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Reference gene selection for gene expression studies in lily using quantitative real-time PCR

M. F. Zhang, Liu, Q., Jia, G. X., Zhang, M. F., Liu, Q., and Jia, G. X., Reference gene selection for gene expression studies in lily using quantitative real-time PCR, vol. 15, p. -, 2016.

Quantitative real-time polymerase chain reaction (qRT-PCR) is an important technology used to analyze gene-expression levels. Reference genes, which are assumed to be expressed consistently across various developmental stages and in different tissues, were selected for expression level analysis. Using digital gene expression technology, we selected nine reference genes (18S, EF, CYCOL, SAND, GAPDH, ACTIN, BHLH, TIP, and Clathrin) as candidate reference genes for further study.

Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head

X. N. Wang, Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., Wang, J. C., Wang, X. N., Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., and Wang, J. C., Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head, vol. 15, p. -, 2016.

This study aimed to evaluate 12 genes (18S, GAPDH, B2M, ACTB, ALAS1, GUSB, HPRT1, PBGD, PPIA, PUM1, RPL29, and TBP) for their reliability and stability as reference sequences for real-time quantitative PCR (RT-qPCR) in bone marrow-derived mesenchymal stem cells (BMSCs) isolated from patients with avascular necrosis of the femoral head (ANFH). BMSCs were isolated from 20 ANFH patients divided into four groups according to etiology, and four donors with femoral neck fractures.

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