Aneuploidy

Loss of STAG2 causes aneuploidy in normal human bladder cells

X. Li, Zhang, T. W., Tang, J. L., Fa, P. P., Lu, J. X., Qi, F. M., Cai, Z. M., Liu, C. X., and Sun, X. J., “Loss of STAG2 causes aneuploidy in normal human bladder cells”, vol. 14, pp. 2638-2646, 2015.

The aim of this study was to determine how the function of human stromal antigen 2 (STAG2) plays an important role in proper chromosome separation. STAG2 mRNA in normal bladder cells and bladder tumor cells was evaluated by RT-PCR. The protein levels of STAG2 in normal bladder cells and bladder tumor cells were determined by western blot. A cell proliferation assay was used to measure the growth of tumor cells and STAG2-inhibited normal cells, and STAG2- inhibited normal cells were subjected to karyotype analysis.

Apomixis in cassava: advances and challenges

D. Y. H. Freitas and Nassar, N. M. A., “Apomixis in cassava: advances and challenges”, vol. 12. pp. 988-994, 2013.

Cassava is the most important staple crop in the Tropics and Subtropics. Apomixis may revolutionize its production due to various attributes. These potential advantages include production by true seed, maintaining cultivar superiority over generations without segregation, and avoiding contamination by bacteria and viruses. Historically, apomixis was initially observed by International Institute of Tropical Agriculture researchers, in the 1980s, in homogenous progeny of hybrid crosses.

Validation of quantitative fluorescent-PCR for rapid prenatal diagnosis of common aneuploidies in the Chinese population

A. - Q. Xu, Xia, M., Liu, J. - T., Yao, F. - X., Zhang, W. - M., Hao, N., Zhou, J., and Bian, X. - M., “Validation of quantitative fluorescent-PCR for rapid prenatal diagnosis of common aneuploidies in the Chinese population”, vol. 12, pp. 6379-6388, 2013.

Quantitative fluorescent polymerase chain reaction (QF-PCR) is an accurate and reliable method for rapid detection of aneuploidy; however, it is not routinely used in China. We aimed to validate QF-PCR as a means for prenatal common aneuploidy screening and to analyze the heterozygosities of short tandem repeat (STR) markers in the Chinese population. The sequences of 19 STR markers in chromosomes 21, 18, 13, X, and Y were designed; three kinds of fluoresceins were used to label the primers, and the QF-PCR detecting conditions were explored and optimized.

Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny

N. M. A. Nassar, Gomes, P. T. C., Chaib, A. M., Bomfim, N. N., Batista, R. C. D., and Collevatti, R. G., “Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny”, vol. 8, pp. 1323-1330, 2009.

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical with each other and with their maternal progenitor. Sibs of selfed M. glaziovii proved to be identical when examined with these microsatellite loci.

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