Apostichopus japonicus

Development and characterization of 70 novel microsatellite markers for the sea cucumber (Apostichopus japonicus)

W. Peng, Bao, Z. M., Du, H. X., Yan, J. J., Zhang, L. L., and Hu, J. J., “Development and characterization of 70 novel microsatellite markers for the sea cucumber (Apostichopus japonicus)”, vol. 11, pp. 434-439, 2012.

The sea cucumber (Apostichopus japonicus) is an important item in Asian cuisine. It is currently produced through aquaculture, especially in China, after being overexploited in the wild in the 1990s. We isolated 70 novel polymorphic microsatellite loci using an enrichment-colony hybridization protocol. All loci were characterized in 48 individuals from a natural population in Rongcheng (Shandong, China) using genomic DNA isolated from muscle tissue.

Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus

L. Zhu, Li, C. H., Su, X. R., Guo, C. Y., Wang, Z., Jin, C. H., Li, Y., and Li, T. W., “Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus”, vol. 12, pp. 3028-3037, 2013.

Rapid and efficient growth is a major consideration and challenge for global mariculture. The differential growth rate of the sea cucumber, Apostichopus japonicus, has significantly hampered the total production of the industry. In the present study, forward and reverse suppression subtractive hybridization libraries were constructed and sequenced from a fast-growth group and a slow-growth group of the sea cucumber. A total of 142 differentially expressed sequence tags (ESTs) with insertions longer than 150 bp were identified and further analyzed.

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