Apis cerana

Genomic characterization and phylogenetic analysis of Chinese sacbrood virus isolated from Loess Plateau, China

H. Yu, Liu, T. X., Wang, D., Yu, H., Liu, T. X., and Wang, D., “Genomic characterization and phylogenetic analysis of Chinese sacbrood virus isolated from Loess Plateau, China”, vol. 15, p. -, 2016.

The complete genomic RNA of the Chinese sacbrood virus (CSBV) strain, which infects the honeybees in the Loess plateau, was sequenced and analyzed. The CSBV-SX strain contains 8705 nucleotides, which includes a single large open reading frame (99-8681 nucleotides) encoding 2860 amino acids. A novel efficient identification method was used to investigate the samples infected by CSBV.

Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana)

T. Ji, Shen, F., Liu, Z., Yin, L., Shen, J., Liang, Q., and Luo, Y. X., “Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana)”, vol. 14, pp. 10103-10118, 2015.

The mite (Varroa destructor) has become the greatest threat to apiculture worldwide. As the original host of the mite, Apis cerana can effectively resist the mite. An increased understanding of the resistance mechanisms of Eastern honeybees against V. destructor may help researchers to protect other species against these parasites. In this study, the proteomes of 4 Apis cerana colonies were analyzed using an isobaric tag for relative and absolute quantitation technology.

High-throughput sequencing identification of genes involved with Varroa destructor resistance in the eastern honeybee, Apis cerana

T. Ji, Yin, L., Liu, Z., Shen, F., and Shen, J., “High-throughput sequencing identification of genes involved with Varroa destructor resistance in the eastern honeybee, Apis cerana”, vol. 13, pp. 9086-9096, 2014.

Varroa destructor is the greatest threat to the honeybee Apis mellifera worldwide, while it rarely causes serious harm to its native host, the Eastern honeybee Apis cerana. The genetic mechanisms underlying the resistance of A. cerana to Varroa remain unclear. Thus, understanding the molecular mechanism of resistance to Varroa may provide useful insights for reducing this disease in other organisms. In this study, the transcriptomes of two A. cerana colonies were sequenced using the Illumina Solexa sequencing method.

Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor

T. Ji, Yin, L., Liu, Z., Liang, Q., Luo, Y., Shen, J., and Shen, F., “Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor”, vol. 13, pp. 8888-8900, 2014.

The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage to its native host Apis cerana. Understanding the resistance mechanisms of eastern bees against Varroa mites will help researchers determine how to protect other species from this organism. The A. cerana genome has not been previously sequenced; hence, here we sequenced the A. cerana nurse workers transcriptome and monitored the differential gene expression of A. cerana bees challenged by V.

Genetic diversity of the honeybee Apis cerana in Yunnan, China, based on mitochondrial DNA

L. Yin and Ji, T., “Genetic diversity of the honeybee Apis cerana in Yunnan, China, based on mitochondrial DNA”, vol. 12, pp. 2002-2009, 2013.

DNA sequence diversity in the tRNAleu-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNAleu-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 were new. The mean diversity of haplotypes was 0.752 ± 0.030 (0.378-0.698), and nucleotide diversity was 0.01073 ± 0.00087 (0.00412-0.01123).

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