Bone marrow mesenchymal stem cells

Treating congenital megacolon by transplanting GDNF and GFRα-1 double genetically modified rat bone marrow mesenchymal stem cells

C. B. Zhou, Peng, C. H., Pang, W. B., Zhang, D., and Chen, Y. J., Treating congenital megacolon by transplanting GDNF and GFRα-1 double genetically modified rat bone marrow mesenchymal stem cells, vol. 14, pp. 9441-9451, 2015.

We studied the survival and gene expression of glial cell line-derived neurotrophic factor (GDNF) and GDNF receptor α-1 (GFRα-1) double-genetically modified rat bone marrow mesenchymal stem cells (BMSCs) transplanted into the intestinal walls of the rat models with congenital megacolon and determine the feasibility of treatment by transplantation of double-genetically modified rat BMSCs. The rat colorectal intestinal wall nerve plexus was treated with the cationic surface active agent benzalkonium chloride to establish an experimental megacolon model.

Impact of BMMSCs from different sources on proliferation of CD34+ cells

R. F. Liu, Li, J. Q., Hou, R. X., Wang, R., and Zhang, K. M., Impact of BMMSCs from different sources on proliferation of CD34+ cells, vol. 14, pp. 474-482, 2015.

There are significant differences on the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), immunological response, and antigen-presenting functions between patients with psoriasis and normal subjects, but there are no significant differences in aborted fetuses. We examined the differences in BMMSCs between aborted fetuses and patients with psoriasis in this study. Bone marrow from normal subjects, aborted fetuses, and patients with psoriasis were obtained using a MidiMACS machine.

Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells

Y. Xu, Zhang, X. J., Fang, L., and Zhao, T. B., Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells, vol. 14, pp. 3932-3938, 2015.

To investigate the effect on cell proliferation and extracellular matrix expression of annulus fibrosus (AF) cells when co-cultured with bone marrow mesenchymal stem cells (BMSCs). Primary isolated rabbit BMSCs and AF cells were cultured and harvested cells were placed into a 15-mL centrifugal tube co-culture system in a ratio of 2:1 (A group), 1:1 (B group), and 1:2 (C group). Cell proliferation was evaluated using cell counting kit-8, and mRNA of collagen II and mucopolysaccharide was quantified using real-time polymerase chain reaction (PCR) on Days 7, 14, and 21.

Immunomodulatory effect of bone marrow mesenchymal stem cells on T lymphocytes in patients with decompensated liver cirrhosis

C. H. Guo, Han, L. X., Wan, M. R., Deng, G. J., and Gan, J. H., Immunomodulatory effect of bone marrow mesenchymal stem cells on T lymphocytes in patients with decompensated liver cirrhosis, vol. 14, pp. 7039-7046, 2015.

We explored the immunomodulatory effects of bone marrow mesenchymal stem cells (BMSCs) on peripheral blood T lym­phocytes in patients with decompensation stage, hepatitis B-associated cirrhosis. MSCs from nine patients were analyzed by flow cytometry. Peripheral blood lymphocytes were isolated for fluorescent staining.

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

G. Wu, Cui, Y., Wang, Y. T., Yao, M., Hu, J., Li, J. X., Wang, Y., and Zhang, B., Repair of cartilage defects in BMSCs via CDMP1 gene transfection, vol. 13, pp. 291-301, 2014.

This study aimed at investigating the ability of cartilage-derived morphogenetic protein 1 (CDMP1) gene-transfected bone marrow mesenchymal stem cells (BMSCs) loaded on the poly(lactic-co-glycolic acid) (PLGA) scaffold for the repair of laryngeal cartilage defects and make a preliminary assessment of its repair effect. The mRNA and protein expressions of hCDMP1 were detected by reverse transcriptase-polymerase chain reaction and Western blotting. The expression of type II collagen (Col II) and glycosaminoglycan (GAG) were detected by immunohistochemistry.

Fibroin protein/chitosan scaffolds and bone marrow mesenchymal stem cells culture in vitro

J. Deng, She, R. F., Huang, W. L., Yuan, C., and Mo, G., Fibroin protein/chitosan scaffolds and bone marrow mesenchymal stem cells culture in vitro, vol. 13, pp. 5745-5753, 2014.

A previous experiment demonstrated that fibroin protein and chitosan mixed in proper proportion presented good physical and chemical properties and biological characteristics, which can make up for their respective disadvantages. To observe the growth of bone marrow mesenchymal stem cells (BMSCs) on these fibroin protein/chitosan 3D scaffolds, induced rabbit BMSCs were seeded on fibroin protein/chitosan scaffolds. The cell adhesion rate was measured, and cell growth was observed under an inverted microscope and a scanning electron microscope.

Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits

P. - F. Liu, Guo, L., Zhao, D. - W., Zhang, Z. - J., Kang, K., Zhu, R. - P., and Yuan, X. - L., Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits, vol. 13, pp. 7992-8001, 2014.

The aim of this study was to investigate the repair effect of human acellular amniotic membrane (HAAM) loading bone marrow mesenchymal stem cells (BMSCs) on articular cartilage defect in rabbits. Rabbit BMSCs were isolated and cultured, and they were then inoculated on HAAM to prepare the complex of HAAM and BMSCs. Twenty-four rabbits were randomly divided into groups A and B, with 12 animals in each group. The left and right sides were used as the experimental and control sides, respectively. The models of bilateral articular cartilage defect were established.

Gene expression during induced differentiation of sheep bone marrow mesenchymal stem cells into osteoblasts

Y. Hu, Tang, X. - X., and He, H. - Y., Gene expression during induced differentiation of sheep bone marrow mesenchymal stem cells into osteoblasts, vol. 12, pp. 6527-6534, 2013.

The aim of this study was to observe the dynamic expression of osteopontin, type I collagen, and osteocalcin genes during the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts and confirm whether BMSCs can differentiate and mature into osteoblasts. A healthy, 2-month-old Altay tailed sheep was obtained, and 10 mL bone marrow was extracted from the posterior superior iliac spine of the sheep via puncture. Sheep BMSCs were extracted using a whole bone marrow culture method and cultured for osteogenic induction.

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