazil

R. G. Rodovalho, Santos, G. S., Cavalcanti, L. M., Moura, B. F. S. M., Rodrigues, E. L., Lima, P. R., Gigonzac, M. A. D., and Vieira, T. C., Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goiás, Central Brazil, vol. 14, pp. 7310-7314, 2015.

In studies of human identification, obtaining a high standard of outcomes and satisfactory conclusions are directly related to the use of highly polymorphic molecular markers. In addition to the combined DNA index system (CODIS) group, it is also important to implement non-CODIS markers into the analysis, as they increase the power of discrimination. During the identification process, it is essential to consider the genetic variation among distinct groups of populations, as the allele frequencies are directly associated with the power of discrimination.

Usefulness of direct sequencing of pooled DNA for SNP identification and allele-frequency determination compatible with a common disease/common variant hypothesis

M. K. Kim, Nam, T. S., Choi, K. H., Jang, S. Y., Kim, Y. O., and Lee, M. C., Usefulness of direct sequencing of pooled DNA for SNP identification and allele-frequency determination compatible with a common disease/common variant hypothesis, vol. 9, pp. 772-779, 2010.

We examined the efficiency of direct sequencing of pooled DNA for developing common single nucleotide polymorphisms (SNPs) and its accuracy for estimating allele frequencies. A pool of 200 control DNAs was established and was used for developing SNPs and estimating minor allele frequencies (MAF). The sensitivity of the pooled DNA method for successfully detecting an SNP with an MAF >0.01 listed in the database was approximately 0.7; it was particularly efficient for detecting SNPs with MAF >0.1, which is compatible with the common disease/common variant hypothesis.

Genetic analysis of 30 InDel markers for forensic use in five different Chinese populations

C. T. Li, Zhang, S. H., and Zhao, S. M., Genetic analysis of 30 InDel markers for forensic use in five different Chinese populations, vol. 10, pp. 964-979, 2011.

Allele frequencies of 30 insertion/deletion polymorphism (InDel) markers previously selected and validated for forensic purposes were assessed in 419 unrelated individuals originating from five different populations of P.R. China, including Chinese Han, Chinese Hui, Uighur, Mongolians, and Tibetans.

Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations

Z. L. Xu, Ali, Z., Yi, J. X., He, X. L., Zhang, D. Y., Yu, G. H., Khan, A. A., Khan, I. A., and Ma, H. X., Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations, vol. 10, pp. 1262-1276, 2011.

Salicornia spp is one of the most salt-tolerant vascular plants and is native to salt marshes and estuaries. We developed expressed sequence tag derived-simple sequence repeat (EST-SSR) markers for estimating genetic diversity and marker-assisted Salicornia breeding. Six polymorphic EST-SSRs of 40 detected 27 alleles, ranging from three to five alleles per locus. The average number of alleles per locus was 4.33 and 4.17, and the major allele frequency at locus DY529765 was high, being 0.859 and 0.857 in S. bigelovii and S. europea, respectively.

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