Amazon region

Genetic differences based on AFLP markers in the mosquito species Anopheles darlingi collected in versus near houses in the region of Porto Velho, RO, Brazil

L. M. Santos, Gama, R. A., Eiras, A. E., and Fonseca, C. G., “Genetic differences based on AFLP markers in the mosquito species Anopheles darlingi collected in versus near houses in the region of Porto Velho, RO, Brazil”, vol. 9, pp. 2254-2262, 2010.

Anopheles darlingi is the most important malaria vector in Central and South America. After a dramatic reduction of malaria cases in the whole Brazilian territory, with the lowest abundance being attained by 1970, the disease resurged in the Amazon region, where it is now a great public health concern. Consequently, better knowledge about vector species became urgent. We examined the genetic diversity and population structure of A. darlingi, sampled in four localities in the State of Rondônia, Brazil, using 139 amplified fragment length polymorphism marker loci.

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

M. M. Castro, Farias, I. P., Borborema-Santos, C. M., Correia, G., and Astolfi-Filho, S., “Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region”, vol. 10, pp. 186-196, 2011.

Infection by human papillomavirus (HPV) is one of the primary causes of mortality by cancer in northern Brazil. Sexually active women from Manaus, Amazonas, without cytological alterations and women with pre-malignant and malignant cytological alterations were examined for HPV virus, identified via PCR and sequencing. The target region for this study was part of the L1 capsid gene of HPV. Twenty-three samples that were PCR-positive were sequenced.

Karyotype of Brazilian Anopheles albitarsis sensu lato (Diptera:Culicidae)

M. Silva Rafael, Santos-Junior, I. Pereira do, Tadei, W. Pedro, Sallum, M. Anice Mure, and Forattini, O. Paulo, “Karyotype of Brazilian Anopheles albitarsis sensu lato (Diptera:Culicidae)”, vol. 4, pp. 684-690, 2005.

Anopheles (Nyssorhynchus) albitarsis sensu lato is an important malaria vector in Brazil, especially in the Brazilian Amazon region. Chromosome preparations of fourth-instar larvae of A. albitarsis from Iranduba and Coari (AM) and Ilha Comprida (SP) were analyzed for karyotype determination and to improve cytogenetic identification of this species. Anopheles albitarsis possesses 2n = 6 chromosomes, with two pairs (submetacentric and metacentric) of autosomes and one pair of sex chromosomes, with X-Y dimorphism.

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