Cell suspension

DNA extraction from fixed cytogenetic cell suspensions

M. R. Amorim, Vargas, F. R., Junior, J. C. Llerena, and Pombo-de-Oliveira, M. S., DNA extraction from fixed cytogenetic cell suspensions, vol. 6, pp. 500-503, 2007.

We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing.

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