Bovine

Evaluation of the semen swim-up method for bovine sperm RNA extraction

C. M. Han, Chen, R., Li, T., Chen, X. L., Zheng, Y. F., Ma, M. T., Gao, Q. H., Han, C. M., Chen, R., Li, T., Chen, X. L., Zheng, Y. F., Ma, M. T., and Gao, Q. H., “Evaluation of the semen swim-up method for bovine sperm RNA extraction”, vol. 15, p. -, 2016.

Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the E.Z.N.A.TM Total RNA kit II, with non-swim-up sperm as a control.

Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells

Q. D. Jiang, Li, H. P., Liu, F. J., Wang, X. J., Guo, Y. J., Wang, L. F., Lu, W. F., Li, H. J., Li, X. P., and Wang, Y. Y., “Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells”, vol. 13, pp. 2548-2554, 2014.

This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 106 cells per dish). The test was divided into 6 groups with 6 replications per group. The 6 groups were treated with the following volumes of LPS (0.1 mg/mL): 0, 1, 1.5, 2, 4, and 8 μL. The cells were cultured for 24 h, and the total RNA was extracted from samples.

Luteinizing hormone receptor splicing variants in bovine Leydig cells

T. H. Ma, Xiong, Q. H., Yuan, B., Jiang, H., Gao, Y., Xu, J. B., Liu, S. Y., Ding, Y., Zhang, G. L., Zhao, Y. M., and Zhang, J. B., “Luteinizing hormone receptor splicing variants in bovine Leydig cells”, vol. 11, pp. 1721-1730, 2012.

The luteinizing hormone receptor (LHR) plays a key role in testosterone production through its interaction with the gonadotropins, LH and chorionic gonadotropin. We examined the LHR splicing pattern in bovine Leydig cells; LH-induced expression of eight cloned splicing variants was detected by real-time PCR. Luteinizing hormone applied to cultured Leydig cells resulted in expression of full-length LHR and the A and B isoforms, as well as secretion of testosterone, which first increased, then declined, and then increased further, with increased LH levels.

Novel SNPs of the mannan-binding lectin 2 gene and their association with production traits in Chinese Holsteins

Z. L. Zhao, Wang, C. F., Li, Q. L., Ju, Z. H., Huang, J. M., Li, J. B., Zhong, J. F., and Zhang, J. B., “Novel SNPs of the mannan-binding lectin 2 gene and their association with production traits in Chinese Holsteins”, vol. 11, pp. 3744-3754, 2012.

The mannan-binding lectin gene (MBL) participates as an opsonin in the innate immune system of mammals, and single nucleotide polymorphisms (SNPs) in MBL cause various immune dysfunctions. In this study, we detected SNPs in MBL2 at exon 1 using polymerase chain reaction single-strand conformation polymorphism analysis and DNA sequencing techniques in 825 Chinese Holstein cows. Four new SNPs with various allele frequencies were also found. The g.1164 G>A SNP was predicted to substitute arginine with glutamine at the N-terminus of the cysteine-rich domain.

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

S. Wei, Zan, L. S., Wang, H. B., Cheng, G., Du, M., Jiang, Z., Hausman, G. J., McFarland, D. C., and Dodson, M. V., “Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes”, vol. 12, pp. 494-505, 2013.

Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine.

Isolation and identification of bovine primary hepatocytes

Q. D. Jiang, Li, H. P., Liu, F. J., Wang, X. J., Guo, Y. J., Wang, L. F., Lu, W. F., Li, H. J., Li, X. P., and Yang, G. Y., “Isolation and identification of bovine primary hepatocytes”, vol. 12, pp. 5186-5194, 2013.

The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and purity are much lower in this condition. On the basis of previous research, in this study, the two-step collagenase perfusion technique was used for isolating hepatocytes.

Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D3 supplementation

L. R. Rezende, Delgado, E. F., Júnior, A. R. L., Gasparin, G., Jorge, E. C., Mourão, G. B., and Coutinho, L. L., “Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D3 supplementation”, vol. 12, pp. 6611-6618, 2013.

In order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter).

Comparative phenotypic profile of subpopulations of peripheral blood leukocytes in European (Bos taurus taurus) and Zebu cattle (Bos taurus indicus)

A. A. Macêdo, Marciano, A. P. V., Rocha, L. M., Alves-Júnior, J. R. F., Faria, A. M. C., Bittar, J. F. F., Araújo, M. S. S., Santos, R. L., and Martins-Filho, O. A., “Comparative phenotypic profile of subpopulations of peripheral blood leukocytes in European (Bos taurus taurus) and Zebu cattle (Bos taurus indicus)”, vol. 12, pp. 6838-6849, 2013.

Differences in cellular and humoral immunity in Zebu (Bos taurus indicus) and European (B. taurus taurus) cattle breeds, which may be related to differences in resistance or susceptibility to infectious or parasitic diseases, are largely unknown.

A single nucleotide polymorphism in the coding region of bovine transferrin is associated with milk fat yield

A. Sanz, Ordovás, L., Serrano, C., Zaragoza, P., Altarriba, J., and Rodellar, C., “A single nucleotide polymorphism in the coding region of bovine transferrin is associated with milk fat yield”, vol. 9, pp. 843-848, 2010.

The bovine transferrin gene (TF) is located at 125 cM on bovine chromosome 1 (BTA1); it codes for transferrin, a glycoprotein that is highly conserved in many species and that is responsible for iron transport. The TF gene has been located in several QTL regions, and some transferrin classes have been associated with fat and milk yields. We analyzed by means of allele-specific oligonucleotide real-time PCR the c.1455A>G SNP in exon 12 of the TF cDNA sequence (accession number U02564), which induces an Asp/Gly substitution at position 469 of the peptide.

Prospecting candidate SNPs for backfat in Canchim beef cattle

G. B. Veneroni, Meirelles, S. L., Grossi, D. A., Gasparin, G., Ibelli, A. M. G., Tizioto, P. C., Oliveira, H. N., Alencar, M. M., and Regitano, L. C. A., “Prospecting candidate SNPs for backfat in Canchim beef cattle”, vol. 9, pp. 1997-2003, 2010.

Canchim is a composite cattle breed developed in Brazil for beef production. One of the breeding objectives is to increase fat deposition. QTLs for fat thickness and/or marbling have been reported on BTA4 and BTA14. The IGFBP3 and DDEF1 genes, mapped to BTA4 and BTA14, respectively, affect adipogenesis. We looked for SNPs in the IGFBP3 and DDEF1 genes that could be associated with backfat thickness in Canchim beef cattle.

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