Anthocyanin

Cloning of an anthocyanidin synthase gene homolog from blackcurrant (Ribes nigrum L.) and its expression at different fruit stages

X. - G. Li, Wang, J., and Yu, Z. - Y., Cloning of an anthocyanidin synthase gene homolog from blackcurrant (Ribes nigrum L.) and its expression at different fruit stages, vol. 14, pp. 2726-2734, 2015.

Anthocyanidin synthase (ANS), a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. In this study, a full-length, 1427-bp long cDNA named RnANS1, which is homologous to the anthocyanidin synthase gene, was cloned from blackcurrant using a homologous cloning strategy. RnANS1 is highly homologous to other plant ANS genes at both the nucleotide and amino acid sequence levels.

A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.)

J. W. Wang, Wang, H. Q., Xiang, W. W., and Chai, T. Y., A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.), vol. 13, pp. 3615-3626, 2014.

We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructed, which localized to the plasma membrane of onion epidermal cells.

 Cloning and expression of UDP-glucose: flavonoid 3-O-glucosyltransferase gene in peach flowers

X. C. Wen, Han, J., Leng, X. P., Ma, R. J., Jiang, W. B., and Fang, J. G.,  Cloning and expression of UDP-glucose: flavonoid 3-O-glucosyltransferase gene in peach flowers, vol. 13, pp. 10067-10075, 2014.

To elucidate the connection between flower coloration and the expression of genes associated with anthocyanin biosynthesis, a gene encoding UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) was isolated, and the expression of the last four genes in the anthocyanin biosynthetic pathway during peach flower development was determined. The nucleotide sequence of the peach UFGT (GenBank accession No. JX149550) is highly similar to its homologs in other plants.

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

J. Kumchai, Huang, J. - Z., Lee, C. - Y., Chen, F. - C., and Chin, S. - W., Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro, vol. 12, pp. 5589-5601, 2013.

In vitro grown cabbage (Brassica oleracea var. capitata) seedlings exposed to excess molybdenum (Mo) ions exhibited severely reduced plant growth at the cotyledonary stage. Adding 80 mM proline (Pro) to the Mo-treated medium could help 50% seedlings to overcome the toxicity and grow true leaves. Under excess Mo stress, seedlings accumulated blue/purple anthocyanin in their cotyledons and hypocotyls. The anthocyanin content under Mo with 40 mM Pro was 4-fold higher than the control medium, MS with 40 mM Pro.

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