Birds

Identification and characterization of polymorphic microsatellite loci in the red-crowned crane

L. Zhang, Zhang, Z. H., Shen, F. J., Hou, R., Zhang, W. P., Liu, Y. L., Tu, K. Y., and Yang, A. L., Identification and characterization of polymorphic microsatellite loci in the red-crowned crane, vol. 14, pp. 15169-15176, 2015.

We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes. The number of observed alleles ranged from 3 to 10. Observed and expected heterozygosities ranged from 0.197 to 0.935 and 0.453 to 0.887, respectively; the mean polymorphic information content was 0.663.

Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals

I. A. Arif, Khan, H. A., M. Sadoon, A., and Shobrak, M., Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals, vol. 10, pp. 3559-3564, 2011.

In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single-annealing protocol. The PCR success rate for the amplification of the mini-barcode region was: mammals (4/5), reptiles (5/11) and birds (4/7).

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