Bovine papillomavirus

Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects

R. P. Araldi, Melo, T. C., Neves, A. C., Spadacci-Morena, D. D., Magnelli, R. F., Modolo, D. G., de-Sá-Júnior, P. L., Mazucchelli-de-Souza, J., Carvalho, R. F., Beçak, W., and Stocco, R. C., Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects, vol. 14, pp. 12942-12954, 2015.

The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. The comet assay (CA) was used to evaluate the clastogenic potential of BPV. The results confirmed the presence of BPV-2, 3, 5, and 9 in infected samples.

Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene

E. G. Lima, Lira, R. C., Jesus, A. L. S., Dhalia, R., and Freitas, A. C., Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene, vol. 13. pp. 1121-1126, 2014.

Papillomaviruses are known to cause tumor lesions, generally benign, in epithelial tissues of diverse organisms; these lesions may progress to cancer under suitable conditions. Bovine papillomavirus (BPV) can cause urinary bladder cancer and cancer of the upper gastrointestinal tract. Furthermore, BPV1 and BPV2 are implicated in the development of tumors in equids. Many studies with animal models clearly demonstrate that DNA vaccines are very effective tools in controlling viral infections, providing strong humoral and cellular immune responses.

Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle

T. C. Melo, Carvalho, R. F., Mazzucchelli-de-Souza, J., Diniz, N., Vasconcelos, S., Assaf, S. L. M. R., Araldi, R. P., Ruiz, R. M., Kerkis, I., Beçak, W., and Stocco, R. C., Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle, vol. 13, pp. 2458-2469, 2014.

Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for identifying BPVs based on PCR-RFLP and DNA sequencing allowed the identification of a new putative Deltapapillomavirus, designated JN/3SP (JQ280500.1).

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

R. P. Araldi, Carvalho, R. F., Melo, T. C., Diniz, N. S. P., Sant’Ana, T. A., Mazzuchelli-de-Souza, J., Spadacci-Morena, D. D., Beçak, W., and Stocco, R. C., Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil, vol. 13, pp. 5644-5653, 2014.

Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn.

Expression of the bovine papillomavirus type 1, 2 and 4 L1 genes in the yeast Pichia pastoris

A. L. S. Jesus, Mariz, F. C., Souza, H. M., Cordeiro, M. N., Coimbra, E. C., Leitão, M. C. G., Nascimento, L. M., Stocco, R. C., Beçak, W., and Freitas, A. C., Expression of the bovine papillomavirus type 1, 2 and 4 L1 genes in the yeast Pichia pastoris, vol. 11, pp. 2598-2607, 2012.

Papillomaviruses are known to cause benign or malignant lesions in various animals. In cattle, bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. Currently, there are no standard diagnostic tests or prophylactic vaccines. Protection against papillomavirus infection is conferred by neutralizing antibodies directed towards the major structural protein L1.

Entropy-based approach for selecting informative regions in the L1 gene of bovine papillomavirus for phylogenetic inference and primer design

M. V. A. Batista, Freitas, A. C., and Balbino, V. Q., Entropy-based approach for selecting informative regions in the L1 gene of bovine papillomavirus for phylogenetic inference and primer design, vol. 12, pp. 400-407, 2013.

Bovine papillomaviruses (BPVs) cause many benign and malignant lesions in cattle and other animals. Twelve BPV types have been identified so far, and several putative novel BPV types have been detected based on the analysis of L1 gene fragments, generated by FAP59/64 and MY11/09 primers. Phylogenetic trees are important in studies that describe novel BPV types. However, topological mistakes could be a problem in such studies. Therefore, we made use of entropy to find phylogenetic informative regions in the BPV L1 gene sequences from all 12 BPVs.

Simultaneous presence of bovine papillomavirus and bovine leukemia virus in different bovine tissues: in situ hybridization and cytogenetic analysis

A. Yaguiu, Dagli, M. L. Z., Birgel, Jr., E. H., Reis, B. C. A. Alves, Ferraz, O. P., Pituco, E. M., Freitas, A. C., Beçak, W., and Stocco, R. C., Simultaneous presence of bovine papillomavirus and bovine leukemia virus in different bovine tissues: in situ hybridization and cytogenetic analysis, vol. 7, pp. 487-497, 2008.

Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, in addition to epithelium. Cytogenetic abnormalities were observed in blood lymphocytes. The presence of more than one virus in a single tissue is a difficult aspect to evaluate,especially when the DNA sequences are detected in tissues that are not specifically targeted by the virus. BPV and bovine leukemia virus (BLV) are clastogenic, causing chromosome aberrations in peripheral blood lymphocytes.

Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals

C. J. Lindsey, Almeida, M. E., Vicari, C. F., Carvalho, C., Yaguiu, A., Freitas, A. C., Beçak, W., and Stocco, R. C., Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals, vol. 8, pp. 310-318, 2009.

Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that characterize the clinical condition associated with chronic enzootic hematuria.

Simultaneous presence of bovine papillomavirus in blood and in short-term lymphocyte cultures from dairy cattle in Pernambuco, Brazil

N. Diniz, Melo, T. C., Santos, J. F., Mori, E., Brandão, P. E., Richtzenhain, L. J., Freitas, A. C., Beçak, W., Carvalho, R. F., and Stocco, R. C., Simultaneous presence of bovine papillomavirus in blood and in short-term lymphocyte cultures from dairy cattle in Pernambuco, Brazil, vol. 8, pp. 1474-1480, 2009.

Bovine papillomaviruses (BPV) are the causal agents of benign and malignant lesions; they can cause dramatic economic losses in cattle. Although 10 virus types have been described, three types are most common in tumors, namely BPV-1, -2 and -4. Previous studies have reported BPV in blood cells and the possibility of blood acting as a latent virus site and/or transmission agent of virus dissemination. We studied a Holstein dairy herd in Pernambuco, Brazil, in which several animals showed severe cutaneous papillomatosis, without previous determination of BPV types.

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