Chang liver cells

Establishment of a hepatocyte steatosis model using Chang liver cells

D. Yan, Dou, Q. L., Wang, Z., and Wei, Y. Y., Establishment of a hepatocyte steatosis model using Chang liver cells, vol. 14, pp. 15224-15232, 2015.

The objective of this study was to explore the experimental conditions for hepatocellular steatosis models of Chang liver cells induced by oleic acid (OA). For that, Chang liver cells were induced by different concentrations of OA for different periods. The MTT assay was used to detect hepatic cell activity, the Oil Red O staining was used to observe intracellular lipid droplets accumulation, and the glycerol phosphate oxidase method was used to detect the triglyceride (TG) content in the Chang liver cell.

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