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Age-related changes in mitochondrial UCP, ANT and COX III gene expression in the breast muscle of quails (Coturnix coturnix japonica)

E. Gasparino, Del Vesco, A. P., Guimarães, S. E. F., da Silva, S. C. C., Marcato, S. M., Neto, A. Rde Oliveir, and Sousa, K. R. S., Age-related changes in mitochondrial UCP, ANT and COX III gene expression in the breast muscle of quails (Coturnix coturnix japonica), vol. 11, pp. 1981-1989, 2012.

Efficient conversion of food into body mass has been associated with altered gene expression of some proteins of the electron transport chain. We evaluated the effect of age on mRNA expression of uncoupling protein, adenine nucleotide translocase and cytochrome c oxidase complex III in quails. One-day-old broiler quails were sacrificed after 7, 14, 21, or 28 days. Six quails of each age were slaughtered by cervical dislocation. Total RNA was extracted from the breast muscle and reverse transcribed into cDNA. Real-time PCR analysis was performed using specific primers for the genes.

Macromolecular array patterns of silk gland secretion in social Hymenoptera larvae

G. Gomes, Silva-Zacarin, E. C. M., de Moraes, R. L. M. Silva, Caetano, F. H., and Zara, F. J., Macromolecular array patterns of silk gland secretion in social Hymenoptera larvae, vol. 3, pp. 309-322, 2004.

The cocoon, produced by most holometabolous insects, is built with silk that is usually produced by the larval salivary gland. Although this silk has been widely studied in the Lepidoptera, its composition and macromolecular arrangement remains unknown in the Hymenoptera. The macromolecular array patterns of the silk in the larval salivary gland of some meliponids, wasps, and ants were analyzed with polarized-light microscopy, and they were compared with those of Bombyx mori (Lepidoptera).

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