CDV

Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection

H. L. Del Puerto, Martins, A. S., Braz, G. F., Alves, F., Heinemann, M. B., Rajão, D. S., Araújo, F. C., Martins, S. F., Nascimento, D. R., Leite, R. C., and Vasconcelos, A. C., Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection, vol. 10, pp. 2527-2533, 2011.

We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a control. Total RNA was isolated from Vero cells using 1 mL Trizol® LS, and RT was performed using 2 μg total RNA.

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