Asthma

Polymorphisms in the vascular endothelial growth factor (VEGF) gene associated with asthma

H. Y. Lu, Zhao, G. L., Fu, M. F., Lu, H. Y., Zhao, G. L., and Fu, M. F., “Polymorphisms in the vascular endothelial growth factor (VEGF) gene associated with asthma”, vol. 15, p. -, 2016.

It has been proven that vascular endothelial growth factor (VEGF) is involved in airway restoration and the development of asthma. We sought to examine the relevance of VEGF gene polymorphisms to asthma in the Chinese Han population. We extracted the whole genomic DNA from the peripheral blood of 471 participants, including 226 patients with asthma and 245 healthy controls. Seven single nucleotide polymorphisms (SNPs) of the VEGF gene were genotyped using the MassARRAY system. The data were then analyzed using HaploView 4.0 and SPSS 20.0 softwares.

Correlation between polymorphisms in the glucocorticoid receptor gene NR3C1 and susceptibility to asthma in a Chinese population from the Henan Province

Z. Cheng, Dai, L. L., Liu, Q., Liu, M., Wang, Q., Li, P. F., Wang, H., Jia, L. Q., An, L., Cheng, Z., Dai, L. L., Liu, Q., Liu, M., Wang, Q., Li, P. F., Wang, H., Jia, L. Q., and An, L., “Correlation between polymorphisms in the glucocorticoid receptor gene NR3C1 and susceptibility to asthma in a Chinese population from the Henan Province”, vol. 15, p. -, 2016.

The aim of this study was to investigate the association between four single nucleotide polymorphisms in NR3C1 (Tth111I, BclI, ER22/23EK, and N363S), which encode the glucocorticoid receptor, and asthma susceptibility in patients from the Henan Province of China. Three hundred and twenty-eight patients with asthma and 60 healthy volunteers were recruited to this study. The target SNPs were genotyped by polymerase chain reaction (PCR)-high resolution melting and PCR-restriction fragment length polymorphism.

Boswellic acid attenuates asthma phenotype by downregulation of GATA3 via nhibition of PSTAT6

X. Zhou, Cai, J. G., Zhu, W. W., Zhao, H. Y., Wang, K., and Zhang, X. F., “Boswellic acid attenuates asthma phenotype by downregulation of GATA3 via nhibition of PSTAT6”, vol. 14, pp. 7463-7468, 2015.

To study the role of boswellic acid in reducing asthma phenotype severity and the relationship between the expression of pSTAT6 and GATA3, thirty-six mice were randomly divided into normal control group, asthma group, and boswellic acid group (treatment group). The asthma model was established through an intraperitoneal injection of sensitization liquid (0.15 mL aluminum hydroxide gel at 88.67 mg/mL and 0.05 mg ovalbumin). pSTAT6 and GATA3 expression levels in peripheral blood were detected by reverse transcription-polymerase chain reaction and Western blot analysis.

Construction of a lentiviral vector encoding heme oxygenase 1 and its introduction into mouse adipose tissue-derived stem cells

C. H. Zhu, Lei, W., and Chen, Z. R., “Construction of a lentiviral vector encoding heme oxygenase 1 and its introduction into mouse adipose tissue-derived stem cells”, vol. 14, pp. 10705-10716, 2015.

Many studies exist concerning the use of stem cells as delivery vehicles in gene therapy, expressing genes such as vascular endothelial growth factor 165 and hepatocyte growth factor. However, few reports regarding adipose tissue-derived stem cells (ADSCs) and the heme oxygenase 1 (HO-1) gene have been published. Therefore, we established a lentiviral vector encoding HO-1 and used this to infect ADSCs with the aim of producing therapeutic seed cells.

Subscribe to Asthma