Bradysia hygida

Chromosomal localization and partial sequencing of the 18S and 28S ribosomal genes from Bradysia hygida (Diptera: Sciaridae)

V. P. Gaspar, Shimauti, E. L. T., and Fernandez, M. A., “Chromosomal localization and partial sequencing of the 18S and 28S ribosomal genes from Bradysia hygida (Diptera: Sciaridae)”, vol. 13, pp. 2177-2185, 2014.

In insects, ribosomal genes are usually detected in sex chromosomes, but have also or only been detected in autosomal chromosomes in some cases. Previous results from our research group indicated that in Bradysia hygida, nucleolus organizer regions were associated with heterochromatic regions of the autosomal C chromosome, using the silver impregnation technique. The present study confirmed this location of the ribosomal genes using fluorescence in situ hybridization analysis.

Molecular combing in the analysis of developmentally regulated amplified segments of Bradysia hygida

K. J. R. Passos, Togoro, S. Y., Carignon, S., Koundrioukoff, S., Lachages, A. - M., Debatisse, M., and Fernandez, M. A., “Molecular combing in the analysis of developmentally regulated amplified segments of Bradysia hygida”, vol. 11, pp. 2060-2070, 2012.

Molecular combing technology is an important new tool for the functional and physical mapping of genome segments. It is designed to identify amplifications, microdeletions, and rearrangements in a DNA sequence and to study the process of DNA replication. This technique has recently been used to identify and analyze the dynamics of replication in amplified domains. In Bradysia hygida, multiple amplification initiation sites are predicted to exist upstream of the BhC4-1 gene. However, it has been impossible to identify them using the available standard techniques.

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