Broilers

Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

F. S. Carvalho, Wenceslau, A. A., Teixeira, M., and Albuquerque, G. R., Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus, vol. 10, pp. 996-1005, 2011.

We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR.

Genetic trends of abdominal fat content in a male broiler chicken line

Lde Genova Gaya, Mourão, G. Barreto, de Rezende, F. Marcondes, de Mattos, E. Chicaroni, Filho, T. Michelan, Figueiredo, L. Gustavo Gi, Ferraz, J. Bento Ster, and Eler, J. Pereira, Genetic trends of abdominal fat content in a male broiler chicken line, vol. 4, pp. 760-764, 2005.

Data of chickens from a broiler-breeding program were collected and used to determine the genetic trends of absolute and relative abdominal fat content. The genetic trends were estimated by the regression of trait genetic value averages on hatch-years. Genetic values from 32,485 individuals were used for regression analysis. The genetic trend estimate for absolute abdominal fat content was +0.39 g per year, indicating that abdominal fat deposition in the analyzed line, in absolute terms, tended to increase, making the existing excess fat deposition in the broilers even worse.

Subscribe to Broilers