cDNA-amplified fragment length polymorphism

Differential gene expression in drought-tolerant sugarcane roots

J. S. Vantini, Dedemo, G. C., Gimenez, D. F. Jovino, Fonseca, L. F. S., Tezza, R. I. D., Mutton, M. A., Ferro, J. A., and Ferro, M. I. T., Differential gene expression in drought-tolerant sugarcane roots, vol. 14, pp. 7196-7207, 2015.

Drought is one of the most frequent abiotic stresses limiting the productivity and geographical distribution of sugarcane culture. The use of drought-tolerant genotypes is one approach for overcoming the effects of water stress. We conducted a comparative study to identify gene expression profiles under water stress in tolerant sugarcane roots. Two different cultivars, 1 drought tolerant (RB867515) and 1 drought susceptible (SP86-155), were evaluated at 4 sampling time points (1, 3, 5, and 10 days) using the cDNA-amplified fragment length polymorphism technique.

Isolation and expression analysis of the soybean GmPic gene

A. - H. Sha, Wu, H., Fu, X. - M., Zhang, Q. - L., Guo, Q. - L., Chen, Y. - H., and Zhou, X. - A., Isolation and expression analysis of the soybean GmPic gene, vol. 13, pp. 4380-4391, 2014.

The differential screening method was used to isolate the soy photoperiodic response-related genes and to further elucidate the molecular mechanisms of the soybean photoperiodic response. The light-sensitive species Zhong Dou 24 was used to receive long-time sunshine, short-time sunshine, and natural sunshine treatment. The cDNA-amplified fragment length polymorphism technique was used to screen the differentially expressed cDNA fragments. The rapid amplification of cDNA end technique was used to isolate the gene.

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