Cell proliferation

Effect of p53 gene polymorphism on functions of prostate cancer cells

N. Chi, Yun, Z. Z., Tan, Z. H., Li, X. Z., Chen, B. T. B. G., Liu, J., Xu, L. B., Ma, K. W., Li, S. X., Liu, J. F., and Liu, C. X., Effect of p53 gene polymorphism on functions of prostate cancer cells, vol. 14, pp. 11700-11709, 2015.

Prostate cancer cells were transfected with plasmids [empty plasmids, wild-type pcDNA3.1-p53 (V/V), mutant type pcDNA3.1- p53 (G/G)] to analyze the effect of p53 gene polymorphisms on the proliferation, cycle, and apoptosis of prostatic cancer cells. Empty plasmids containing wild-type pcDNA3.1-p53 (V/V) and mutant type pcDNA3.1- p53 (G/G) were used to transfect PC3 and LNCaP cells, respectively. Cell proliferation was detected at 0, 24, 48, and 72 h using the MTT method. Cells were collected at 24 and 72 h.

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