Aptamer

Bottom-up assembly of RNA nanoparticles containing phi29 motor pRNA to silence the asthma STAT5b gene

C. Qiu, Peng, W. K., Shi, F., and Zhang, T., Bottom-up assembly of RNA nanoparticles containing phi29 motor pRNA to silence the asthma STAT5b gene, vol. 11, pp. 3236-3245, 2012.

Activation of the transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key event in the development of asthma. The potent ability of small interfering RNA (siRNA) to inhibit the expression of STAT5b mRNA has provided a new class of therapeutics for asthma. However, efficient delivery of siRNAs remains a key obstacle to their successful application. A targeted intracellular delivery approach for siRNA to specific cell types would be highly desirable.

Screening and identification of the nucleic acid aptamers in nasopharyngeal carcinoma

W. - X. Chen, Zhang, K. - H., Zou, X. - S., Chen, Y. - Q., and Li, J. - G., Screening and identification of the nucleic acid aptamers in nasopharyngeal carcinoma, vol. 12, pp. 6850-6857, 2013.

To screen the nucleic acid aptamers of the EB virus-positive nasopharyngeal carcinoma cells, we used SELEX technology and synthesized in vitro a 78-nucleotide random DNA library. We used normal nasopharyngeal epithelial cells and EB virus-positive low differentiated nasopharyngeal carcinoma cells as target to conduct 10 cycles of screening, cloning, sequencing, and identification of the aptamers.

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