Avascular necrosis of the femoral head

Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head

X. N. Wang, Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., Wang, J. C., Wang, X. N., Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., and Wang, J. C., Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head, vol. 15, p. -, 2016.

This study aimed to evaluate 12 genes (18S, GAPDH, B2M, ACTB, ALAS1, GUSB, HPRT1, PBGD, PPIA, PUM1, RPL29, and TBP) for their reliability and stability as reference sequences for real-time quantitative PCR (RT-qPCR) in bone marrow-derived mesenchymal stem cells (BMSCs) isolated from patients with avascular necrosis of the femoral head (ANFH). BMSCs were isolated from 20 ANFH patients divided into four groups according to etiology, and four donors with femoral neck fractures.

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