Assisted reproduction technology

Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells

L. T. Iguma, Iguma, L. T., Melo, E. O., Franco, M. M., Pivato, I., Vianna, G. R., Sousa, R. V., Dode, M. A. N., Aragão, F. J. L., Rech, E. L., Rech, E. L., Rumpf, R., and Lisauskas, S. F. C., Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells, vol. 4, pp. 55-66, 2005.

An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capacities of NT using fibroblast cells isolated from a 14-month-old cloned Simmental heifer (FCE) vs the same line transfected with a plasmid containing neomycin-resistant genes (TFCE).

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