Amniotic fluid

Cryopreservation does not alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid mesenchymal stem cells

P. C. Ângelo, Ferreira, A. C. S., Fonseca, V. D., Frade, S. P., Ferreira, C. S., Malta, F. S. V., Pereira, A. K., Leite, H. V., Brum, A. P., Pardini, V. C., Gomes, K. B., and Cabral, A. C. V., Cryopreservation does not alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid mesenchymal stem cells, vol. 11, pp. 1002-1012, 2012.

Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed chromosomal stability through karyotype analysis, determined whether multipotent capacity (differentiation into adipogenic, chondrogenic, and osteogenic cells) is maintained, and analyzed SOX2 and NANOG expression after thawing.

Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid

M. Alam, Mahajan, M., Raziuddin, M., Singh, T. P., and Yadav, S., Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid, vol. 8, pp. 929-937, 2009.

Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chroma­tography. The protein had earlier been reported to be serendipitously purified along with PON1 (paraoxonase 1).

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