Biofilm

Preliminary study on total protein extraction methods from Enterococcus faecalis biofilm

W. Chen, Liang, J., He, Z., Jiang, W., Chen, W., Liang, J., He, Z., and Jiang, W., Preliminary study on total protein extraction methods from Enterococcus faecalis biofilm, vol. 15, p. -, 2016.

Enterococcus faecalis is the major pathogen of post-endodontic disease and refractory periapical periodontitis, and recent research on this species has focused on its pathogenicity. E. faecalis most often causes disease in the form of a biofilm, and total protein expression shows a strong association with its virulence. Therefore, the purpose of our study was to explore different methods of extracting the total proteins of the E. faecalis (ATCC 33186 standard strain) biofilm.

Stimulation of bacterial biofilms on Th17 immune cells

G. Q. Wang, Wang, L., Zhang, H. L., Dong, Y. Q., and Yang, Y. X., Stimulation of bacterial biofilms on Th17 immune cells, vol. 14, pp. 7721-7726, 2015.

We investigated the role of bacterial biofilms in stimulating T helper 17 (Th17) cells in infected organisms. The formation of bacterial biofilms isolated from clinical lavage fluid samples was measured. Th17 cells and interleukin 17 (IL-17) levels in the peripheral blood of healthy individuals, people infected by biofilm bacteria, people infected by non-biofilm bacteria, and in the lavage fluid from people infected by bacteria were determined. Differences in those data were tested using the SPSS 17.0 statistical software.

GlpC gene is responsible for biofilm formation and defense against phagocytes and imparts tolerance to pH and organic solvents in Proteus vulgaris

Y. L. Wu, Liu, K. S., Yin, X. T., and Fei, R. M., GlpC gene is responsible for biofilm formation and defense against phagocytes and imparts tolerance to pH and organic solvents in Proteus vulgaris, vol. 14, pp. 10619-10629, 2015.

Biofilm-forming bacteria are highly resistant to antibiotics, host immune defenses, and other external conditions. The formation of biofilms plays a key role in colonization and infection. To explore the mechanism of biofilm formation, mutant strains of Proteus vulgaris XC 2 were generated by Tn5 random transposon insertion. Only one biofilm defective bacterial species was identified from among 500 mutants. Inactivation of the glpC gene coding an anaerobic glycerol-3-phosphate dehydrogenase subunit C was identified by sequence analysis of the biofilm defective strain.

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