Bovine milk

Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk

T. Usman, Yu, Y., Liu, C., Fan, Z., and Wang, Y., Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk, vol. 13, pp. 3319-3328, 2014.

Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three methods, Modified Nucleospin Blood Kit method, Modified TianGen Kit method and Phenol-Chloroform method for genomic DNA extraction from bovine milk. Individual cows’ milk and bulk milk samples were collected from a China agricultural university dairy farm.

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